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pSADuet760

Plasmid

#45613

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

MjaTFIIB mini intein

Tags

GB1 and H6-GB1

Expression

Bacterial

Promoter

T7

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pSABAD250

Plasmid

#45612

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

NpuDnaBC39

Tags

GB1-H6

Expression

Bacterial

Promoter

pBAD

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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MST4

Plasmid

#39172

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

MST4 (STK26 Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pCS2-Flag-Kdrl-5p-FokI-EL

Plasmid

#35684

Depositor

Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

Kdrl-5p-FokI-EL

Use

Mrna production

Tags

FLAG and Flag

Available Since

April 10, 2012

Availability

Academic Institutions and Nonprofits only
pLPP6

Plasmid

#209968

Purpose

Contains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertion

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter Dropout (sfGFP)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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mCherry-C1-ORP5deltaORDdeltaTMD-FKBP

Plasmid

#220074

Purpose

expresses ORP5 with the OSBP-related ligand-binding domain and the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

OSBPL5deltaORDdeltaTMD (OSBPL5 Human)

Expression

Mammalian

Mutation

deltaORDdeltaTMD

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-ChREBPα

Plasmid

#211754

Purpose

Cloning vector for the expression of mouse ChREBPα

Depositor

Russell Goodman

Article

Singh et al Cell Metab. 2024 Jan 2;36(1):144-158.e7.

Insert

MLXIPL (Mlxipl Mouse)

Use

Cloning vector

Available Since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGL2_171

Plasmid

#199103

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pWKS1 ORI plus NGS barcode 17, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_169

Plasmid

#199101

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pIJ101 ORI plus NGS barcode 15, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_173

Plasmid

#199105

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBBR1-UP ORI plus NGS barcode 21, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPPC017.109

Plasmid

#171145

Purpose

Expression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-KmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

J109 scRNA
J1-BBa_J23117-mRFP

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 and J1-BBa_J23117

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMOD3-mClover-nat

Plasmid

#120328

Purpose

Bacterial mClover promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mClover

Expression

Bacterial

Mutation

D103N (see comments below)

Promoter

(None)

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pSLBPres

Plasmid

#82454

Purpose

Encodes synthesized SLBP that is resistant to SMARTpool siRNA

Depositor

Bharat Ramratnam

Article

Li et al J Clin Invest. 2016 Jul 25. pii: 82360.

Insert

SLBP (SLBP Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only
pT7ddx19gRNA

Plasmid

#47932

Purpose

in vitro transcription of ddx19 gRNA

Depositor

Wenbiao Chen, Susan Wente

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

ddx19 gRNA (ddx19 Zebrafish)

Use

CRISPR

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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GMDS

Plasmid

#39152

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

GMDS (GMDS Human)

Tags

His Tev

Expression

Bacterial

Mutation

codon-optimized

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pPK720

Plasmid

#38147

Depositor

Patricia Kuwabara

Article

Soloviev et al Dev Biol. 2011 Mar 15;351(2):242-53. Epu

Insert

dpy-7p::ptc-3::gfp (ptc-3 )

Tags

EGFP

Expression

Worm

Promoter

dpy-7

Available Since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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6969 pNOTAGHsp104(K620T)

Plasmid

#1237

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Hsp104 (K620T) (HSP104 Budding Yeast)

Expression

Bacterial

Mutation

K620T

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
mCherry-C1-ORP5deltaTMD-FKBP

Plasmid

#220073

Purpose

expresses ORP5 with the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

OSBPL5deltaTMD (OSBPL5 Human)

Expression

Mammalian

Mutation

deltaTMD

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-MPRA-MluI-SpeI-EcoRI

Plasmid

#190196

Purpose

Empty vector for inserting MPRA libraries into AAV

Depositor

Hyejung Won

Article

McAfee et al Cell Genom. 2023 Sep 15;3(10):100404. do

Type

Empty backbone

Use

AAV; Massively parallel reporter assay (mpra)

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_ASCC3_GRIK2

Plasmid

#205774

Purpose

Express mEGFP-tagged fusion protein, ASCC3_GRIK2 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ASCC3_GRIK2 (ASCC3, GRIK2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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