We narrowed to 4,548 results for: BRE

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pF CAG luc puro

Plasmid

#67501

Purpose

Constitutive expression of firefly luciferase in mammalian cells

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

Firefly luciferase

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP VAMP7 (1-220)

Plasmid

#42316

Depositor

Thierry Galli

Article

Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

Insert

VAMP7 (1-220) (VAMP7 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Beta3

Plasmid

#140988

Purpose

Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GBeta3 (GNB3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-GAlphasS-RLuc8

Plasmid

#140980

Purpose

Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GAlphasS-RLuc8

Use

Luciferase

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pF CAG luc hygro

Plasmid

#67502

Purpose

Constitutive expression of firefly luciferase in mammalian cells

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

Firefly luciferase

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pEND-351_Cacnes_dCas9_CRISPRi

Plasmid

#225617

Purpose

Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Insert

dCas9

Use

CRISPR and Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Codon optimized for Cutibacterium acnes

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GGamma1-GFP2

Plasmid

#140989

Purpose

Encodes a G gamma subunit (GNGT1) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GGamma1-GFP2 (GNGT1 Human)

Tags

GFP2 and GSAG linker

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Beta2

Plasmid

#166772

Purpose

Encodes a Gbeta subunit as a "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GNB2 (GNB2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Beta4

Plasmid

#166773

Purpose

Encodes a Gbeta subunit as a "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GNB4 (GNB4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-GRET

Plasmid

#158222

Purpose

Control lentiviral plasmid encoding GFP-Nluc BRET reporter from Schaub et al., Cancer Research, 2015.

Depositor

Charles P. Lai

Article

Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

Insert

GFP-Nluc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA Myc DBC1

Plasmid

#35096

Depositor

Osamu Hiraike

Article

Hiraike et al Br J Cancer. 2010 Mar 16;102(6):1061-7.

Insert

deleted in breast cancer 1 (CCAR2 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX VAMP7 (1-188)

Plasmid

#42315

Depositor

Thierry Galli

Article

Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

Insert

VAMP7 Cytoplasmic domain (1-181) (VAMP7 Human)

Tags

GST

Expression

Bacterial

Mutation

Δ181-220

Promoter

Ptac

Available Since

March 12, 2013

Availability

Academic Institutions and Nonprofits only
pET28a(+) NRP1 b1 (273-427)

Plasmid

#162734

Purpose

Bacterial expression of the b1 domain from the human Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

Depositor

Brett Collins

Article

Daly et al Science. 2020 Oct 20. pii: science.abd30

Insert

Human NRP1 b1 domain residues 273-427

Expression

Bacterial

Mutation

Codon optimised

Available Since

April 27, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Gluc-LHCGR

Plasmid

#221781

Purpose

Measurement of glycoprotein-glycoprotein receptor binding using the BRET phenomenon and measurement of membrane expression of the Gluc-LHCGR

Depositor

Adolfo Rivero-Muller

Article

Adamczuk et al Endocrinology. 2024 Apr 29;165(6):bqae05

Insert

LHCGR (LHCGR Human)

Tags

Gaussia Luciferase and HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-HA-CAD S1859A

Plasmid

#46239

Purpose

FLAG-HA-CAD with S1859 phosphorylation site mutated to alanine

Depositor

Brendan Manning

Article

Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

Insert

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Serine 1859 changed to Alanine (S1859A)

Promoter

cmv

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only
pGEX-TEVsite-human Glomulin

Plasmid

#52292

Purpose

bacterial expression of human Glomulin

Depositor

Brenda Schulman

Article

Duda et al Mol Cell. 2012 Aug 10;47(3):371-82. doi:

Insert

Glomulin (GLMN Human)

Tags

GST and TEV

Expression

Bacterial

Promoter

tac

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pET28a(+) NRP1 b1b2 (273-586)

Plasmid

#162733

Purpose

Bacterial expression the b1b2 tandem domains from the rat Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

Depositor

Brett Collins

Article

Daly et al Science. 2020 Oct 20. pii: science.abd30

Insert

Human NRP1 b1b2 domains (residues 273-586)

Tags

6His

Expression

Bacterial

Mutation

Codon optimised

Available Since

March 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-hSyn-Galpha-s ONE-GO

Plasmid

#214371

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-s ONE-GO (GNAS Human)

Use

Lentiviral

Promoter

hSyn

Available Since

Feb. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-SV40-Galpha-s ONE-GO

Plasmid

#204327

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

SV40 Galpha-s ONE-GO (GNAS Human)

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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p-mCherry-C1-Aurora

Plasmid

#98167

Purpose

red-shifted artificial anion conducting channelrhodopsin (aACR). Activation up to 600 nm; off-kinetics 260 ms. Codon optimized for mammalian expression.

Depositor

Peter Hegemann

Article

Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

Insert

Synthetic construct Aurora gene

Tags

mCherry

Expression

Mammalian

Mutation

V59S, E83N, E90Q, E101S, V117R, E123S, P242R, A24…

Promoter

CMV (+enhancer)

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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