We narrowed to 83,287 results for: Mycs

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pAJF1124

Plasmid

#252904

Purpose

3' rpoC(MSMEG_1368)-ALFA for rpoC-ALFA in M. smegmatis via single crossover. Encodes hygromycin-resistance cassette.

Depositor

Michael Glickman

Article

Fay et al mBio. 2025 Aug 13;16(8):e0097125. doi: 1

Insert

3' rpoC(MSMEG_1368)-ALFA

Tags

ALFA tag

Expression

Bacterial

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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p2OT-MtbTOP1

Plasmid

#250351

Purpose

Expresses Mycobacterium tuberculosis topoisomerase I protein with N-terminal His6-Mocr tag

Depositor

Yuk-Ching Tse-Dinh

Article

Tan et al J Mol Biol. 2016 Jan 16;428(1):182-193.

Insert

Mycobacterium tuberculosis topoisomerase I

Tags

6xHis mocr

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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p2OT-MtbTOP1-704t

Plasmid

#250352

Purpose

Expresses truncated Mycobacterium tuberculosis topoisomerase I with N-terminal His6-Mocr tag

Depositor

Yuk-Ching Tse-Dinh

Article

Tan et al J Mol Biol. 2016 Jan 16;428(1):182-193.

Insert

Mycobacterium tuberculosis topoisomerase I residues 2-704

Tags

6xHis mocr

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-BleMX6

Plasmid

#240974

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; BleMX6 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

BleMX6

Expression

Bacterial

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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Hygro-Bem1-mCherry-AID

Plasmid

#173925

Purpose

Plasmid designed for the genomic tagging of saccharomyces cerevisiae Bem1 with mCherry-AID

Depositor

Liedwij Laan

Article

Daalman et al Philos Trans R Soc Lond B Biol Sci. 2023

Inserts

Partial Bem1 C-terminus
hygromycin marker
Bem1 downstream sequence

Tags

mCherry- AID

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1021-Tier1-PhCMV-PuroR

Plasmid

#169492

Purpose

Tier-1 vector encoding PhCMV-driven marker for puromycin selection (PhCMV-PuroR-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven puromycin N-acetyl-transferase

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFPJ1

Plasmid

#83561

Purpose

Express yEGFP-ERG9 ER signal peptide under the control of TEF1 promoter

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

Insert

TEF1 promoter-yEGFP-ERG9 ER signal peptide

Expression

Yeast

Promoter

TEF1

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pRosetta_v2

Plasmid

#136477

Purpose

Lentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycin

Depositor

John Doench

Article

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

HygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO mouse shRNA 1 rictor

Plasmid

#21341

Depositor

David Sabatini

Article

Thoreen et al J Biol Chem. 2009 Mar 20. 284(12):8023-3

Insert

rictor shRNA 1 (Rictor Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 6, 2009

Availability

Academic Institutions and Nonprofits only

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pLKO mouse shRNA 1 raptor

Plasmid

#21339

Depositor

David Sabatini

Article

Thoreen et al J Biol Chem. 2009 Mar 20. 284(12):8023-3

Insert

raptor shRNA 1 (Rptor Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 7, 2009

Availability

Academic Institutions and Nonprofits only
pSH231-EF1-Puro

Plasmid

#115143

Purpose

Safe harbor site 231 knock-in vector with puromycin resistance selection cassette

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

Puromycin

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pUDP293

Plasmid

#204228

Purpose

Episomal yeast plasmid expressing the Ercas12a and ErCas12a gRNA

Depositor

Jean-Marc Daran

Article

Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

Inserts

Eubacterium rectale cas12a (Ercas12a)
BsaI_GFP drop-out_BsaI

Use

Synthetic Biology

Tags

NLS

Mutation

codon optimized for S. cerevisiae

Promoter

Saccharomyces cerevisiae PGK1 and Saccharomyces c…

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBC155

Plasmid

#202274

Purpose

Level 2 for strain-specific barcode DNA tag with bc182 through Tn7 bacterial insertion, Spectinomycin and Streptomycin selectable marker and tagBFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc182

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pNIC28-Bsa4-ICL1

Plasmid

#105822

Purpose

Expression of Mycobacterium tuberculosis icl1 recombinant protein

Depositor

Ivanhoe Leung

Article

Bhusal et al Medchemcomm. 2017 Oct 17;8(11):2155-2163

Insert

icl1

Tags

His tag with TEV cleavage

Expression

Bacterial

Promoter

T7

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pILGFPU0 (R)

Plasmid

#83562

Purpose

Express yEGFP-ERG9 ER targeting peptide-CLN2PEST under the control of TEF1 promoter

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

Insert

TEF1 promoter-yEGFP-ERG9 ER targeting peptide-CLN2PEST

Expression

Yeast

Promoter

TEF1

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pHYG

Plasmid

#243678

Purpose

Expresses the HYGROMYCIN PHOSPHOTRANSFERASE (HPT) CDS in plants using the Cestrum Yellow Leaf Curling Virus (CmYLCV) 9.11 promoter and an Arabidopsis HSP terminator

Depositor

Patrick Krysan

Article

De La Mora et al Plant Cell Rep. 2025 Oct 19;44(11):243.

Insert

HYGROMYCIN PHOSPHOTRANSFERASE (HPT) CDS

Expression

Plant

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEM.H10L

Plasmid

#198683

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XI1_UP

Use

Synthetic Biology

Expression

Yeast

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.H10R

Plasmid

#198684

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XI1_DW

Use

Synthetic Biology

Expression

Yeast

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.H09R

Plasmid

#198681

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XII4_UP

Use

Synthetic Biology

Expression

Yeast

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.H09L

Plasmid

#198682

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XII4_DW

Use

Synthetic Biology

Expression

Yeast

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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