We narrowed to 10,863 results for: HA

1. pCVL Traffic Light Reporter 2.1 (VF2468 ZFN target) Ef1a

   Plasmid

   #31483

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Traffic Light Reporter 2.1 (V2468 zfn target) EF Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Traffic Light 2.0 series have a +3 PEST sequence …

   Promoter

   Available Since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEX.d14GFP.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

   Plasmid

   #45564

   Purpose

   Expresses I-Sce I nuclease and BFP tracker in mammalian cells, codes for eGFP donor sequence

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Inserts

   d14 eGFP donor
   I-Sce I Nuclease
   BFP
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deleted n' 15 amino acids

   Promoter

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. p6592 MSCV-IP N-HAonly WWP2

   Plasmid

   #34905

   Depositor

   Peter Howley

   Article

   White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

   Insert

   WWP2 (WWP2 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV LTR

   Available Since

   Feb. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pcS-E626-C1C2

   Plasmid

   #200162

   Purpose

   This plasmid encodes EGFR targeting monobody (E626) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.

   Depositor

   Masako Harada

   Article

   Komuro et al Bioengineering (Basel). 2022 Jan 29;9(2)

   Inserts

   E626
   C1C2 domain of lactadherin
   

   Use

   Tags

   HA and HIS

   Expression

   Mammalian

   Mutation

   sequence adopted from doi: 10.1371/journal.pone.0…

   Promoter

   CMV

   Available Since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSH1/S-Fvls-p30Casp9-E

   Plasmid

   #15272

   Depositor

   David Spencer

   Article

   Fan et al Hum Gene Ther. 1999 Sep 20. 10(14):2273-

   Insert

   FKBP12(V36)-p30Caspase9 (CASP9 Human)
   

   Use

   Tags

   HA epitope and Myristoylation-targeting domain c-…

   Expression

   Mammalian

   Mutation

   FKBP12 F36 to VTruncated Caspase9 (135-416; lacki…

   Promoter

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBSFI-myr-akt-T308A/S473A

   Plasmid

   #49191

   Purpose

   cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 and S to A at position 473 in cloning vector

   Depositor

   Peter Vogt

   Article

   Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Adaptor plasmid

   Tags

   HA tag and myristolation signal

   Expression

   Mutation

   T308A; S473A

   Promoter

   T7

   Available Since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pM125: pAAV-EFS-CasRx-VEGFA array presgRNA

   Plasmid

   #166873

   Purpose

   AAV vector for expressing CasRx and human VEGFA targeting presgRNA array for RNA-editing

   Depositor

   Guei-Sheung Liu

   Article

   Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

   Inserts

   U6-VEGFA array presgRNA
   RfxCas13d
   

   Use

   AAV and CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP

   Plasmid

   #45579

   Purpose

   Expresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP tracker

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Insert

   coY2 Ani K227M
   

   Use

   Lentiviral

   Tags

   HA, IRES BFP, and NLS

   Expression

   Mammalian

   Mutation

   co indicates codon optimization for mammalian exp…

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG_EGFP-RandE3

   Plasmid

   #79870

   Purpose

   Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-RandE3
   

   Use

   Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Beta-actin

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCGN N-Ras 12D

    Plasmid

    #14725

    Depositor

    Adrienne Cox

    Article

    Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

    Insert

    N-ras (NRAS Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    12D

    Promoter

    Available Since

    Aug. 29, 2007

    Availability

    Academic Institutions and Nonprofits only

11. 1051 pBabe puroL Akt

    Plasmid

    #9011

    Depositor

    William Sellers

    Article

    DiRenzo et al Cancer Res. 2002 Jan 1. 62(1):89-98.

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

12. pYX233

    Plasmid

    #163762

    Purpose

    Empty vector for Gal-inducible yeast expression

    Depositor

    Johannes Herrmann

    Article

    Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

    Type

    Empty backbone

    Use

    Tags

    Hemagglutinin (HA) tag

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCR1306

    Plasmid

    #111128

    Purpose

    Expresses FANCE.

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCE (FANCE Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Nrxn3α-AS4-

    Plasmid

    #184332

    Purpose

    Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

    Depositor

    Peter Scheiffele

    Article

    Nguyen et al Elife. 2016 Dec 13;5. doi: 10.7554/eLife

    Insert

    Nrxn3-alpha (Nrxn3 Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Beta-Globin-PTC39

    Plasmid

    #130701

    Purpose

    Expresses Beta-globin-PTC39 NMD reporter (It has a Premature termination codon (PTC) at 39th Amino acid)

    Depositor

    Marvin Tanenbaum

    Article

    Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

    Insert

    Beta Globin (HBB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PTC39

    Promoter

    Pcmv

    Available Since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV E2F1 E132

    Plasmid

    #24224

    Purpose

    Mammalian expression of human E2F1 (DNA binding mutant) with HA tag

    Depositor

    Kristian Helin

    Article

    Helin et al Genes Dev. 1993 Oct . 7(10):1850-61.

    Insert

    E2F1 E132 (E2F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

17. p6614 MSCV-IP N-HAonly PXMP3

    Plasmid

    #35012

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    PXMP3 (PEX2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available Since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

18. scFv-GCN4-DNMT3a-DNMT3l

    Plasmid

    #154140

    Purpose

    Expresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
    

    Use

    Tags

    HA and sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR LAT-FFF/Zap70 iLID-Drop

    Plasmid

    #171032

    Purpose

    Light-induced LAT-Zap70 dimerization and LAT clustering. Zap70 is TagRFP-tagged. LAT has three Tyr-Phe mutations.

    Depositor

    Jared Toettcher

    Article

    Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

    Insert

    LAT (LAT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-hSHH

    Plasmid

    #179586

    Purpose

    ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette with SHH expression (Red module-dsRed/Green module-NeonGreen T2A SHH)

    Depositor

    Ali Hemmati Brivanlou

    Article

    De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

    Insert

    NLS-NG-T2A-hSHH
    

    Use

    Piggybac

    Tags

    NeonGreen

    Expression

    Mutation

    Promoter

    TRE

    Available Since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAC91-pmax-dCas9VP64

    Plasmid

    #48223

    Purpose

    dCas9VP64 on pmax expression vector

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP64 activation domain
    

    Use

    CRISPR

    Tags

    HA Tag and VP64

    Expression

    Mammalian

    Mutation

    D10A;H840A (catalytically inactive)

    Promoter

    CAGGS

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pQCXIP TBRII

    Plasmid

    #19147

    Depositor

    Joan Massague

    Article

    Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

    Insert

    Transforming growth factor, beta receptor II (TGFBR2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

23. Pinco-mNrp1

    Plasmid

    #21937

    Depositor

    Guido Serini

    Article

    Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

    Insert

    neuropilin 1 (Nrp1 Mouse)
    

    Use

    Retroviral

    Tags

    HA and His

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

24. pSH1/M-Fv-Casp3-E

    Plasmid

    #15271

    Depositor

    David Spencer

    Article

    Fan et al Hum Gene Ther. 1999 Sep 20. 10(14):2273-

    Insert

    FKBP12(V36)-Caspase3 (CASP3 Human)
    

    Use

    Tags

    HA epitope and Myristoylation-targeting domain c-…

    Expression

    Mammalian

    Mutation

    FKBP12(V36) F36->V

    Promoter

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCD564

    Plasmid

    #153026

    Purpose

    dxCas9(3.7), MCP-SoxS(R93A/S101A)

    Depositor

    Jesse Zalatan

    Article

    Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

    Insert

    dxCas9(3.7), MCP-SoxS(R93A/S101A)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Promoter

    Available Since

    Aug. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCH62 (denAsCas12a-KRAB piggyBac)

    Plasmid

    #217333

    Purpose

    piggyBac expression of denAsCas12a-KRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    denAsCas12a
    

    Use

    CRISPR

    Tags

    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    D908A/E174R/S542R/K548R

    Promoter

    CAG

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CRY2olig-mCh-Nck

    Plasmid

    #60034

    Purpose

    Expresses a fusion containing CRY2olig (PHR, E490G) with mCherry and 3 SH3 domains of Nck

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

    Insert

    CRY2-mCh-NCK (CRY2 Mustard Weed, Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    M354I, E490G CRY2

    Promoter

    CMV

    Available Since

    Nov. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pACT2-μ3A

    Plasmid

    #198174

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP-3 μ3A
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    silent substitution in codon 225

    Promoter

    ADH1

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p6641 MSCV-P C-FlagHA 18E7-Kozak

    Plasmid

    #35019

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    HPV18 E7 (E7 HPV 18)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available Since

    March 14, 2012

    Availability

    Academic Institutions and Nonprofits only

30. p6642 MSCV-P C-FlagHA 6bE7-Kozak

    Plasmid

    #35020

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    HPV6b E7 (E7 HPV 6b)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available Since

    March 6, 2012

    Availability

    Academic Institutions and Nonprofits only

31. Cerulean-GalT

    Plasmid

    #11930

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Cole et al Science. 1996 Aug 9. 273(5276):797-801.

    Insert

    GalT (GALT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Amino acids 1-60 of the human galactosyltransfera…

    Promoter

    Available Since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV5 MEKK4alpha WT

    Plasmid

    #12187

    Depositor

    Gary Johnson

    Article

    Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-9

    Insert

    MEKK4 (Map3k4 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

33. Nrxn3α-AS4+

    Plasmid

    #184333

    Purpose

    Expression of mouse Nrx3-alpha containing insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

    Depositor

    Peter Scheiffele

    Article

    Nguyen et al Elife. 2016 Dec 13;5. doi: 10.7554/eLife

    Insert

    Nrxn3-alpha (Nrxn3 Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. SB51

    Plasmid

    #48184

    Purpose

    Express full length HA tagged p35sr in mammalian cells

    Depositor

    Shoumo Bhattacharya

    Article

    Bhattacharya et al Genes Dev. 1999 Jan 1. 13(1):64-75.

    Insert

    Cited2 (CITED2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

35. p6640 MSCV-P C-FlagHA 16E7-Kozak

    Plasmid

    #35018

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    HPV16 E7 (E7 HPV 16)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available Since

    March 14, 2012

    Availability

    Academic Institutions and Nonprofits only

36. pSH1/Mf-del-Akt-E

    Plasmid

    #15288

    Depositor

    David Spencer

    Article

    Park et al Nat Biotechnol. 2006 Dec . 24(12):1581-9

    Insert

    delPH-Akt (Akt1 Mouse)
    

    Use

    Tags

    HA epitope and Myristoylation-targeting domain Fy…

    Expression

    Mammalian

    Mutation

    Truncated PH (residues 1-99)

    Promoter

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. LPCX BMP-RIB Q203D

    Plasmid

    #12642

    Depositor

    Rik Derynck

    Article

    Skillington et al J Cell Biol. 2002 Oct 14. 159(1):135-46.

    Insert

    BMP-RIB (Bmpr1b Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q203D, activating mutation

    Promoter

    Available Since

    Oct. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

38. PKG I alpha dNPS

    Plasmid

    #16391

    Depositor

    Bernard Weinstein

    Article

    Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

    Insert

    PKG1 alpha (PRKG1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N-terminal pseudosubstrate domain deletion: delet…

    Promoter

    Available Since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

39. pSLIK-Hygro-Myc-hAgo2

    Plasmid

    #60234

    Purpose

    Express HA-tagged wild-type Ago2 in mammalian cells in a Dox-inducible manner

    Depositor

    Zissimos Mourelatos

    Article

    Liu et al Mol Cell. 2012 May 25;46(4):507-17. doi:

    Insert

    Argonaute2 (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

40. c3GIC9n

    Plasmid

    #62192

    Purpose

    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes

    Depositor

    Lukas Dow

    Article

    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

    Insert

    humanized S. Pyogenes Cas9 (D10A)
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mutation

    D10A substitution (Cas9n)

    Promoter

    TRE3G

    Available Since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge