We narrowed to 18,759 results for: nls
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Plasmid#48138PurposeCas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNADepositorInserthSpCas9
UseCRISPRTags3XFLAG and GFPExpressionMammalianPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJM573 ZF6x6-C 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA in TUPV2
Plasmid#161560PurposeInducible expression of 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA under the ZF6x6-C promoterDepositorInsert3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA
UseSynthetic BiologyTags3x-FLAG and HAExpressionMammalianPromoterZF6x6-CAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM576 ZF2x6-C 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA in TUPV2
Plasmid#161562PurposeInducible expression of 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA under the ZF2x6-C promoterDepositorInsert3xFLAG-NLS-VP64-intC-ZF1-NLS-HA
UseSynthetic BiologyTags3x-FLAG and HAExpressionMammalianPromoterZF2x6-CAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM524 EF1α 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA in TUPV2
Plasmid#161553PurposeConstitutive expression of 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA under the EF1α promoterDepositorInsert3xFLAG-NLS-VP64-intC-ZF1-NLS-HA
UseSynthetic BiologyTags3x-FLAG and HAExpressionMammalianPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM525 EF1α 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA in TUPV2
Plasmid#161554PurposeConstitutive expression of 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA under the EF1α promoterDepositorInsert3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA
UseSynthetic BiologyTags3x-FLAG and HAExpressionMammalianPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
AsCas12a-6xNLS-E174R/S542R (pRG232)
Plasmid#149723PurposeLentiviral expression plasmid of AsCas12a-6xNLS-E174R/S542R with puromycin resistance geneDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterEFSAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-TRE3G-FNLS-PGK-Puro
Plasmid#110847PurposeLentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized)DepositorInsertBE3RA-FNLS
UseLentiviralTags3X FLAGMutationD10A and NLS sequence at the N-terminusPromoterTRE3GAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-FNLS-P2A-GFP-PGK-Puro
Plasmid#110869PurposeLentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)DepositorInsertBE3RA-FNLS
UseLentiviralTags3X FLAGMutationD10A and NLS sequence at the N-terminusPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHsp83-NLS-HA-2xPCP-2xGFP
Plasmid#71243PurposeUsed to label reporter mRNAs. Drosophila expression of the PCP protein fused to EGFP.DepositorInsert2xPCP-2xGFP
UseP element-mediated germline transformationTagsEGFPExpressionInsectPromoterHsp83Available sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSpCas9(BB)-2A-Puro (PX459) V2.0
Plasmid#62988PurposeCas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)DepositorInserthSpCas9-2A-Puro V2.0
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianPromoterCbhAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV_BiCHATe27_dTomato_nlsdTomato
Plasmid#213829PurposeAAV construct expressing dTomato driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_dTomato_nlsdTomatoDepositorHas serviceAAV1 and AAV PHP.eBInsertdTomato_P2A_nls.dTomato
UseAAVAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag
Plasmid#132403Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1059AAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
YIPlac204TC-NLS-DsRed-Express2
Plasmid#25443DepositorInsertNLS-DsRed-Express2
TagsNLSExpressionYeastAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1 actin-3XNLS P2A mCherry
Plasmid#58475PurposeExpresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsTagsmCherryExpressionMammalianPromoterCMVAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE
Plasmid#121966PurposeExpresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.DepositorInsertscFv-GCN4
UseLentiviralTagssuperfold GFP-GB1-NLS-iLIDExpressionMammalianPromoterSFFVAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2
Plasmid#170273PurposeNLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2
ExpressionMammalianMutationSee CommentsPromoterCMVd1Available sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG
Plasmid#155305PurposeLentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.DepositorInsertmCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d
UseLentiviralTags2xNLS-3xFLAGExpressionMammalianPromoterEF1aAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPBbsr2-EKARrEV-NLS
Plasmid#173855PurposeEncoding EKARrEV-NLS.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEKARrEV-NLS
ExpressionMammalianAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BII-ChPtW-iRFP670
Plasmid#133397PurposePiggybac vector for constitutive expression of iRFP670-NLSDepositorInsertiRFP-670-NLS
ExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1 R62D actin-3XNLS P2A mCherry
Plasmid#58477PurposeExpresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsTagsmCherryExpressionMammalianMutationChanged Arginine 62 to Aspartic AcidPromoterCMVAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by