We narrowed to 4,380 results for: ARA-2

1. Flag‐Tim2

   Plasmid

   #49213

   Purpose

   expresses Flag tagged Tim2

   Depositor

   Lawrence Kane

   Article

   Knickelbein et al J Immunol. 2006 Oct 15;177(8):4966-70.

   Insert

   Tim2 (Timd2 Mouse)
   

   Tags

   CD8 signal sequence and FLAG

   Expression

   Mammalian

   Mutation

   no Tim2 signal sequence; R80L and S196D compared …

   Promoter

   EF1a

   Available Since

   Dec. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pCG_SARS-CoV2 NSP7/bat-CoV RaTG13 C-V5-tag

   Plasmid

   #179974

   Purpose

   Mammalian expression vector for SARS-CoV-2 Nsp7 or bat RaTG13 Nsp7, V5-tagged (SARS-CoV-2 Nsp7 and RaTG13 Nsp7 have the same amino acid sequence and codon optimized sequence).

   Depositor

   Konstantin Sparrer

   Article

   Hayn et al Cell Rep. 2021 May 18;35(7):109126.

   Insert

   SARS-CoV-2 Nsp7 (ORF1ab Synthetic)
   

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Available Since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7-SpCas9_sgRNA-site2 (RTW448)

   Plasmid

   #160137

   Purpose

   T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)
   

   Use

   In vitro transcription of sgrna from t7 promoter

   Promoter

   T7

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-Bmpr2ΔKinase gRNA resistant

   Plasmid

   #163411

   Purpose

   Expresses BMPR-2ΔKinase resistant to the gRNAs(#163388-90) in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Expression

   Mammalian

   Mutation

   The kinase domain (202-500 aa) is deleted. gRNA-t…

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-Bmpr2ΔEC gRNA resistant

   Plasmid

   #163413

   Purpose

   Expresses BMPR-2ΔEC resistant to the gRNAs(#163388-90) in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Expression

   Mammalian

   Mutation

   The extracellular domain (27-150 aa) is deleted. …

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-Bmpr2ΔTail gRNA resistant

   Plasmid

   #163412

   Purpose

   Expresses BMPR-2ΔTail resistant to the gRNAs(#163388-90) in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Expression

   Mammalian

   Mutation

   The C-terminal tail domain (530-1038 aa) is delet…

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Bmp8a

   Plasmid

   #163592

   Purpose

   Expresses BMP-8A in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmp8a (Bmp8a Mouse)
   

   Expression

   Mammalian

   Available Since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHis-TEV-Nsp15

   Plasmid

   #187656

   Purpose

   E. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

   Depositor

   Debra Hansen

   Article

   Jernigan et al Structure. 2022 Dec 30:S0969-2126(22)004

   Insert

   Nsp15 (N Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   TEV protease cleavable His6-tag

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7lac

   Available Since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDe-CAS9-D10A

   Plasmid

   #61477

   Purpose

   based on pDe-CAS9, encodes Cas9 D10A nickase variant

   Depositor

   Holger Puchta

   Article

   Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

   Insert

   Cas9-D10A
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   catalytic Asp10 changed to Ala

   Promoter

   PcUbi4-2

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGG-PIP37

    Plasmid

    #196775

    Purpose

    Contains LECRK-VI.2 promoter

    Depositor

    Marc Somssich

    Article

    Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

    Insert

    L-TYPE LECTIN RECEPTOR KINASE-VI.2
    

    Use

    Greengate (gg) entry vector

    Available Since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGFPmAID_htz-1

    Plasmid

    #174214

    Purpose

    Donor plasmid for tagging htz-1 with GFP::mAID

    Depositor

    Masato Kanemaki

    Article

    Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

    Insert

    GFP::mAID::htz-1 tagging donor
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTK73_htz-1

    Plasmid

    #174546

    Purpose

    htz-1 targeting gRNA expression

    Depositor

    Masato Kanemaki

    Article

    Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

    Insert

    htz-1 targeting gRNA
    

    Expression

    Worm

    Promoter

    Ce-U6 promoter

    Available Since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAGT9569

    Plasmid

    #219431

    Purpose

    Transcriptional unit (MoClo-position 2): pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOS

    Depositor

    Tom Schreiber

    Article

    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

    Insert

    LB_AtRPS5a:PapE-4xLF2-NLS-SpCas9i-NLS:tNOS_RB (Position 2)
    

    Use

    CRISPR and Synthetic Biology; Moclo compatible le…

    Tags

    PapE-4LF2

    Expression

    Plant

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AP2u2-CMV-Ace2-IgG1

    Plasmid

    #226455

    Purpose

    Mammalian expression of human Ace2 protein under CMV promoter fused with IgG1, tagged with 6His, and released extracellular environment after expression for protein purification

    Depositor

    Urartu Seker

    Article

    Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(

    Inserts

    Angiotensin Converting Enzyme 2
    Immunoglobulin Heavy Constant Gamma 1
    

    Tags

    6xHis Tag

    Expression

    Mammalian

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. Spike Display_6P-D614G

    Plasmid

    #172734

    Purpose

    Expression vector for SARS-CoV-2 (HexaPro-D614G) spike used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CMV

    Available Since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. Ssh2 gRNA#1

    Plasmid

    #163397

    Purpose

    Cas9-mediated knockout of Ssh2 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Ssh2 (Ssh2 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ssh2 gRNA#3

    Plasmid

    #163399

    Purpose

    Cas9-mediated knockout of Ssh2 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Ssh2 (Ssh2 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-Gdf2

    Plasmid

    #163595

    Purpose

    Expresses GDF-2 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Gdf2 (Gdf2 Mouse)
    

    Expression

    Mammalian

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-Bmp2

    Plasmid

    #163587

    Purpose

    Expresses BMP-2 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmp2 (Bmp2 Mouse)
    

    Expression

    Mammalian

    Available Since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFP.R375

    Plasmid

    #138245

    Purpose

    Expresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(SNL).NrdA-2*.(CNE)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    P(araBAD) and iPC

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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