We narrowed to 792 results for: akt
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Plasmid#184054PurposeLysosome-targeted constitutively active mutant of phosphatase and tensin homolog (PTEN); tagged with mCherry.DepositorInsertLyso-PTEN(A4)-mCherry (PTEN Human)
TagsLysosome-associated membrane protein 1 (LAMP1). a…ExpressionMammalianMutationSer 380/385 and Thr 382/383 in PTEN mutated to Al…PromoterCMVAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-INPP4B-mCherry
Plasmid#184055PurposeLysosome targeted inositol polyphosphate-4-phosphatase type II B; tagged with mCherry.DepositorTagsLysosome-associated membrane protein 1 (LAMP1). a…ExpressionMammalianPromoterCMVAvailable SinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 (+) FLAG-Ago2 S387E
Plasmid#60653PurposeExpresses CMV-FLAG-Ago2 (N-term tag) S387E mutant in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
TagsCMV-FLAG-Ago2 (N-term tag)ExpressionMammalianMutationS387E mutantPromoterCMVAvailable SinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 (+) FLAG-Ago2 S387A
Plasmid#60652PurposeExpresses CMV-FLAG-Ago2 (N-term tag) S387A mutant in mammalian cellsDepositorAvailable SinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST26 Ago2 S387A
Plasmid#59905PurposeExpresses 6xHis-Ago2 (N-termi tag) S387A mutant in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
Tags6xHis (N-termi tag)ExpressionMammalianMutationS387A, phospho-null alaninePromoterCMVAvailable SinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST26 Ago2 5A
Plasmid#60651PurposeExpresses 6xHis-Ago2 (N-termi tag) mutated five serines to alanines in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
Tags6xHis (N-termi tag)ExpressionMammalianMutationmutated five serines to alaninesPromoterCMVAvailable SinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST26 Ago2 S387E
Plasmid#59906PurposeExpresses 6xHis-Ago2 (N-termi tag) S387E mutant in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
Tags6xHis (N-termi tag)ExpressionMammalianMutationS387E, phospho-dominantPromoterCMVAvailable SinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST26 Ago2 4A
Plasmid#59907PurposeExpresses 6xHis-Ago2 (N-termi tag) mutated four serines to alanines in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
Tags6xHis (N-termi tag)ExpressionMammalianMutationmutated four serines to alaninesPromoterCMVAvailable SinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-TSC2(AA)
Plasmid#192442PurposeEncodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.DepositorInsertmCherry-TSC2(S939A,T1439A) (TSC2 Human)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …PromoterCMVAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-TSC2(AA)-NLS
Plasmid#192443PurposeEncodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.DepositorInsertmCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …PromoterCMVAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
GAG-PKB-pcDNA3
Plasmid#24651DepositorAvailable SinceJune 7, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSi-check2-hRac2 3'UTR
Plasmid#97160PurposeFor dual luciferase report assayDepositorInsertRAC 3'UTR (AKT1 Human)
ExpressionMammalianAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-TSC2(AA,3Q)-NLS
Plasmid#192445PurposeEncodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity; targeted to the nucleus.DepositorInsertmCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q )-NLS (TSC2 Human)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …PromoterCMVAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-TSC2(AA,3Q)
Plasmid#192444PurposeEncodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity.DepositorInsertmCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q ) (TSC2 Human)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …PromoterCMVAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSi-check2-hRac2 - mut 3'UTR
Plasmid#97161PurposeFor dual luciferase report assayDepositorAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPGK-empty
Plasmid#35094DepositorInsertdeletion of zinc fingers and nuclease
TagsHAExpressionMammalianMutationuse as a transfection balance for pPGK-ZFN plasmi…PromoterPGKAvailable SinceMay 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPGK-GZF1-RR
Plasmid#35092DepositorInsertZFN GZF1-Fok(RR)
TagsHA and SV40 NLSExpressionMammalianMutationcontains the "RR" obligate heterodimer …PromoterPGKAvailable SinceMay 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPGK-GZF3-DD
Plasmid#35093DepositorInsertZFN GZF3-Fok(DD)
TagsHA and SV40 NLSExpressionMammalianMutationcontains the "DD" obligate heterodimer …PromoterPGKAvailable SinceMay 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSSA-1-3
Plasmid#35091DepositorInsertSplit luciferase for GZF1/3 SSA assay
ExpressionMammalianMutationSingle strand annealing (SSA) recombinaion report…PromoterSV40Available SinceMarch 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCSC-NLS-EGFP
Plasmid#221811PurposeLentiviral vector expressing green fluorescent protein (GFP) fused with nuclear localization sequence (NLS)DepositorInsertNLS-EGFP
UseLentiviralTagsNLSExpressionMammalianPromoterCMVAvailable SinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only