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pcDNA5-FRT-TO-nsp7

Plasmid

#201025

Purpose

Expresses SARS-CoV-2 nsp7 under control of a tetracycline-inducible promoter in mammalian.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Promoter

CMV-tet-ON

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ230-D156R

Plasmid

#188542

Purpose

Gateway entry clone (attL1 & attR5) for LbCas12a-D156R mediated genome editing

Depositor

Yiping Qi

Article

Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

Insert

LbCas12a-D156R

Use

CRISPR; Gateway compatible lbcas12a-d156r entry c…

Tags

NLS

Expression

Plant

Mutation

D156R

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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DsRed -7e

Plasmid

#191765

Purpose

Expression of DsRed fused to a negatively charged polypeptide. Overall charge on tetramer: -7e

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

DsRed

Tags

N terminal fusion of negatively charged polypetod…

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-SARS-CoV-2 5’UTR ΔSL1-Luciferase

Plasmid

#191481

Purpose

Luciferase reporter for SARS-CoV-2 5' UTR ΔSL1

Depositor

Hao Wu

Article

Shi et al bioRxiv. 2020 Sep 18. doi: 10.1101/2020.

Insert

SARS-CoV-2 5' UTR ΔSL1

Use

Lentiviral and Luciferase

Mutation

Missing the stem-loop 1 region of SARS-CoV-2 5&#x…

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 BS (-) N protein

Plasmid

#188448

Purpose

SARS CoV-2 N protein expression

Depositor

Ajit Chande

Article

Mishra et al Life Sci Alliance. 2022 Mar 16;5(7). pii

Insert

SARS-CoV-2 N protein

Tags

Strep-Tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pST_CAHS1_PARRC_150-189 (pBS0658)

Plasmid

#185201

Purpose

For the mammalian expression of the tardigrade protein CAHS1_PARRC_150-189. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS1_PARRC_150-189

Expression

Mammalian

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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ptia1l-hsp-GFP

Plasmid

#177837

Purpose

Donor plasmid for knock-in of hsp70-EGFP using CRISPR-Cas9

Depositor

David Kingsley

Article

Wucherpfennig et al Nat Ecol Evol. 2022 Sep 1. pii: 10.1038/

Insert

tia1l-sgRNA target site-hsp70-EGFP

Use

CRISPR

Tags

EGFP

Promoter

hsp70

Available Since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_A234G (pBS0816)

Plasmid

#185267

Purpose

For the mammalian expression of the human protein APOE_HUMAN_A234G. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_A234G

Expression

Mammalian

Mutation

A234G

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pST_D4NWF2_DrHD_b03_dom3 (pBS0681)

Plasmid

#185207

Purpose

For the mammalian expression of the Deinococcus radiodurans protein D4NWF2_DrHD_b03_dom3. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

D4NWF2_DrHD_b03_dom3

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_AfLEA1.1 (pBS0741)

Plasmid

#185233

Purpose

For the mammalian expression of the brine shrimp protein AfLEA1.1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AfLEA1.1

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_Q19228_CAEEL_51-88 (pBS0734)

Plasmid

#185230

Purpose

For the mammalian expression of the C. elegans protein Q19228_CAEEL_51-88. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Q19228_CAEEL_51-88

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_pvLEA22mer_shuffle_5 (pBS0731)

Plasmid

#185228

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_shuffle_5. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_shuffle_5

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_pvLEA22mer_shuffle_3 (pBS0729)

Plasmid

#185227

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_shuffle_3. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_shuffle_3

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_pvLEA22mer_shuffle_1 (pBS0727)

Plasmid

#185226

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_shuffle_1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_shuffle_1

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_pvLEA22mer_mutant_5 (pBS0722)

Plasmid

#185225

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_5. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_5

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_pvLEA22mer_mutant_3 (pBS0720)

Plasmid

#185224

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_3. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_3

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_pvLEA22mer_mutant_23 (pBS0719)

Plasmid

#185223

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_23. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_23

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_pvLEA22mer_mutant_22 (pBS0718)

Plasmid

#185222

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_22. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_22

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_pvLEA22mer_mutant_21 (pBS0717)

Plasmid

#185221

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_21. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_21

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_pvLEA22mer_mutant_2 (pBS0715)

Plasmid

#185220

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_2. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_2

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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