162,160 results

1. AiP20142 - pAAV-AiE2638m-minBG-iCre(R297T)-BGHpA

   Plasmid

   #230803

   Purpose

   AiE2638m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   The Allen Institute for Brain Science, Bosiljka Tasic

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   iCre(R297T)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBG

   Available since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. kiCAP-AAV-BI30

   Plasmid

   #183749

   Purpose

   AAV-BI30 Rep-Cap plasmid for production of AAV-BI30, a capsid with tropism for CNS endothelial cells.

   Depositor

   Ben Deverman

   Article

   Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

   Insert

   AAV9 modified with 7mer insertion between amino acids 588 and 589 of VP1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   K449R, and NNSTRGG inserted between 588 and 589 o…

   Promoter

   p41

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-Ykt6

   Plasmid

   #209888

   Purpose

   Expresses mCherry tagged Ykt6 in mammalian cells

   Depositor

   Geert van den Bogaart

   Insert

   Ykt6
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-mRFP

   Plasmid

   #13032

   Purpose

   Mammalian expression vector for cloning your gene fused to mRFP

   Depositor

   Doug Golenbock

   Has Service

   Cloning Grade DNA

   Insert

   Monomer Red Fluorescent Protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3xFLAG-EYFP

   Plasmid

   #34582

   Depositor

   Celeste Nelson

   Article

   Lee et al EMBO J. 2011 May 24;30(13):2662-74. doi:

   Type

   Empty backbone

   Use

   Tags

   3xFLAG and EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-CMV-MLH1dn-IRES-puroR

   Plasmid

   #207354

   Purpose

   Lentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   MutL homolog 1 (MLH1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Δ754-756 dominant negative mutant

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

   Plasmid

   #154473

   Purpose

   Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

   Depositor

   Mikko Taipale

   Article

   Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

   Insert

   ZIM3 (ZIM3 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Includes ZIM3 aa 1-100

   Promoter

   SFFV

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEJS1099: Dual-sgRNA.Design 4

   Plasmid

   #159537

   Purpose

   Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

   Depositor

   Erik Sontheimer

   Article

   Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

   Insert

   Nme2Cas9 with two guide RNA cassettes
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15b-AavLEA1

   Plasmid

   #53093

   Purpose

   AavLEA1 expression (in BL21 DE3 E. coli strain)

   Depositor

   Claude Férec

   Article

   Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi:

   Insert

   AavLEA1
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-MBP-super TEV protease

    Plasmid

    #171782

    Purpose

    Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    MBP-sTEV
    

    Use

    Tags

    Arg6 tag and Internal His6 tag

    Expression

    Bacterial

    Mutation

    No internal TEV cleavage site between the MBP and…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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