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Ecoli cpGFP

Plasmid

#215713

Purpose

Expresses cpGFP in Ecoli

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

cpGFP

Tags

6xHis

Expression

Bacterial

Mutation

circularly permuted GFP

Promoter

T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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smHA multi-frame tag

Plasmid

#128611

Purpose

a spaghetti monster with 10x HA epitopes repeat upstream of the HIV frameshift sequence which is followed by FLAG epitopes in the 0ORF are separated from one another by SunTag epitopes in the -1ORF.

Depositor

Tim Stasevich

Article

Lyon et al Mol Cell. 2019 Jul 11;75(1):172-183.e9.

Insert

smHA MF tag

Expression

Mammalian

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-hygro-EGFP-Stx17

Plasmid

#174652

Purpose

Vector for overexpression of EGFP-tagged Stx17 with IRES for hygromycin selection.

Depositor

Lukas Kapitein

Article

Janssen et al J Cell Sci. 2021 Oct 1;134(19). pii: 271

Insert

EGFP-Stx17 (STX17 Human)

Use

Lentiviral

Tags

EGFP

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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TRE:mCherry-p2a- bReaChES

Plasmid

#163037

Purpose

FLiCRE reporter gene for neuronal expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

mCherry-p2a-bReaChES

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-GFP-CaM-uTEVp

Plasmid

#163028

Purpose

FLiCRE protease for HEK293T expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

GFP-CaM-uTEVp

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pWS2247

Plasmid

#123026

Purpose

Tet promoter (TetO(x7)-pLEU2m)

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pTet [2]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-CaM-uTEVp

Plasmid

#163032

Purpose

FLiCRE protease for neuronal expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

GFP-CaM-uTEVp

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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CRISPR_TRAC

Plasmid

#164993

Purpose

Expression of gRNA targeting TCRalpha constant locus

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against TRAC

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMIAII-Cd4

Plasmid

#194401

Purpose

Retroviral expression of mouse CD4

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

Cd4 (Cd4 Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-HDAC6-FLAG

Plasmid

#30482

Depositor

Tso-Pang Yao

Article

Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

Insert

HDAC6 (HDAC6 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP(C1)-PP1alpha

Plasmid

#44224

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Article

Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

Insert

PP1A (PPP1CA Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only

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CAG-Blimp1

Plasmid

#73995

Purpose

Plasmid expressing mouse Blimp1

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

Blimp1 gene (Prdm1 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE zeo PPP2CA Wt

Plasmid

#10689

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

protein phosphatase 2, catalytic subunit, alpha isozyme (PPP2CA Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-sTomato-NLS-blast

Plasmid

#136259

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

sTomato-NLS

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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CMVdel-dimericTomato-WASp(CRIB)

Plasmid

#191450

Purpose

Visualizing endogenous & active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

WASp

Expression

Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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Venus rGBD

Plasmid

#99015

Purpose

Used as a RhoA-GTP sensor

Depositor

Narasimhan Gautam

Article

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Insert

rGBD (Rtkn Mouse)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP(C1)-PP1gamma

Plasmid

#44225

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Article

Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

Insert

PP-1G (PPP1CC Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pCB7 alpha5

Plasmid

#16041

Depositor

Filippo Giancotti

Article

Giancotti et al Cell. 1990 Mar 9. 60(5):849-59.

Insert

alpha5 (ITGA5 Human)

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pHR_Gal4UAS_Tbet_T2A_GFP_PGK_mCherry

Plasmid

#85431

Purpose

Lentiviral vector for Gal4 inducible Tbet T2A GFP and constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

human Tbet

Use

Lentiviral

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-mNeonGreen-NLS-blast

Plasmid

#136258

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mNeonGreen-NLS

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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