We narrowed to 29,518 results for: REP

1. eGFP-KIF5A-Rigor

   Plasmid

   #172202

   Purpose

   Expresses KIF5A G235A rigor mutant tagged with eGFP in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Farias et al Cell Rep. 2015 Nov 10;13(6):1221-1232. d

   Insert

   KIF5A (Kif5a Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Changed Glycine 235 to Alanine

   Promoter

   CMV

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-GSDMD_NoCys-Myc

   Plasmid

   #201169

   Purpose

   Expression of GSDMD gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

   Insert

   Gasdermin D (Gsdmd Mouse)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   C39A, C57A, C77A, C122A, C265A, C192A

   Promoter

   MESV

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3-PKA-RIIβ-sREACh

   Plasmid

   #114113

   Purpose

   Mammalian expression of rat PKA regulatory subunit IIβ C-terminally tagged by sREACh.

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   PKA regulatory subunit RII beta tagged by sREACh (low-irradiant YFP)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV-GSDMD_C192A-Myc

   Plasmid

   #201168

   Purpose

   Expression of GSDMD gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

   Insert

   Gasdermin D (Gsdmd Mouse)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   C192A

   Promoter

   MESV

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR221-TAZ CA

   Plasmid

   #79504

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TAZ S89A mutant CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   WWTR1 (WWTR1 Human)
   

   Use

   Multisite gateway entry clone

   Tags

   3xFLAG

   Expression

   Mutation

   S89A

   Promoter

   no Promoter

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFP

   Plasmid

   #162336

   Purpose

   Lentiviral expression of sgNGN3 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   S. pyogenes sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6; mU6

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-FLEX-SaCas9-U6-sgGabrg1

   Plasmid

   #124856

   Purpose

   Mutagenesis of Gabrg1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gabrg1 (Gabrg1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR3-vsv-GCN4-dCEN-INCENP

   Plasmid

   #108510

   Purpose

   expression of vsv-GCN4-dCEN-INCENP

   Depositor

   Susanne Lens

   Article

   van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

   Insert

   GCN4-dCEN-INCENP (INCENP Human)
   

   Use

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   replace CEN box for GCN4 dimerazation domain

   Promoter

   CMV

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST40-2XHA-Src-S4-UD

   Plasmid

   #140304

   Purpose

   Expresses HA-tagged human Src-S4-UD in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Use

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   contains only the SH4 and UD regions

   Promoter

   CMV

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST40-2XHA-Src-S4-KD

    Plasmid

    #140296

    Purpose

    Expresses HA-tagged human Src-S4-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    contains only the SH4 and KD regions

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST40-2XHA-Src-S32

    Plasmid

    #140308

    Purpose

    Expresses HA-tagged human Src-S32 in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    contains only the SH3 and SH2 domains

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-FLEXfrt-SaCas9-U6-sgGrin2b

    Plasmid

    #124864

    Purpose

    Mutagenesis of Grin2b

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin2b (Grin2b Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP_E1770Q-talin1 (1-2541)

    Plasmid

    #166121

    Purpose

    For expression of full-length mouse talin-1 carrying mutation E1770Q in mammalian cells. Contains C-terminal EGFP tag.

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    E1770Q-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    E1770Q

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNIC28-BSA4-G-S18

    Plasmid

    #127825

    Purpose

    Production of recombinant human ribosomal protein S18

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPS18 (RPS18 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAWp100

    Plasmid

    #157977

    Purpose

    pBT264-mutmU6p-{2xBbsI}-sgRNA scaffold-{MfeI}

    Depositor

    Alan Siu Lun Wong

    Article

    Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGex4t2 with 244-458AA SUUR fragment

    Plasmid

    #112989

    Purpose

    Expresses fusion of GST and amino acids 244-458 of Drosophila SUUR

    Depositor

    Terry Orr-Weaver

    Article

    Nordman et al Cell Rep. 2014 Nov 6;9(3):841-9. doi: 10

    Insert

    SuUR (SuUR Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amino acids 244-458

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLQ1371-sgITGB1-1

    Plasmid

    #121533

    Purpose

    sgITGB1-1 sequence: GAAGCAGGGCCAAATTGTGGG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

    Depositor

    Ovijit Chaudhuri, Stanley Qi

    Article

    Lee et al Sci Rep. 2019 Nov 20;9(1):17188. doi: 10

    Insert

    sgITGB1-1
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLQ1371-sgITGB4-1

    Plasmid

    #121535

    Purpose

    sgITGB4-1 sequence: GAGGCGCAGTCCTTATCCACA. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

    Depositor

    Ovijit Chaudhuri, Stanley Qi

    Article

    Lee et al Sci Rep. 2019 Nov 20;9(1):17188. doi: 10

    Insert

    sgITGB4-1
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST40-2XHA-Src-S32-KD

    Plasmid

    #140310

    Purpose

    Expresses HA-tagged human Src-S32-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    Contains only the SH3, SH2, and KD regions

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAav-MCS-PQS1-3xFLAG

    Plasmid

    #84883

    Purpose

    rAAV-based template for genome engineering of protein C-termini containing PQS1 and 3xFLAG tags and a selection cassette

    Depositor

    Sven Eyckerman

    Article

    Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

    Insert

    Multicloning sites and selection cassette
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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