We narrowed to 11,977 results for: pcDNA

1. Yellow Cameleon-Nano140/pcDNA3

   Plasmid

   #51966

   Purpose

   mammalian expression of Yellow Cameleon-Nano140, an ultrasensitive Ca2+ indicator with Kd = 140 nM

   Depositor

   Takeharu Nagai

   Article

   Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

   Insert

   Yellow Cameleon-Nano140
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-mas-KB2123-Nluc

   Plasmid

   #204325

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   KB2123-Nluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA3.1-StrepHA-SUMO2(Q87R)

   Plasmid

   #66868

   Purpose

   Mammalian expression of SUMO2(Q87R) with a strep and an HA tag

   Depositor

   Ying Liu

   Article

   Bursomanno et al J Proteome Res. 2015 Apr 3;14(4):1687-99

   Insert

   SUMO2 (SUMO2 Human)
   

   Use

   Tags

   HA and Strep

   Expression

   Mammalian

   Mutation

   Q87R

   Promoter

   CMV

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-EGFP-ELYS-polyA

   Plasmid

   #59746

   Purpose

   Express EGFP-ELYS in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   EGFP-ELYS
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1/NDUFV2/myc-His

   Plasmid

   #127491

   Purpose

   Expresses myc-tagged NDUFV2 in mammalian cells

   Depositor

   Dieter Wolf, Shashi Jain

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFV2 (NDUFV2 Human)
   

   Use

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-LBR-EGFP-polyA

   Plasmid

   #59748

   Purpose

   Express LBR-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   LBR-EGFP
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 CDK9 (D167N) HA (P#811)

   Plasmid

   #14641

   Depositor

   Matija Peterlin

   Article

   Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

   Insert

   cdk9 D167N (CDK9 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D167N. Kinase negative mutant. Please see deposit…

   Promoter

   Available since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 Golgi-Ras-LOCKR-S

   Plasmid

   #216499

   Purpose

   FRET-based reporter for Ras activity, Golgi localized

   Depositor

   David Baker

   Article

   Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

   Insert

   Golgi-Ras-LOCKR-S
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 Mito-Ras-LOCKR-S

   Plasmid

   #216501

   Purpose

   FRET-based reporter for Ras activity, mitochondria localized

   Depositor

   David Baker

   Article

   Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

   Insert

   Mito-Ras-LOCKR-S
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-emerin-EGFP-polyA

    Plasmid

    #59747

    Purpose

    Express emerin-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    emerin-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-c/o NL4-3 Env delta CT

    Plasmid

    #139285

    Purpose

    HIV-1 NL4-3 glycoprotein lacking cytoplasmic tail

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    codon optimized HIV-1 Env delta cytoplasmic tail
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of cytoplasmic tail after residue 710

    Promoter

    CMV IE

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO-GAlpha12-RLuc8

    Plasmid

    #140985

    Purpose

    Encodes a G alpha subunit (GNA12) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlpha12-RLuc8 (GNA12 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA/golgin-160/myc

    Plasmid

    #180632

    Purpose

    Expression of WT golgin-160

    Depositor

    Carolyn Machamer

    Article

    Hicks et al J Biol Chem. 2002 Sep 27. 277(39):35833-

    Insert

    golgin-160 (GOLGA3 Human)
    

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 CDK7 D155A HA (P#812)

    Plasmid

    #14648

    Depositor

    Matija Peterlin

    Article

    Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

    Insert

    Cdk7 (CDK7 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D155A: dominant negative

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1-mCherry-CyrCR1.miR16

    Plasmid

    #128747

    Purpose

    Expresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-16 site inserted in mCherry 3'UTR.

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Cyrano conserved region (CR1) with miR-16 site replacing miR-7 site (OIP5-AS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-mas-KB-1753-Nluc

    Plasmid

    #158603

    Purpose

    Plasmid expressing KB-1753-Nluc in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 - linker (GGTGGS) - Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pcdna6.2- myc ULK1 k46I

    Plasmid

    #27630

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse myc ULK1 K46I (Myc Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Promoter

    Available since

    Feb. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA5/FRT/TO HIS HSPH1

    Plasmid

    #19534

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 105kDa/110kDa protein 1 (HSPH1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1/Puro-CAG-VSFP-CR

    Plasmid

    #40257

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    VSFP-CR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNAI Neo human PECAM FL

    Plasmid

    #42902

    Depositor

    Steven Albelda

    Insert

    PECAM (PECAM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2013

    Availability

    Academic Institutions and Nonprofits only