We narrowed to 2,254 results for: levi

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pU6-Sp-gRNA-IDS_DF_C2c_attB_rev

Plasmid

#182151

Purpose

To insert attB-rev attachment site at IDS in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

IDS-C2c-attBrev pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-IDS2_DF_B7b_attP_fwd

Plasmid

#182150

Purpose

To install attP-fwd attachment site at IDS2 in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

IDS2-B7b-attP-fwd pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-GG-acceptor

Plasmid

#132777

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

Exchangeable cassette

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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v3em-Nterm-PE2max

Plasmid

#198734

Purpose

AAV genome encoding N-terminal PE2max

Depositor

David Liu

Article

Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

Insert

Nterm PE2max-NpuN

Use

AAV

Promoter

Cbh

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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v1em-Cterm-PE2max-U6-pegRNA

Plasmid

#198733

Purpose

AAV genome encoding C-terminal PE2max and U6 expression cassette

Depositor

David Liu

Article

Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

Insert

NpuC-CtermPE2max

Use

AAV

Promoter

EFS

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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Factor IX donor

Plasmid

#182141

Purpose

Factor IX donor for twinPE and Bxb1 mediated insertion at ALB

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attP-Factor IX intron 1 and exon 2-8 CDS and exon 8 3'UTR

Expression

Mammalian

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-pegRNA-HEK3_CTT_ins

Plasmid

#132778

Purpose

S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

HEK3_CTT_ins pegRNA

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-pegRNA-RNF2_+5GtoT

Plasmid

#135957

Purpose

S. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editing

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_5GtoT pegRNA

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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MG15-luc (mut p53 binding sites)

Plasmid

#16443

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

p53-binding site (mutant) (TP53 )

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pU6-sp-sgRNA-RNF2_+41nick

Plasmid

#135958

Purpose

S. pyogenes sgRNA for +41 nick in RNF2

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_+41 nicking sgRNA

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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FRAP

Plasmid

#1827

Depositor

Philip Leder

Article

Ornitz et al Mol Cell Biol 1992 Jan;12(1):240-7.

Insert

FR1 AP (Fgfr1 Mouse)

Expression

Mammalian

Available Since

May 4, 2005

Availability

Academic Institutions and Nonprofits only

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pBS hIhh (CT#402)

Plasmid

#13995

Depositor

Cliff Tabin

Article

Marigo et al Genomics. 1995 Jul 1. 28(1):44-51.

Insert

Ihh (IHH Human)

Expression

Bacterial

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pCEP-WAF1-AS

Plasmid

#16448

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

WAF1 antisense (CDKN1A Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pCEP-WAF1

Plasmid

#16450

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

WAF1 (CDKN1A Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-CKIIa-stGtACR1-FusionRed

Plasmid

#105679

Purpose

Expresses a soma-targeted GtACR1 in mammalian neurons under the CaMKIIa promoter

Depositor

Ofer Yizhar

Article

Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

Insert

stGtACR1

Use

AAV

Tags

FusionRed and Kv2.1

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1-SIO-stGtACR2-FusionRed

Plasmid

#105677

Purpose

Expresses a soma-targeted GtACR2 in Cre-expressing neurons

Depositor

Ofer Yizhar

Article

Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

Has Service

AAV Retrograde, AAV1, and AAV5

Insert

stGtACR2

Use

AAV

Tags

FusionRed and Kv2.1

Expression

Mammalian

Promoter

hSyn1

Available Since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CKIIa-stGtACR2-FusionRed

Plasmid

#105669

Purpose

Expresses a soma-targeted GtACR2 in mammalian neurons under the CaMKIIa promoter

Depositor

Ofer Yizhar

Article

Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

Has Service

AAV Retrograde and AAV1

Insert

stGtACR2

Use

AAV

Tags

FusionRed and Kv2.1

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1-SIO-stGtACR1-FusionRed

Plasmid

#105678

Purpose

Expresses a soma-targeted GtACR1 in Cre-expressing neurons

Depositor

Ofer Yizhar

Article

Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

Has Service

AAV5

Insert

stGtACR1

Use

AAV

Tags

FusionRed and Kv2.1

Expression

Mammalian

Promoter

hSyn1

Available Since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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v1em-Nterm-PE2max-U6-sgRNA

Plasmid

#198732

Purpose

AAV genome encoding N-terminal PE2max and U6 expression cassette

Depositor

David Liu

Article

Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

Insert

Nterm PE2max-NpuN

Use

AAV

Promoter

EFS

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-Nme2ABE8e

Plasmid

#189928

Purpose

Plasmid encoding Nme2ABE8e driven by the CMV promoter for plasmid transfection.

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

Nme2ABE8e

Tags

NLS

Expression

Mammalian

Mutation

Nme2Cas9 D16A

Promoter

CMV

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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