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We narrowed to 14,145 results for: TIM

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Showing: 8881 - 8920 of 14145 results

  1. pORFE1052

    Plasmid
    #112078
    Purpose
    Overexpression of the AtCAS9 nickase in plant under 2x35S promoter
    Depositor
    Christian Chevalier
    Insert
    AtCAS9 D10A
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Plant codon optimized nCas9 D10A S. pyogenes
    Promoter
    2x35S
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112078-plasmid-map-sequence-id-217613

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  2. pORFE0014

    Plasmid
    #112076
    Purpose
    AtCAS9 nickase D10A module CDS1ns
    Depositor
    Christian Chevalier
    Insert
    AtCAS9 D10A without stop codon
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Available Since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112076-plasmid-map-sequence-id-217621

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  3. FU-H2B-GFP-IRES-Puro

    Plasmid
    #69550
    Purpose
    Lentiviral, selectable fluorescent GFP tagging of nuclei.
    Depositor
    Charles Gersbach
    Insert
    Histone 2B - green fluorescent protein fusion
    Use
    Lentiviral
    Tags
    GFP fusion
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69550-plasmid-map-sequence-id-122943

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  4. pME-dCas9-BFP-KRAB

    Plasmid
    #221502
    Purpose
    CRISPRi machinery fish codon optimized
    Depositor
    Alessandro Ori
    Insert
    dCas9-tagBFP-KRAB
    Use
    Fish expression
    Mutation
    D10A and H839A point mutations
    Available Since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221502-plasmid-map-sequence-id-439413

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  5. CMV mRuby3-Synapsin1a

    Plasmid
    #187896
    Purpose
    For labeling pre-synaptic terminals
    Depositor
    Timothy Ryan
    Insert
    Synapsin1a
    Tags
    mRuby3
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    187896-plasmid-map-sequence-id-394752

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  6. pPB-spCas9-pNeo

    Plasmid
    #214120
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    spCas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available Since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    214120-plasmid-map-sequence-id-423617

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  7. pH6-TEV-mCherry

    Plasmid
    #132430
    Purpose
    Expresses His6-tagged mCherry in E. coli using T7 promoter
    Depositor
    Mark Arbing
    Insert
    mCherry
    Tags
    N-terminal His6 tag with TEV protease site
    Expression
    Bacterial
    Mutation
    Codon optimized
    Promoter
    T7
    Available Since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    132430-plasmid-map-sequence-id-258769

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  8. pET15-EF1p_mFAP2b

    Plasmid
    #159469
    Purpose
    Encodes for EF1p_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF1p_mFAP2b
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Available Since
    Dec. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    159469-plasmid-map-sequence-id-319725

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  9. pET15-EF4n_mFAP2b

    Plasmid
    #159464
    Purpose
    Encodes for EF4n_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF4n_mFAP2b
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Available Since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159464-plasmid-map-sequence-id-316655

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  10. pET15-EF1p3_mFAP2b

    Plasmid
    #159470
    Purpose
    Encodes for EF1p3_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF1p3_mFAP2b
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Available Since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159470-plasmid-map-sequence-id-316659

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  11. pET15-mFAP10

    Plasmid
    #159463
    Purpose
    Encodes for mFAP10 with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    mFAP10
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Available Since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159463-plasmid-map-sequence-id-316643

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  12. pMB22.SV40.Zeo

    Plasmid
    #135692
    Purpose
    Lentivirus delivery for stable expression of AsCas12a-VPR, Zeocin selection
    Depositor
    Florian Karreth
    Insert
    mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator
    Use
    Lentiviral
    Available Since
    Feb. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    135692-plasmid-map-sequence-id-322223

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  13. FgH1tUTG_Bfl-1.1

    Plasmid
    #85536
    Purpose
    Inducible expression of guide RNA (Bfl-1.1) with fluorescent GFP reporter
    Depositor
    Marco Herold
    Insert
    Bfl1.1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    H1t
    Available Since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85536-plasmid-map-sequence-id-169887

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  14. FgH1tUTG_Bfl-1.2

    Plasmid
    #85537
    Purpose
    Inducible expression of guide RNA (Bfl-1.2) with fluorescent GFP reporter
    Depositor
    Marco Herold
    Insert
    Bfl1.2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    H1t
    Available Since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85537-plasmid-map-sequence-id-169893

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  15. pPB-spCas9-pZeo

    Plasmid
    #214121
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    spCas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available Since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    214121-plasmid-map-sequence-id-423619

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  16. pET15-EF2n3_mFAP2b

    Plasmid
    #159466
    Purpose
    Encodes for EF2n3_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF2n3_mFAP2b
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Available Since
    Dec. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    159466-plasmid-map-sequence-id-319724

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  17. pET15-EF1n2_mFAP2b

    Plasmid
    #159471
    Purpose
    Encodes for EF1n2_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF1n2_mFAP2b
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Available Since
    Dec. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    159471-plasmid-map-sequence-id-319723

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  18. pET15-EF1n3_mFAP2b

    Plasmid
    #159472
    Purpose
    Encodes for EF1n3_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF1n3_mFAP2b
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Available Since
    Dec. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    159472-plasmid-map-sequence-id-319720

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  19. pET15-EF1n_mFAP2b

    Plasmid
    #159473
    Purpose
    Encodes for EF1n_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF1n_mFAP2b
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Available Since
    Dec. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159473-plasmid-map-sequence-id-316660

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  20. pET15-EF2n_mFAP2b

    Plasmid
    #159468
    Purpose
    Encodes for EF2n_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF2n_mFAP2b
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Available Since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159468-plasmid-map-sequence-id-316657

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  21. pET15-EF2n2_mFAP2b

    Plasmid
    #159467
    Purpose
    Encodes for EF2n2_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF2n2_mFAP2b
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Available Since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159467-plasmid-map-sequence-id-316656

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  22. R777-E237 Hs.RASAL3

    Plasmid
    #70521
    Purpose
    Gateway ORF clone of codon optimized human RASAL3 [NM_022904.1] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASAL3 (RASAL3 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70521-plasmid-map-sequence-id-125970

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  23. pPB-CAG-PlatTET-wpA-pPuroTK

    Plasmid
    #214125
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    dCas9-TET
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available Since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    214125-plasmid-map-sequence-id-424237

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  24. pcDNA4_ChR2opt_mKATE

    Plasmid
    #67958
    Purpose
    optical depolarization of mammalian cells
    Depositor
    Andreas Offenhäusser
    Insert
    Channelrhodopsin 2 opt
    Tags
    mKatushka
    Expression
    Mammalian
    Mutation
    synthetically produced for mammalian optimized co…
    Promoter
    CMV -TetON
    Available Since
    Sept. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    67958-plasmid-map-sequence-id-118415

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  25. pORFE0005

    Plasmid
    #112073
    Purpose
    AtDeadCAS9 D10A H840A without stop codon module CDS1ns
    Depositor
    Christian Chevalier
    Insert
    AtDeadCAS9 D10A H840A without stop codon
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Available Since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112073-plasmid-map-sequence-id-217642

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  26. hSyn1-FLEX-hChR2-tdtomato

    Plasmid
    #41015
    Depositor
    Karel Svoboda
    Insert
    Channelrhodopsin-2
    Use
    Adenoviral
    Tags
    tdtomato
    Expression
    Mammalian
    Mutation
    codon-optimized (human)
    Promoter
    synapsin
    Available Since
    Nov. 6, 2012
    Availability
    Academic Institutions and Nonprofits only

  27. pKP1469

    Plasmid
    #160815
    Purpose
    Expression of ExbB protein; the exbB gene replaces tonB gene in BamHI site of pKP325; includes pBAD promoter and optimized RBS from pBAD24-derived pKP660 (ExbB, ExbD)
    Depositor
    Kathleen Postle
    Insert
    exbB
    Expression
    Bacterial
    Available Since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    160815-plasmid-map-sequence-id-316117

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  28. pET15b-ACP (from S. aureus)

    Plasmid
    #63686
    Purpose
    Inducible expression of Acyl Carrier Protein (ACP) from S. aureus
    Depositor
    Michael Johnson
    Insert
    ACP
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized
    Promoter
    T7
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  29. ATSR

    Plasmid
    #50052
    Purpose
    expresses t.gondii VWA+TSR1 domains in mammalian cells
    Depositor
    Timothy Springer
    Insert
    MIC2-ATSR
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    S158A
    Promoter
    Chick beta-actin promoter
    Available Since
    Feb. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  30. proATSR

    Plasmid
    #50053
    Purpose
    Expresses T.gondii prodomain+VWA+TSR1 domains in mammalian cells
    Depositor
    Timothy Springer
    Insert
    MIC2-proATSR
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    S158A
    Promoter
    chick beta-actin promoter
    Available Since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  31. CMV-mt4xi-GCaMP6f

    Plasmid
    #192075
    Purpose
    Fluorescent reporter for mitochondrial calcium
    Depositor
    Timothy Ryan
    Insert
    4 repeats of improved COX8 signal peptide plus GCaMP6f
    Expression
    Mammalian
    Available Since
    Dec. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    192075-plasmid-map-sequence-id-378919

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  32. pLV-mScarlet-I

    Plasmid
    #159172
    Purpose
    To express a bright monomeric red FP for labelling and detection of mammalian cells
    Depositor
    Pantelis Tsoulfas
    Insert
    mScarlet-I
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Mutation
    Codons were optimized to Human codon usage
    Promoter
    CMV
    Available Since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159172-plasmid-map-sequence-id-311154

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  33. pCR2.1-InC

    Plasmid
    #83959
    Purpose
    Contains a synthetic insert to serve as internal control DNA template for a Salmonella Real-Time PCR assay.
    Depositor
    Kai-Shun Chen
    Insert
    Internal Control sequence
    Expression
    Bacterial
    Available Since
    Nov. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    83959-plasmid-map-sequence-id-160468

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  34. R777-E139 Hs.NF1

    Plasmid
    #70423
    Purpose
    Gateway ORF clone of codon optimized human NF1 [NM_000267.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    NF1 (NF1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70423-plasmid-map-sequence-id-125872

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  35. pBabepuro-PDGFB

    Plasmid
    #189611
    Purpose
    Retroviral expression vector for PDGFB. stimulates fibroblastic proliferation (Tchenio T, unpublished, 2008)
    Depositor
    Thierry Tchenio
    Insert
    PDGFB (PDGFB Human)
    Use
    Retroviral
    Promoter
    SV40 early promoter
    Available Since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    189611-plasmid-map-sequence-id-381075

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  36. pICSL4723_ERF922

    Plasmid
    #126883
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126883-plasmid-map-sequence-id-250052

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  37. pHH0103 SMURF2 WW domain #3

    Plasmid
    #104226
    Purpose
    Bacterial expression of WW domain #3 from SMURF2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    SMURF2 WW domain #3 (SMURF2 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104226-plasmid-map-sequence-id-204295

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  38. pHH0103 SMURF2 WW domain #2

    Plasmid
    #104225
    Purpose
    Bacterial expression of WW domain #2 from SMURF2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    SMURF2 WW domain #2 (SMURF2 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    104225-plasmid-map-sequence-id-204192

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  39. pHH0103 SMURF2 WW domain #1

    Plasmid
    #104224
    Purpose
    Bacterial expression of WW domain #1 from SMURF2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    SMURF2 WW domain #1 (SMURF2 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    104224-plasmid-map-sequence-id-201513

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  40. pHH0103 WBP4 WW domain #2

    Plasmid
    #104253
    Purpose
    Bacterial expression of WW domain #2 from WBP4
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    WBP4 WW domain #2 (WBP4 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104253-plasmid-map-sequence-id-204383

    Enlarge

Showing: 8881 - 8920 of 14145 results