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We narrowed to 2,431 results for: pET28

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Showing: 1051 - 1100 of 2431 results

  2. pET28-RPL3-Δ1~34,Δ215~284-TwStrep

    Plasmid
    #208630
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa35~398 with deletion of 215~284, fused with Twin Strep tag.
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) Δ1~34, Δ215~284 (RPL3 Human)
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    Δ1~34,Δ215~284
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208630-plasmid-map-sequence-id-418338

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  3. pET28-RPL3-53~77-mEGFP(A206K)-TwStrep

    Plasmid
    #208632
    Purpose
    for E. coli expression truncated human RPL3 (ENST00000216146), aa53~77, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 53~77 (RPL3 Human)
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 53~77, other residues not included
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208632-plasmid-map-sequence-id-418343

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  4. pET28-RPL3-1~77-mEGFP(A206K)-TwStrep

    Plasmid
    #208624
    Purpose
    for E. coli expression truncated human RPL3 (ENST00000216146), aa1~77, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 1~77 (RPL3 Human)
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 1~77, other residues not included
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208624-plasmid-map-sequence-id-418336

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  15. pET28b(+)_S. cerevisiae Hbs1(162-611)

    Plasmid
    #39337
    Depositor
    SeWon Suh
    Insert
    Elongation factor 1 alpha-like protein
    Tags
    His-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 27, 2012
    Availability
    Academic Institutions and Nonprofits only

  21. pJC2527 - pET28-SKP1(A2P, ∆38-43, ∆71-82)-StrepII-FBXO31(∆1-65)

    Plasmid
    #250252
    Purpose
    Recombinant production of SKP1(A2P, ∆38-43, ∆71-82) and 6His-Smt3-StrepII-FBXO31(∆1-65).
    Depositor
    Jacob Corn
    Article
    Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:
    Inserts
    SKP1 (SKP1 Human)
    FBXO31 (FBXO31 Human)
    Tags
    6xHis-Smt3(SUMO)-StrepII
    Expression
    Bacterial
    Mutation
    A2P, ∆38-43, ∆71-82 and ∆1-65
    Promoter
    T7
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    250252-plasmid-map-sequence-id-498556

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  25. pET28-SpRY-HF1-NLS-6xHis (gbr2102)

    Plasmid
    #181744
    Purpose
    bacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    bacterial codon optimized SpRY-HF1
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS(SV40)-6xHis and MKIEE MBP tag
    Expression
    Bacterial
    Mutation
    SpRY-HF1, SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E…
    Promoter
    T7
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    181744-plasmid-map-sequence-id-373171

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  28. pET28a(+)v2_TwinStrep-SUMO-φC31(PhiC31)-8xHis

    Plasmid
    #246982
    Purpose
    Expresses E. coli codon-optimized TwinStrep-SUMO-tagged φC31(PhiC31) integrase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.
    Depositor
    Amro Hamdoun
    Insert
    φC31(PhiC31)
    Use
    Synthetic Biology
    Tags
    8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    246982-plasmid-map-sequence-id-489535

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  34. pET28-His6-SUMO-cSrc-SH2-AviTag

    Plasmid
    #214230
    Purpose
    Bacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Li et al Elife. 2023 Mar 16;12:e82345. doi: 10.75
    Insert
    cSrc kinase SH2 domain (SRC Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    214230-plasmid-map-sequence-id-429073

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  38. pET28a-Coh-ddFLN4-ELP-HIS-ybbr

    Plasmid
    #153446
    Purpose
    E. coli expression of Rc. CohE wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.CohE
    Tags
    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    July 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    153446-plasmid-map-sequence-id-306487

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  41. pJC2747 - pET28-SKP1(A2P, ∆38-43, ∆71-82)-FBXO31(∆1-65, D334N)

    Plasmid
    #250278
    Purpose
    Recombinant production of SKP1(A2P, ∆38-43, ∆71-82) and 6His-Smt3-StrepII-FBXO31(∆1-65, D334N).
    Depositor
    Jacob Corn
    Article
    Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:
    Inserts
    SKP1 (SKP1 Human)
    FBXO31 (FBXO31 Human)
    Tags
    6xHis-Smt3(SUMO)-StrepII
    Expression
    Bacterial
    Mutation
    A2P, ∆38-43, ∆71-82 and ∆1-65, D334N
    Promoter
    T7
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    250278-plasmid-map-sequence-id-498555

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  43. pET28-His6-SUMO-Lyn-SH2-AviTag

    Plasmid
    #214207
    Purpose
    Bacterial expression of SH2 domain of the Lyn kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    Lyn A (LYN Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214207-plasmid-map-sequence-id-422791

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  44. pET28-His6-SUMO-Yes-SH2-AviTag

    Plasmid
    #214208
    Purpose
    Bacterial expression of SH2 domain of the Yes kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    Yes kinase SH2 domain (YES1 Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214208-plasmid-map-sequence-id-422836

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  45. pET28-His6-SUMO-Fyn-SH2-AviTag

    Plasmid
    #214202
    Purpose
    Bacterial expression of SH2 domain of the Fyn kinase (B- iso) with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    FYN kinase (FYN Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214202-plasmid-map-sequence-id-422824

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  46. pET28-His6-SUMO-Lck-SH2-AviTag

    Plasmid
    #214203
    Purpose
    Bacterial expression of SH2 domain of the Lck kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    Lck kinase SH2 domain (LCK Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214203-plasmid-map-sequence-id-422773

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  47. pET28-His6-SUMO-Blk-SH2-AviTag

    Plasmid
    #214204
    Purpose
    Bacterial expression of SH2 domain of the Blk kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    Blk kinase SH2 domain (BLK Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214204-plasmid-map-sequence-id-422774

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  48. pET28-His6-SUMO-Fgr-SH2-AviTag

    Plasmid
    #214205
    Purpose
    Bacterial expression of SH2 domain of the Fgr kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    Fgr kinase SH2 domain (FGR Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214205-plasmid-map-sequence-id-422780

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  49. pET28-His6-SUMO-Hck-SH2-AviTag

    Plasmid
    #214206
    Purpose
    Bacterial expression of SH2 domain of the Hck kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    p60-HCK (HCK Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214206-plasmid-map-sequence-id-422790

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Showing: 1051 - 1100 of 2431 results