We narrowed to 4,325 results for: PC

1. pET15b-At 14-3-3 phi

   Plasmid

   #44939

   Depositor

   Robert Ferl

   Article

   Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

   Insert

   14-3-3 phi (GF14 PHI Mustard Weed)
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 14, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pET15b-At 14-3-3 lambda

   Plasmid

   #44942

   Depositor

   Robert Ferl

   Article

   Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

   Insert

   14-3-3 lambda (GRF6 Mustard Weed)
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 14, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pAAV_FLAG_hSF3B1

   Plasmid

   #176590

   Purpose

   Adenoviral construct to insert a N-terminal FLAG to one allele of human SF3B1

   Depositor

   Manoj Pillai

   Article

   Boddu et al Commun Biol. 2021 Oct 13;4(1):1184. doi:

   Insert

   SF3B1 (SF3B1 Human)
   

   Use

   AAV

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-UTX(420-633)-H6

   Plasmid

   #183773

   Purpose

   Express pET-UTX(420-633)-H6 in E.coli. Purified pET-UTX(420-633)-H6 was used for generating antibodies in rabbits.

   Depositor

   Peter Harte

   Article

   Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

   Insert

   UTX
   

   Tags

   6 His tag at the C-terminus

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAct-GST-H3(K27Q)

   Plasmid

   #182843

   Purpose

   a K27Q point mutation (AAG/CAG) of Drosophila histone H3 was generated from pAct-GST-H3. Expression in S2 cells.

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2009 Sep;136(18):3131-41. d

   Insert

   GST-histone H3 fusion
   

   Tags

   GST

   Expression

   Insect

   Promoter

   Actin5C

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAct-GST-H3

   Plasmid

   #182842

   Purpose

   A PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2009 Sep;136(18):3131-41. d

   Insert

   GST-histone H3 fusion
   

   Tags

   GST

   Expression

   Insect

   Promoter

   Actin5C

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a_6HT-TBP6.7(P46A)

   Plasmid

   #112721

   Purpose

   This mutation (P46A) marks the first residue within the evolved region of U1A.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(P46A)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   P46A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a_6HT-TBP6.7(Q48T)

   Plasmid

   #112725

   Purpose

   This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q48T)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q48T

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a_6HT-TBP6.7(P51A)

   Plasmid

   #112728

   Purpose

   Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(P51A)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   P51A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a_6HT-TBP6.7(Q54A)

    Plasmid

    #112730

    Purpose

    Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q54A)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q54A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21-pelB-LaM-4

    Plasmid

    #172770

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-4
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57.1_AOX-FLAG

    Plasmid

    #177984

    Purpose

    All-in-one dox-inducible expression of Alternative Oxidase

    Depositor

    David Sabatini, Jessica Spinelli

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    AOX
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Codon optimized cDNA for expression in human cells

    Promoter

    TRE

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21-pelB-LaG-16-4GS-LaG-2

    Plasmid

    #172764

    Purpose

    Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16-4GS-LaG-2
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21-pelB-LaG-16-3xFLAG-LaG-2

    Plasmid

    #172765

    Purpose

    Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (3xFLAG linker)

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16-3xFLAG-LaG-2
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUltra-sY

    Plasmid

    #82417

    Purpose

    Expresses Mj aaRS for sulfotyrosine and Mj tRNA for decoding UAG codons

    Depositor

    Chang Liu

    Article

    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

    Insert

    Methanococcus jannaschii aaRS for sulfotyrosine
    

    Expression

    Bacterial

    Mutation

    Tyr32Leu, Leu65Pro, Asp158Gly, Ile159Cys, Leu162L…

    Promoter

    tacI

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21-pelB-LaG-16

    Plasmid

    #172746

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21-pelB-LaM-6

    Plasmid

    #172771

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-6
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lyn FAK biosensor

    Plasmid

    #78299

    Purpose

    FRET biosensor. Visualization of FAK activity at lipid rafts in live cells

    Depositor

    Yingxiao Wang

    Article

    Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

    Insert

    Lyn Fak biosensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. kiCAP-AAV-MDV1A

    Plasmid

    #196679

    Purpose

    Rep/Cap plasmid for the production of MDV1A, an AAV capsid with CNS tropism in mice.

    Depositor

    Pardis Sabeti

    Article

    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Mutation

    RSVGSVY insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. KRas FAK biosensor

    Plasmid

    #78301

    Purpose

    FRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells

    Depositor

    Yingxiao Wang

    Article

    Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

    Insert

    KRas FAK biosensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only