We narrowed to 6,175 results for: nls

1. pHR_EF1a-->GssmycLaG16LaG2_P2A_NLSIFP

   Plasmid

   #162228

   Purpose

   Lentiviral vector for the expression of Myc GFP nanbody LaG16LaG2 with IFP NLS in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2020 Oct 16;370(6514):327-331.

   Insert

   LaG16LaG2 and IFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-Bxb1o_nlsFlag

   Plasmid

   #119901

   Purpose

   Expresses Bxb1 integrase in mammalian cells

   Depositor

   Orion Weiner

   Article

   Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

   Insert

   Mammalian codon optimized Bxb1 integrase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. L_NLS 41kDa

   Plasmid

   #201342

   Purpose

   Nucleocytoplasmic transport probe

   Depositor

   Pere Roca-Cusachs

   Article

   Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

   Insert

   (NLS)SV40A4-EGFP-2PrA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. nls-NgAgo-GK

   Plasmid

   #78253

   Purpose

   Mammalian expression of NgAgo (Argonaute from N. gregoryi).

   Depositor

   Chunyu Han

   Article

   Gao et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   NgAgo
   

   Use

   Tags

   a nuclear localization signal (NLS)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. p16 nlsGPS2

   Plasmid

   #228093

   Purpose

   Expression plasmid for genetically-encoded FRET biosensor for Gibberellin (GA)

   Depositor

   Alexander Jones

   Article

   Griffiths et al Plant Cell. 2024 Jul 23:koae198. doi: 10

   Insert

   nlsGPS2
   

   Use

   Tags

   6xHis-T7 XpressTag and cMyc

   Expression

   Plant

   Mutation

   Promoter

   p16

   Available Since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBPnlsLexA::p65Uw

   Plasmid

   #26230

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   nlsLexA::p65 (RELA Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. 4xNLS-pMJ915v2

   Plasmid

   #88917

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNLS-iRFP720

   Plasmid

   #45467

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP720
   

   Use

   Tags

   nuclear localization signal

   Expression

   Mammalian

   Mutation

   T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

   Promoter

   CMV

   Available Since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. 3xnls-mTurquoise2

   Plasmid

   #98817

   Purpose

   In vivo visualization of the nucleus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   3xNLS
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. AsCpf1-2NLS

    Plasmid

    #102565

    Purpose

    bacterial expression of AsCpf1-2NLS

    Depositor

    Jennifer Doudna

    Article

    Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

    Insert

    AsCpf1
    

    Use

    Tags

    6xHis-MBP-TEV and HA-2xSV40NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. M_NLS 41kDa

    Plasmid

    #201341

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    (NLS)SV40A5-EGFP-2PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-21a_FnCpf1_2xNLS_protein_expression

    Plasmid

    #124548

    Purpose

    Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    FnCpf1_2xNLS
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-NLS-HA-B3

    Plasmid

    #51270

    Purpose

    Expression of the site-specific recombinase B3 (mouse codon-optimized)

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    NLS-HA-B3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNLS-2xBFP(4C)

    Plasmid

    #176151

    Purpose

    Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.

    Depositor

    Siegfried Musser

    Article

    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

    Insert

    NLS-2xBFP(4C)
    

    Use

    Tags

    6xHis tag and SV40 nuclear localization sequence …

    Expression

    Bacterial

    Mutation

    Compared with EGFP, the two BFP domains have the …

    Promoter

    Ptrc

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.CBA.loxP.ArcLightD.2A.nlsmCherry.loxP.WPRE.SV40

    Plasmid

    #100030

    Purpose

    AAV expression of ArcLightD

    Depositor

    Lawrence Cohen

    Article

    Storace et al Sci Rep. 2015 May 13;5:10212. doi: 10.10

    Insert

    ArcLightD
    

    Use

    AAV

    Tags

    2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CBA

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. p16 nlsGPS1

    Plasmid

    #101081

    Purpose

    GA biosensor expression plasmid

    Depositor

    Wolf Frommer, Alexander Jones

    Article

    Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

    Insert

    nlsGPS1
    

    Use

    Tags

    cMyc

    Expression

    Plant

    Mutation

    Promoter

    p16 (AT3G60245)

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. L_NLS 67kDa

    Plasmid

    #201361

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    (NLS)SV40A4-EGFP-6PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. H_NLS 67kDa

    Plasmid

    #201372

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    (NLS)SV40-EGFP-6PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV_Efs_hSpCas9_NLS_FLAG-SV40

    Plasmid

    #97307

    Purpose

    AAV vector for encoding a human codon-optimized SpCas9 driven by EFs promoter

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    humanized S. pyogenes Cas9
    

    Use

    AAV and Mouse Targeting

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSynapsin_NLS_GFP-P2A_SNAP_GluA2

    Plasmid

    #170455

    Purpose

    Expresses SNAP tagged GluA2 in neuronal cells

    Depositor

    Shigeki Watanabe

    Article

    Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

    Insert

    GluA2 (Gria2 Mouse)
    

    Use

    Lentiviral

    Tags

    SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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