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pU6-PspCas13b-GEMSgRNA2

Plasmid

#204768

Purpose

pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

Depositor

Kate Meyer

Article

Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

guide RNA targeting GEMS m6A sensor mRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pY2F

Plasmid

#53285

Purpose

Contains an Arabidopsis thaliana lcyE cDNA and produces an active lycopene epsilon-cyclase enzyme in E. coli. Use with pAC-BETA-At or pAC-BETAipi-At to produce alpha-carotene in E. coli.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

lcyE (LUT2 Mustard Weed)

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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Fz4-GFP

Plasmid

#42197

Depositor

Jeremy Nathans, Robert Lefkowitz

Article

Chen et al Science. 2003 Sep 5;301(5638):1391-4.

Insert

Frizzled 4 (Fzd4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pTN4

Plasmid

#174212

Purpose

Donor plasmid for introducing Peft-3::AtTIR1(F79G)::mRuby at the ttTi5605 locus

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Inserts

AtTIR1(F79G)::mRuby (TIR1 Nematode)
GFP

Use

CRISPR and Cre/Lox

Tags

mRuby

Expression

Worm

Mutation

Promoter

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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AAV2-miniSOG-VAMP2-T2A-mCherry

Plasmid

#50970

Purpose

AAV2 transfer vector containing the InSynC (miniSOG-VAMP2-T2A-mCherry) construct

Depositor

Roger Tsien

Article

Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

Insert

miniSOG-VAMP2-T2A-mCherry (Vamp2 Mouse, Arabdopsis thaliana)

Use

AAV

Tags

T2A-mCherry and miniSOG

Expression

Mutation

Promoter

human synapsin promoter

Available since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2-hDKK2-flag

Plasmid

#15495

Depositor

Sergei Sokol

Article

Krupnik et al Gene. 1999 Oct 1. 238(2):301-13.

Insert

DKK2 (DKK2 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pSLQ5079_pHR_PGK_sfGFP_CoV-F1

Plasmid

#155303

Purpose

SARS-CoV-2 fluorescent reporter 1

Depositor

Stanley Qi

Article

Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

Insert

Superfolder GFP fused with SARS-CoV-F1 (ORF1ab Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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SIV_Rev

Plasmid

#236244

Purpose

3rd generation SIV-based lentiviral packaging plasmid; Contains SIV Rev

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

SIV Rev

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-ORF9b

Plasmid

#165122

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

ORF9b

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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FLS2-YFPc

Plasmid

#87166

Purpose

fluorescence complementation assay

Depositor

Silke Robatzek

Article

Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

Insert

FLS2 (FLS2 Mustard Weed)

Use

Tags

YFPc

Expression

Mutation

Promoter

35s

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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FLS2-YFPn

Plasmid

#87165

Purpose

fluorescence complementation assay

Depositor

Silke Robatzek

Article

Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

Insert

FLS2 (FLS2 Mustard Weed)

Use

Tags

YFPn

Expression

Mutation

Promoter

35s

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-Orf9b L52D

Plasmid

#165129

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Orf9b L52D

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pT2U2-YFP

Plasmid

#84544

Purpose

Hybrid reporter construct with 4 transcription factor binding sites (2 TetO, 2 UAS) driving EYFP

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

T2U2-EYFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pU2T4U2-YFP

Plasmid

#84551

Purpose

Hybrid reporter construct with 8 transcription factor binding sites (2 UAS, 4 TetO, 2 UAS) driving EYFP

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

U2T4U2-EYFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pT2U4T2-YFP

Plasmid

#84553

Purpose

Hybrid reporter construct with 8 transcription factor binding sites (2 TetO, 4 UAS, 2 TetO) driving EYFP

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

T2U4T2-EYFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pU2T2-YFP

Plasmid

#84543

Purpose

Hybrid reporter construct with 4 transcription factor binding sites (2 UAS, 2 TetO) driving EYFP

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

U2T2-EYFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV2 ORF8 C-V5-tag

Plasmid

#179990

Purpose

Mammalian expression vector for SARS-CoV-2 ORF8, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-2 ORF8 (ORF8 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV2 ORF7a C-V5-tag

Plasmid

#179981

Purpose

Mammalian expression vector for SARS-CoV-2 ORF7a, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-2 ORF7a (ORF7a Synthetic)

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV2 N C-V5-tag

Plasmid

#179979

Purpose

Mammalian expression vector for SARS-CoV-2 N, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-2 N (N Synthetic)

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV2 NSP16 C-V5-tag

Plasmid

#179976

Purpose

Mammalian expression vector for SARS-CoV-2 Nsp16, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-2 Nsp16 (ORF1ab Synthetic)

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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