We narrowed to 6,273 results for: nls

Showing: 421 - 440 of 6273 results

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pBPnlsLexA::p65Uw

Plasmid

#26230

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

nlsLexA::p65 (RELA Human)

Expression

Insect

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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4xNLS-pMJ915v2

Plasmid

#88917

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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3xnls-mScarlet-I

Plasmid

#98816

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

3xNLS

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV_Cas9–superNLS

Plasmid

#232430

Purpose

Expression plasmid for the SpCas9 nuclease with superNLS

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

Cas9–superNLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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p16 nlsGPS2

Plasmid

#228093

Purpose

Expression plasmid for genetically-encoded FRET biosensor for Gibberellin (GA)

Depositor

Alexander Jones

Article

Griffiths et al Plant Cell. 2024 Jul 23:koae198. doi: 10

Insert

nlsGPS2

Tags

6xHis-T7 XpressTag and cMyc

Expression

Plant

Promoter

p16

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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LbCpf1-2NLS

Plasmid

#102566

Purpose

bacterial expression of LbCpf1-2NLS

Depositor

Jennifer Doudna

Article

Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

Insert

LbCpf1

Tags

6xHis-MBP-TEV and HA-2xSV40NLS

Expression

Bacterial

Promoter

T7

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pNLS-iRFP670

Plasmid

#45466

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP670

Tags

nuclear localization signal

Expression

Mammalian

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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p6XHis_NLS-SaCas9

Plasmid

#101086

Purpose

Express SaCas9 in bacteria with a 6xHis tag for purification

Depositor

Rick Tarleton

Article

Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

Insert

CRISPR-associated protein Cas9/Csn1

Use

CRISPR

Tags

TrxA-6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pNLS-iRFP713

Plasmid

#45468

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP713

Tags

nuclear localization signal

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E193K, F198…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pHR_TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP

Plasmid

#162233

Purpose

Lentiviral vector for the expression of GFP nanobody LaG16LaG2 -IFP : TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

anti-GFP nanobody lag16lag2 and IFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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nls-NgAgo-GK

Plasmid

#78253

Purpose

Mammalian expression of NgAgo (Argonaute from N. gregoryi).

Depositor

Chunyu Han

Article

Gao et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

NgAgo

Tags

a nuclear localization signal (NLS)

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2016

Availability

Academic Institutions and Nonprofits only

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pTRIPZ (M)-HT-NLS

Plasmid

#82518

Purpose

Lentivirus vector. Expresses Halotag proteins with nuclear localization signal in mammalian cells.

Depositor

Xiaojun Ren

Insert

Nuclear localizaition sequence

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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Construct 12 - hUBCp_Cas9_3xNLS_p2a_puroR

Plasmid

#81251

Purpose

Lentiviral construct for building stable cell lines expressing Cas9 via puromycin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Cas9-3xNLS

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pGPTVII-nlsSED1

Plasmid

#178190

Purpose

SED1 biosensor for expression in Arabidopsis thaliana

Depositor

Cesar Cuevas-Velazquez, Jose Dinneny

Article

Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

Insert

SED1 biosensor

Tags

SV40 NLS

Expression

Plant

Promoter

pUBQ10

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-P4:gs:nls:CIBN

Plasmid

#135986

Purpose

Expresses the P4 coiled coil fused to the CIBN blue light-inducible domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

P4 coiled-coil fused to the CIBN domain

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pHR'CMVGFP_NLS_hRIF1(1-1308)

Plasmid

#23134

Depositor

Lifeng Xu

Article

Xu et al J Cell Biol. 2004 Dec 6. 167(5):819-30.

Insert

RIF1 deletion construct (RIF1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pNLS-iRFP720

Plasmid

#45467

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Tags

nuclear localization signal

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->GssmycLaG16LaG2_P2A_NLSIFP

Plasmid

#162228

Purpose

Lentiviral vector for the expression of Myc GFP nanbody LaG16LaG2 with IFP NLS in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

LaG16LaG2 and IFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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3xnls-mTurquoise2

Plasmid

#98817

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

3xNLS

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pWPT-nlsLacZ

Plasmid

#12261

Depositor

Didier Trono

Insert

LacZ

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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