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Plasmid#112995PurposeP element vector for transposon insertion of full-length Drosophila SuUR into Drosophila genomeDepositorInsertSuUR (SuUR Fly)
UseP insertion vectorAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-ABE N-terminal
Plasmid#137177PurposeAAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoterDepositorInsertv5 AAV-ABE N-terminal
UseAAVMutationCas9 D10APromoterCbhAvailable SinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-SaKKHABE8e-bGH-U6-sgRNA-BsmBI
Plasmid#189923PurposeAAV genome encoding SaKKHABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installationDepositorInsertSaABE8e V106W
UseAAVMutationnSaCas9 KKH, TadA ABE8e V106WPromoterEFSAvailable SinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
eeBxb1_IVT
Plasmid#222342PurposePlasmid for evolved and engineered Bxb1 mRNA in vitro transcription templateDepositorInsertCodon-optimized evolved and engineered Bxb1
UseTemplate for in vitro transcriptionPromoterT7 (inactivated)Available SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-SauriABE8e-bGH-U6-sgRNA-BsmBI
Plasmid#189925PurposeAAV genome encoding SauriABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installationDepositorInsertSauriABE8e
UseAAVMutationSauriCas9 D15APromoterEFSAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-Hsp-eGFP-PGK-mCherry
Plasmid#191862PurposeHeat inducible eGFP reporter with constitutive mCherry markerDepositorInsertseGFP
mCherry
UseLentiviralExpressionMammalianPromoterHSPA7 promoter and PGK promoterAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-dual-CRISPR-U6-H1(pBP43)
Plasmid#218930PurposeThe backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterhuman U6 and H1Available SinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAWPSG-Po-nCas9-BE
Plasmid#195740PurposeExpress APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coliDepositorArticleInsertDi-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPo (phoenol-inducible promoter)Available SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-dual-scafffold-2.0 (pBP49)
Plasmid#218931PurposeThe dual-scaffold for making dual-CRISPR screening libraries or dual-CRISPR plasmids.DepositorInsertdual scaffolds for CRISPR
UseCRISPRAvailable SinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-SaABE8e-bGH-U6-sgRNA-BsmBI
Plasmid#189922PurposeAAV genome encoding SaABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installationDepositorInsertSaABE8e
UseAAVMutationSaCas9 D10APromoterEFSAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGF1-4eCOL2A1
Plasmid#97210PurposeLentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoterDepositorInsert4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
UseLentiviral and LuciferaseExpressionMammalianPromoterCOL2A1 regulatory elementsAvailable SinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPPC010.AAV
Plasmid#171175PurposeExpression of Sp.pCas9-dCas9, BBa_J23107-MCP-SoxS(R93A/S101A), and hAAVS1 scRNA on pBBR1-KmR plasmidDepositorInsertshAAVS1 scRNA
Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPRExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterBBa_J23119 and Sp.pCas9, BBa_J23107Available SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC57-4eCOL2A1
Plasmid#97211PurposeFor cloning of a COL2A1-based, chondrogenesis-responsive promoter, and subcloning this insert into new vectorsDepositorInsert4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
UseVector is designed for cloning and generation of …PromoterCOL2A1 regulatory elementsAvailable SinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-SaABE8eV106W-bGH-U6-sgRNA-BsmBI
Plasmid#189924PurposeAAV genome encoding SaABE8eV106W and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installationDepositorInsertSaABE8e V106W
UseAAVMutationSaCas9 D10A, TadA ABE8e V106WPromoterEFSAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-egfp-U3-Osp.gRNA
Plasmid#166034PurposeEmpty backbone for expression of gRNA-expressing integration deficient lentiviral particles.DepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-saBE3.9max C-terminal
Plasmid#137180PurposeExpresses the C-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoterDepositorInsertNpu-saBE3.9max_C-terminal
ExpressionMammalianPromoterCMVAvailable SinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-saBE3.9max N-terminal
Plasmid#137179PurposeExpresses the N-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoterDepositorInsertNpu-saBE3.9max_N-terminal
ExpressionMammalianMutationCas9 D10APromoterCMVAvailable SinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-nsp7-nsp8
Plasmid#159092PurposeCoexpression construct of Nsp7 with an N-terminal His-tag and Nsp8DepositorInsertsNSP7
NSP8
TagsHisExpressionBacterialPromoterT7Available SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
medSIIN
Plasmid#185664PurposeshApc-expressing lentiviruses with varius levels of expression of SIINFEKLDepositorInsertsmScarlet
miR-30a
shApc
UseLentiviralPromoterEf1aAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
U6-pegRNA-H1-nick sgRNA-mCherry
Plasmid#176901PurposeExpresses prime editing guide RNA and guide RNA in mammalian cellsDepositorInsertsmCherry
gRNA scaffold
gRNA scaffold
UseAAVExpressionMammalianPromoterCAG, H1, and U6Available SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-Hsp-Fluc-PGK-Rluc-mCherry
Plasmid#191863PurposeHeat inducible Fluc reporter with constitutive RlucDepositorInsertsFirefly luciferase Fluc
Renilla luciferase Rluc
UseLentiviralTagsP2A-mCherryExpressionMammalianPromoterHSPA7 promoter and PGK promoterAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)
Plasmid#195741PurposeBase editing for making a pre-stop codon in mmoX using phenol inducible systemDepositorArticleInsertDi-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPo (phoenol-inducible promoter)Available SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
hiSIIN
Plasmid#185662PurposeshApc-expressing lentiviruses with varius levels of expression of SIINFEKLDepositorInsertsmScarlet
miR-30a
shApc
UseLentiviralPromoterEf1aAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
midSIIN
Plasmid#185665PurposeshApc-expressing lentiviruses with varius levels of expression of SIINFEKLDepositorInsertsmScarlet
miR-30a
shApc
UseLentiviralPromoterEf1aAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
dimSIIN
Plasmid#185661PurposeshApc-expressing lentiviruses with varius levels of expression of SIINFEKLDepositorInsertsmScarlet
miR-30a
shApc
GFP
UseLentiviralPromoterEf1aAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAWPSG-Ptet-nCas9-BE
Plasmid#195739PurposeExpress APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coliDepositorArticleInsertTet repressor protein / APOBEC1-nCas9(D10A)-UGI
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPtet(Tetracycline inducible protein)Available SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
loSIIN
Plasmid#185663PurposeshApc-expressing lentiviruses with varius levels of expression of SIINFEKLDepositorInsertsmScarlet
miR-30a
shApc
GFP
UseLentiviralPromoterEf1aAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked Free ClLIS1 (M)
Plasmid#182487PurposeYeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-ClLIS1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertFPPS(M)-LS
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
noSIIN
Plasmid#185666PurposeshApc-expressing lentiviruses with no expression of SIINFEKLDepositorInsertsmScarlet
miR-30a
shApc
UseLentiviralPromoterEf1aAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
p168-759
Plasmid#62619PurposeePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4)DepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
Scaffold Protein encoding GBD:SH3:PDZ (1:1:4)
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialPromoterT7Available SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-BE3.9max N-terminal
Plasmid#137171PurposeExpresses the N-terminal split-intein fragment of BE3.9max from the CMV promoterDepositorInsertNpu-BE3.9max_N-terminal
ExpressionMammalianMutationCas9 D10APromoterCMVAvailable SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-ABEmax N-terminal
Plasmid#137173PurposeExpresses the N-terminal split-intein fragment of ABEmax from the CMV promoterDepositorInsertNpu-ABEmax_N-terminal
ExpressionMammalianMutationCas9 D10APromoterCMVAvailable SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
TS-PE2-C-WPRE
Plasmid#176650PurposeExpresses C-terminus of PE2 in mammalian cellsDepositorInsertsC-terminus of PE2
Artificial SA
WPRE
bGH poly(A)
UseAAVTagsSV40 NLSExpressionMammalianPromoterNoneAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
TS-PE2-N
Plasmid#176649PurposeExpresses N-terminus of PE2 in mammalian cellsDepositorInsertsN-terminus of PE2
Artificial SD
UseAAVTagsSV40 NLSExpressionMammalianPromoterCMV and NoneAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES
Plasmid#182485PurposeYeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS-NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked Free PcPTS
Plasmid#182486PurposeYeast integrative plasmid for expressing fusion protein ERG20-PcPTS, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertFPPS-PTS
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free GFP-NES
Plasmid#182491PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS
GFP-NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10 and GAL7Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-crRNA(Non-targeting)
Plasmid#109324PurposeAAV vector expressing non-targeting AsCpf1 crRNADepositorInsertmCherry-KASH
UseAAVPromoterhSyn1Available SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-saBE3.9max_KKH C-terminal
Plasmid#137181PurposeExpresses the C-terminal split-intein fragment of S. aureus BE3.9max (KKH variant) from the CMV promoterDepositorInsertNpu-saBE3.9max_KKH_C-terminal
ExpressionMammalianMutationE782K;N968K;R1015H conferring recognition of NNNR…PromoterCMVAvailable SinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-GAP-LacI-Mit1AD
Plasmid#126722PurposeLacI-Mit1AD on pGAPZ_ A expression vectorDepositorInserthybrid lacI-Mit1 activation domain coding sequence
ExpressionYeastPromoterGAPAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWT-IFl_WT-IFr
Plasmid#192207PurposeWildtype I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.DepositorInsertWildtype I-F Leader and wildtype I-F Locus DNA
ExpressionBacterialAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pScr-IFl_WT-IFr
Plasmid#192208PurposeScrambled I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.DepositorInsertScrambled I-F Leader and wildtype I-F Locus DNA
ExpressionBacterialAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCon-IFl_Con-IFr
Plasmid#192209PurposeConsensus I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.DepositorInsertConsensus I-F Leader and Consensus I-F Locus DNA
ExpressionBacterialAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCon-IFl_Con-IEr
Plasmid#192210PurposeConsensus I-F CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.DepositorInsertConsensus I-F Leader and Consensus I-E Locus DNA
ExpressionBacterialAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCon-IFl_Con-ICr
Plasmid#192211PurposeConsensus I-F CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.DepositorInsertConsensus I-F Leader and Consensus I-C Locus DNA
ExpressionBacterialAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCon-IFl_Con-IIAr
Plasmid#192212PurposeConsensus I-F CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.DepositorInsertConsensus I-F Leader and Consensus II-A Locus DNA
ExpressionBacterialAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCon-IEl_Con-IEr
Plasmid#192213PurposeConsensus I-E CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.DepositorInsertConsensus I-E Leader and Consensus I-E Locus DNA
ExpressionBacterialAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCon-ICl_Con-ICr
Plasmid#192214PurposeConsensus I-C CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.DepositorInsertConsensus I-C Leader and Consensus I-C Locus DNA
ExpressionBacterialAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCon-IIAl_Con-IIAr
Plasmid#192215PurposeConsensus II-A CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.DepositorInsertConsensus II-A Leader and Consensus II-A Locus DNA
ExpressionBacterialAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX1-IFl_Con-IFr
Plasmid#192216PurposeMutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.DepositorInsertI-F leader and I-F repeat construct not studied further in this paper (X1)
ExpressionBacterialAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only