We narrowed to 5,895 results for: SUP

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Lenti-sgZmat3.51.5/Cre

Plasmid

#167855

Purpose

Expresses Cre recombinase and an sgRNA targeting Zmat3

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

gRNA targeting Zmat3 (Zmat3 Mouse)

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Expression

Mammalian

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pUC18 Apln5-HA-FlpO-pA-LoxP-PGK-Neo-pA-LoxP-Apln3 (SO89)

Plasmid

#159222

Purpose

Can be used to target the mouse Apln locus in mouse eggs or ES cells, in order to drive the mosaic expression of the protein HA-NLS-FlpO

Depositor

Rui Benedito

Article

Luo et al Nature. 2021 Jan;589(7842):437-441. doi:

Insert

Apln 5'-FlpO-WPRE-Sv40pA-LoxP-PGK-Neo-pA-LoxP-Apln

Expression

Mammalian

Promoter

Apln

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSECC-sgMARK4-A

Plasmid

#138676

Purpose

Expresses a mouse MARK4-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgMARK4 mouse (Mark4 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSECC-sgMARK4-B

Plasmid

#138677

Purpose

Expresses a mouse MARK4-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgMARK4 mouse (Mark4 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Blast-sgMARK4

Plasmid

#138707

Purpose

Expresses a human MARK4-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgMARK4 human (MARK4 Human)

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Neo-sgCRTC2-1

Plasmid

#138702

Purpose

Expresses a human CRTC2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgCRTC2 human (CRTC2 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgBRSK2

Plasmid

#138687

Purpose

Expresses a human BRSK2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgBRSK2 human (BRSK2 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Blast-sgBRSK1

Plasmid

#138705

Purpose

Expresses a human BRSK1-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgBRSK1 human (BRSK1 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgBRSK1

Plasmid

#138686

Purpose

Expresses a human BRSK1-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgBRSK1 human (BRSK1 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Neo-sgCRTC2-2

Plasmid

#138703

Purpose

Expresses a human CRTC2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgCRTC2 human (CRTC2 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDM304_YFP-IqgC_mRFP-Raf1(RBD)

Plasmid

#128698

Purpose

simultaneous expression of YFP-tagged IqgC and mRFP-tagged active Ras probe (GTPase binding domain from Raf1 kinase) in Dictyostelium discoideum cells

Depositor

Igor Weber

Article

Marinovic et al Proc Natl Acad Sci U S A. 2019 Jan 22;11

Inserts

iqgC (full length)
Raf1 kinase (aa 50-132)

Use

Dictyostelium discoideum expression vector

Tags

YFP and mRFP

Mutation

none

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCMV-Blast mLST8-Flag Mut1-2

Plasmid

#124918

Purpose

For stable expression sgRNA resistant Flag tagging mLST8 Q44E/N46L and W272F/W274F mutant

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MLST8 (MLST8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1 delta69-97

Plasmid

#124924

Purpose

For expression of a.a.69-97 deletion mutant of SIN1-GFP

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Tags

TagGFP2

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)

Plasmid

#62580

Purpose

Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

SPRED1 (SPRED1 Human)

Use

Luciferase

Mutation

miR-21 binding site 1 mutant (TAAGCTA --> TAGA…

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2)

Plasmid

#62581

Purpose

Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was mutated.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

SPRED1 (SPRED1 Human)

Use

Luciferase

Mutation

miR-21 binding site 2 mutant (TAAGCTA --> TAGA…

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
tdEos-EB3-7

Plasmid

#57610

Purpose

Localization: MT End Binding Protein, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

EB3 (MAPRE3 Human)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pET21a-MKP7_K62R_153

Plasmid

#37795

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

Insert

Mitogen-activated protein kinase phosphatase 7 (DUSP16 Human)

Tags

His

Expression

Bacterial

Mutation

changed Lysnie 62 to Arginine and deleted amino a…

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pET21a-MKP7_K52R_153

Plasmid

#37793

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

Insert

Mitogen-activated protein kinase phosphatase 7 (DUSP16 Human)

Tags

His

Expression

Bacterial

Mutation

changed Lysnie 52 to Arginine and deleted amino a…

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pCDH-EF1-FHC-POLQ-DY2230AA

Plasmid

#64878

Purpose

lentiviral expression of human POLQ -DY2230AA mutant (a polymerase domain mutant)

Depositor

Richard Wood

Article

Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

Insert

POLQ (POLQ Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

D2330A,Y2331A, in the DNA polymerase domain (POL)…

Promoter

EF1alpha

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-GAL4-UAS-activemTGFb1-IRES-mCherry-PGK-BFP

Plasmid

#223213

Purpose

Lentiviral vector - mCherry reporter and TGFb1 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

Depositor

Wendell Lim

Article

Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

Insert

TGFb1 active - membrane (TGFB1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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