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pJK040 (pET-PurQ-A128T)

Plasmid

#73840

Purpose

Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)

Depositor

T. J. Kappock, JoAnne Stubbe

Article

Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

Insert

formylglycinamidine ribonucleotide synthetase, glutaminase subunit

Expression

Bacterial

Mutation

A128T mutant

Promoter

T7

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pJBL-RNAOUTA4

Plasmid

#69458

Purpose

RNA-OUT A4 variant - antisense (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

Depositor

Julius Lucks

Article

Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

Insert

RNA-OUT A4

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119 and J23119 (sigma 70)

Available Since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pJBL-RNAOUTA3

Plasmid

#69457

Purpose

RNA-OUT A3 variant - antisense (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

Depositor

Julius Lucks

Article

Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

Insert

RNA-OUT A3

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119 and J23119 (sigma 70)

Available Since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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M26L DJ-1 pET15b

Plasmid

#60679

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

Insert

M26L DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

M26L

Promoter

T7

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pDS69

Plasmid

#48367

Purpose

FLAG-intergenic region of TUB-HYG (modified from PMID 16269191)

Depositor

George Cross

Article

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

FLAG-intergenic region of TUB-HYG (modified from PMID 16269191)

Use

Cre/Lox; Knockout in t. brucei

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pLG-deltaA

Plasmid

#33151

Depositor

Michael Rosbash

Article

Legrain et al Cell. 1989 May 19;57(4):573-83.

Insert

RP51A-synthetic minimal intron (RPS17A Budding Yeast)

Tags

LacZ

Expression

Yeast

Mutation

The RP51A sequence was modified by digestion with…

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
His-tagged-SF1 (antisense)

Plasmid

#28183

Depositor

Bernard Schimmer

Article

Frigeri et al Mol Endocrinol. 2000 Apr . 14(4):535-44.

Insert

SF1 (Nr5a1 Mouse)

Tags

His

Expression

Mammalian

Mutation

Reverse Orientation

Available Since

May 24, 2011

Availability

Academic Institutions and Nonprofits only
pWZ414-F48

Plasmid

#23072

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 14 to Glutamine, H3 K14…

Available Since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F50

Plasmid

#23074

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 14 to Arginine, H3 K14R…

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F54

Plasmid

#23078

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 9 to Glutamine, H3 K9Q …

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pJB008

Plasmid

#22647

Depositor

Lynn Hudson

Article

Hudson et al Proc Natl Acad Sci U S A. 1989 Oct . 86(

Insert

Human genomic PLP (PLP1 Human)

Use

Phagemid

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pLIC.B4.TM1717

Plasmid

#11532

Depositor

Sung-Hou Kim

Article

Shin et al Proc Natl Acad Sci U S A. 2004 Sep 7. 10

Insert

YjeQ GDP binding protein (TM1717 T. maritima)

Tags

HisTag-MBP, TEV cleavable

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
piHGpd/T22I

Plasmid

#1110

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with threonine 22 changed to isoleucine; re…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/G81S

Plasmid

#1111

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with glycine 81 changed to serine; regulate…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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piHGpd/G313S

Plasmid

#1114

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with glycine 313 changed to serine; regulat…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/E381K

Plasmid

#1115

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with glutamic acid 381 changed to lysine; r…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/A587T

Plasmid

#1116

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with alanine 587 changed to threonine; regu…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/A41V

Plasmid

#1117

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with alanine 41 changed to valine; regulate…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pRS314-prp16-1

Plasmid

#89972

Purpose

prp16-1 allele in TRP plasmid

Depositor

Christine Guthrie

Article

Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

Insert

PRP16 (PRP16 Budding Yeast)

Expression

Yeast

Mutation

prp16-1 allele (Y386D)

Availability

Academic Institutions and Nonprofits only

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pRS314-prp16-101

Plasmid

#89973

Purpose

prp16-101 allele in TRP plasmid

Depositor

Christine Guthrie

Article

Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

Insert

PRP16 (PRP16 Budding Yeast)

Expression

Yeast

Mutation

prp16-101 allele (G373S)

Availability

Academic Institutions and Nonprofits only

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