163,607 results

1. pCDNA5/FRT/TO_HaloTag7_T2A_EGFP

   Plasmid

   #169325

   Purpose

   Expression of HaloTag7 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pQTEV-PSMD11

   Plasmid

   #31333

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 (PSMD11 Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PB-CAG-rtTA-IRES-eGFP-pGK-NeoR

   Plasmid

   #204497

   Purpose

   Expresses rtTA and Neomycin resistance genes in mammalian cells

   Depositor

   Wataru Fujii

   Article

   Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

   Insert

   rtTA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AbVec2.0-mIghg1

   Plasmid

   #127158

   Purpose

   Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG1 isotype

   Depositor

   Hedda Wardemann

   Insert

   Ighg1 (Ighg1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available Since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. miRFP670nano-pcDNA

   Plasmid

   #127443

   Purpose

   Monomeric near-infrared fluorescent protein miRFP670nano in mammalian plasmid

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   miRFP670nano
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTol2CG2-hsp70I:QFGal4-SV40pA

   Plasmid

   #155120

   Purpose

   Tol2 vector for heat-shock inducible expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   hsp70l:QFGal4 (hsp70l Budding Yeast, Neurospora crassa, Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   hsp70l

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV HDM2(C464A)

   Plasmid

   #12086

   Depositor

   Tyler Jacks

   Article

   Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

   Insert

   HDM2 (MDM2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C464A: RING-finger-domain mutant

   Promoter

   Available Since

   June 8, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pELPS-eDHFR-YFP-T2A-Renilla

   Plasmid

   #193253

   Purpose

   Triple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase

   Depositor

   Mark Sellmyer

   Article

   Sellmyer et al Mol Ther. 2020 Jan 8;28(1):42-51. doi: 1

   Insert

   The eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
   

   Use

   Lentiviral

   Tags

   Renilla luciferase (rLuc) and yellow fluorescent …

   Expression

   Mutation

   None

   Promoter

   EF1a

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBHt2G-RFP

   Plasmid

   #107162

   Purpose

   Golden-Gate compatible fungal transformation vector carrying Hygromycin Resistance w RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)

   Depositor

   Sophien Kamoun

   Type

   Empty backbone

   Use

   Fungal grobacterium transformation plasmid, teste…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBS-U6-crRNA-empty

    Plasmid

    #134921

    Purpose

    Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pCAG-All-in-one-hCascade.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    crRNA for CRISPR-Cas3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. attP-puro donor

    Plasmid

    #182139

    Purpose

    attP-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    attP-EGFP-EF1α-PuroR-T2A-TagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHR-flag-GFP-DLL1

    Plasmid

    #205192

    Purpose

    This vector encodes of a synCAM (DLL1) and GFP

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM DLL1 and GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SAC1deltaTMD-FKBP-mCherry

    Plasmid

    #108123

    Purpose

    Recruitable cytosolic SAC1

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    SACM1L(1-520):FKBP1A(3-108):mCherry (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1L(1-520)

    Promoter

    CMV

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-teLuc c-myc

    Plasmid

    #100026

    Purpose

    Enhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)

    Depositor

    Huiwang Ai

    Article

    Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

    Insert

    teLuc, a NanoLuc mutant
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    D19S, D85N, C164H

    Promoter

    CMV

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSFFV_mNG3K(1-10)

    Plasmid

    #157993

    Purpose

    Lentiviral vector of constitutive expression of mNeonGreen3K(1-10)

    Depositor

    Bo Huang

    Article

    Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

    Insert

    pSFFV_mNG3K(1-10)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    G27D, D42E, M128I, K153M, T170I

    Promoter

    SFFV

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. YFP-CL1

    Plasmid

    #11950

    Depositor

    Nico Dantuma

    Article

    Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

    Insert

    YFP-CL1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. G384A_pLenti-TetBattP(GT)-BFP-2A-iCasp9-2A-Blast_rtTA3

    Plasmid

    #200630

    Purpose

    Negative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GT) landing pad

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

    Insert

    iCasp9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    pTRE3G

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EYFP-Annexin-12

    Plasmid

    #56583

    Depositor

    Michael Davidson

    Insert

    Annexin (A4) (ANXA4 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pTT3-SP-6xHis-NTRK1(FL)-FLAG

    Plasmid

    #157620

    Purpose

    Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    NTRK1 (NTRK1 Human)
    

    Use

    Tags

    6xHis and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MADR pDonor-H3F3A-G34R-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid

    #131463

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A G34R, PDGFRA D842V, and TRP53

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    H3F3A-G34R-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    Trp53-V5, H3F3A-EGFP-AU1

    Expression

    Mammalian

    Mutation

    H3F3A G34R, Pdgfra D842V

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available Since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge