We narrowed to 5,494 results for: SPI

1. pAAV-hSyn-sbGLuc-CheRiff-EGFP

   Plasmid

   #114108

   Purpose

   fusion protein of Gaussia luciferase variant slow burn, synthetic channelrhodopsin CheRiff, and enhanced green fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Ikefuama et al Int J Mol Sci. 2022 Oct 27;23(21):12994.

   Insert

   Gaussia luciferase, slow burn; CheRiff synthetic ChR; EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. MBP-GTSE1(long)-His6

   Plasmid

   #69110

   Purpose

   For bacterial expression of recombinant human GTSE1 (longer isoform) tagged with MBP and His.

   Depositor

   Stephen Royle

   Article

   Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

   Insert

   G2- and S-phase Expressed 1 (GTSE1 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGY416

   Plasmid

   #166660

   Purpose

   For light-inducible Cre recombinase (LiCre) expression under the Gal1 promoter in yeast

   Depositor

   Gaël Yvert

   Article

   Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

   Insert

   LiCre
   

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Cre: E340A, D341A

   Promoter

   GAL1

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti6.2-coHPDL WT 3xFlag-V5

   Plasmid

   #174129

   Purpose

   Expresses codon-optimized HPDL with a 3xFlag-V5 tag in mammalian cells

   Depositor

   Michael Pacold

   Article

   Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

   Insert

   4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG-V5

   Expression

   Mammalian

   Mutation

   Codon optimized

   Promoter

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Topo_SpCas9.tracr_LbCas12a.DR

   Plasmid

   #155049

   Purpose

   TOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   SpCas9 tracrRNA and LbCas12a Direct Repeat (DR)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pST_MAHS_RAMVA_81-236-FLAG (pBS0297)

   Plasmid

   #185328

   Purpose

   For the mammalian expression of the tardigrade protein MAHS_RAMVA_81-236-FLAG. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   MAHS_RAMVA_81-236-FLAG
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. CRY-tTA (B702)

   Plasmid

   #92032

   Purpose

   Expresses CRY2 (Full length, mutated NLS) fusion with tTA2 'tet-OFF' transcriptional activator

   Depositor

   Chandra Tucker

   Article

   Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   tTA2 (TetR fused to VP64)

   Expression

   Mammalian

   Mutation

   NLS on CRY2 is mutated

   Promoter

   CMV

   Available since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-PH-SWAP70(R223E, R224E)

   Plasmid

   #84583

   Purpose

   Expresses GFP-tagged PH-domain of SWAP70 in mammalian cells, impaired phosphoinositide binding

   Depositor

   Geert van den Bogaart

   Article

   Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do

   Insert

   SWAP70 residues 209-306 with mutations R223E and R224E (SWAP70 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Pleckstrin-homology domain, residues 209-306, mut…

   Promoter

   CMV

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pGY466

   Plasmid

   #166661

   Purpose

   For light-inducible Cre recombinase (LiCre) expression under the Met17 promoter in yeast

   Depositor

   Gaël Yvert

   Article

   Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

   Insert

   LiCre
   

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Cre: E340A, D341A

   Promoter

   MET17

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-α-COPI-WD40

    Plasmid

    #180758

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    α-COPI-WD40
    

    Use

    Tags

    C-terminal strep tag

    Expression

    Mammalian

    Mutation

    Encodes residues 1-327; mutations: Leu181Lys, Leu…

    Promoter

    CMV

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-C1-MKLP1 S708/710A

    Plasmid

    #70153

    Purpose

    mammalian expression of MKLP1 with an eGFP fusion and S708A/S710A mutations

    Depositor

    Masanori Mishima

    Article

    Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

    Insert

    MKLP1 (KIF23 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S708A, S710A

    Promoter

    CMV

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX4T-1 hSlp4-a del.SHD

    Plasmid

    #40067

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp4-a V21A (SYTL4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deleted SHD domain (1-143)

    Promoter

    Tac

    Available since

    Oct. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pEF1a-CRY2PHR-SID4X

    Plasmid

    #63663

    Purpose

    AAV expression of CRYS2PHR-SID4X

    Depositor

    Dong Kong

    Article

    Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

    Insert

    SID4X-CYR2PHR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C1-MKLP1 S708A

    Plasmid

    #70146

    Purpose

    mammalian expression of MKLP1 with an eGFP fusion and an S708A mutation

    Depositor

    Masanori Mishima

    Article

    Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

    Insert

    MKLP1 (KIF23 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S708A

    Promoter

    CMV

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5-FRT/TO-neo-C11orf51-3xFlag (2765)

    Plasmid

    #39859

    Depositor

    Jonathon Pines

    Article

    Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

    Insert

    C11orf51 (ANAPC15 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pbIG-Xph20-ETWV

    Plasmid

    #133034

    Purpose

    Expresses Xph20-ETWV in bacteria

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph20-ETWV
    

    Use

    Tags

    AviTag, His10, and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    S63K

    Promoter

    T7

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX4T-1 hSlp4-a V21A

    Plasmid

    #40064

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp4-a V21A (SYTL4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Valine 21 to Alanine

    Promoter

    Tac

    Available since

    Oct. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pEGFP-C1-MKLP1 S710A

    Plasmid

    #70149

    Purpose

    mammalian expression of MKLP1 with an eGFP fusion and an S710A mutation

    Depositor

    Masanori Mishima

    Article

    Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

    Insert

    MKLP1 (KIF23 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S710A

    Promoter

    CMV

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSUPER-retro-puro-Smad3

    Plasmid

    #89828

    Purpose

    Knockdown human Smad3 expression in human mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

    Insert

    SMAD3 shRNA (SMAD3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. 3' MBD3 TALEN

    Plasmid

    #52359

    Purpose

    TALEN vector for targeting human MBD3 locus (3')

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    NN NN HD NN NG NN NI HD HD HD HD NI NN NI HD HD NG
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only