We narrowed to 12,452 results for: SOM

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pcDNA3.1(+)/CUG-nLuc-3XFLAG-PEST

Plasmid

#127313

Purpose

Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from CUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUU-nLuc-3XFLAG-CL1/PEST

Plasmid

#127319

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUU start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/(CAA)26 5′ leader AUG-nLuc-3XFLAG

Plasmid

#127324

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (CAA)26 repeats in 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GGG-stop-nLuc-3XFLAG

Plasmid

#127311

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from negative control GGG start codon, but translation terminated immediately by stop codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AAA-stop-nLuc-3XFLAG

Plasmid

#127310

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from negative control AAA start codon, but translation terminated immediately by stop codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GGG-nLuc-3XFLAG

Plasmid

#127307

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from GGG start codon (Negative control plasmid)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/BAG1 5′ UTR AUG-nLuc-3xFLAG

Plasmid

#127329

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with BAG1 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/BAG1 5′ UTR CUG-nLuc-3xFLAG

Plasmid

#127328

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon from mRNA with BAG1 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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A3H HapII R175/6E-Cas9n-UGI

Plasmid

#119142

Purpose

Base editor made from APOBEC3H Haplotype II RNA binding mutant RR175/6EE

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3H Haplotype II RR175/6EE-Cas9 nickase-UGI

Use

CRISPR

Mutation

A3H Hap II RR175/6EE

Promoter

CMV

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pET151-GST-PP-RPN13 (19-132) K83A

Plasmid

#125829

Purpose

Expresses GST-RPN13 (19-132) K83A in E.coli

Depositor

Chris Hill

Article

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

changed lysine 83 to alanine

Promoter

T7

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pET151-GST-PP-RPN13 (19-132) K97A

Plasmid

#125823

Purpose

Expresses GST-RPN13 (19-132) K97A in E.coli

Depositor

Chris Hill

Article

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

changed lysine 97 to alanine

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pET151-GST-PP-RPN13 (19-132) K97D

Plasmid

#125824

Purpose

Expresses GST-RPN13 (19-132) K97D in E.coli

Depositor

Chris Hill

Article

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

changed lysine 97 to aspartate

Promoter

T7

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pGBDU-Atg17(F317D, I325A)

Plasmid

#122275

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to Aspartic acid; Isoleucine 32…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(F317D, I325A)

Plasmid

#122273

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to Aspartic acid; Isoleucine 32…

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCu-GFP-Atg17(416)

Plasmid

#121408

Purpose

Expressing GFP-Atg17

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

CUP1p-GFP-ATG17 ORF-CYC1 terminator

Expression

Yeast

Promoter

CUP1 promoter

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(L313D I314K)

Plasmid

#121390

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Leucine 313 to Aspartic acid; Isoleucine 314 to L…

Promoter

ADH1 promoter

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCu-Atg17(416) (L105D)

Plasmid

#121405

Purpose

Expressing Atg17

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

CUP1p-ATG17 ORF-CYC1 terminator

Expression

Yeast

Mutation

Leucine 105 to Aspartic acid

Promoter

CUP1 promoter

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCu-Atg17(416) (F317D)

Plasmid

#121406

Purpose

Expressing Atg17

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

CUP1p-ATG17 ORF-CYC1 terminator

Expression

Yeast

Mutation

Phenylalanine 317 to Aspartic acid

Promoter

CUP1 promoter

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCu-Atg17(416) (I325D)

Plasmid

#121407

Purpose

Expressing Atg17

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

CUP1p-ATG17 ORF-CYC1 terminator

Expression

Yeast

Mutation

Isoleucine 325 to Aspartic acid

Promoter

CUP1 promoter

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag RagC (D181N)

Plasmid

#112752

Purpose

express Flag-RagC (D181N mutant)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (D181N) (RRAGC Human)

Tags

Flag

Expression

Mammalian

Mutation

181N

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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LRG-2.1T-sgTAF12(human)#4.5

Plasmid

#105987

Purpose

lentivirally express gRNA targeting human TAF12 HFD with GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

gRNA targeting human TAF12-HFD

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Sesn-C mSesn2 (delAB)

Plasmid

#111799

Purpose

Mammalian Expression of mSesn2

Depositor

Jun Hee Lee

Article

Ro et al FEBS J. 2014 Sep;281(17):3816-27. doi: 1

Insert

mSesn2 (Sesn2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

AB domain truncation

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Sesn-B+C mSesn2 (delA)

Plasmid

#111798

Purpose

Mammalian Expression of mSesn2

Depositor

Jun Hee Lee

Article

Ro et al FEBS J. 2014 Sep;281(17):3816-27. doi: 1

Insert

mSesn2 (Sesn2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

A domain truncation

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Sesn-A mSesn2 (delBC)

Plasmid

#111797

Purpose

Mammalian Expression of mSesn2

Depositor

Jun Hee Lee

Article

Ro et al FEBS J. 2014 Sep;281(17):3816-27. doi: 1

Insert

mSesn2 (Sesn2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

BC domain truncation

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1alpha-HA-TFAP4(E135A/S139A)

Plasmid

#110159

Purpose

Lentiviral vector for expression of human TFAP4 (E135A/S139A) in mammalian cells

Depositor

Daniele Guardavaccaro

Article

D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

Insert

TFAP4 (transcription factor AP-4) (TFAP4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

E135A/S139A

Promoter

CMV

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-CenpB 1-166

Plasmid

#108474

Purpose

expression of vsv-CenpB 1-166

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

CenpB 1-166 AA (CENPB Human)

Tags

VSV

Expression

Mammalian

Mutation

encodes only the DNA-binding domain of CENP-B

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-Mis12-dCEN-Incenp

Plasmid

#108477

Purpose

expression of vsv-Mis12-dCEN-INCENP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12-dCEN-INCENP (INCENP Human)

Tags

VSV

Expression

Mammalian

Mutation

CEN box replaced by Mis12

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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B3GALT5_pCSdest

Plasmid

#53941

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

B3GALT5 (B3GALT5 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
FAM3B_pCSdest

Plasmid

#53933

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

FAM3B (FAM3B Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
ITGB2_pCSdest

Plasmid

#53892

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

ITGB2 (ITGB2 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits