We narrowed to 5,495 results for: pcDNA3.1

Showing: 4441 - 4460 of 5495 results

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pcDNA-p160(1-200)

Plasmid

#43

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Tags

myc

Expression

Mammalian

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pTE3987

Plasmid

#137716

Purpose

Expresses dLbCas12a-RVRR-BE in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

Insert

dLbCas12a-RVRR-BE

Tags

6xHis, APOBEC-1, SV40 NLS, and UGI

Expression

Mammalian

Mutation

G532A, K538V, Y542R, K595R

Promoter

CMV

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-p160(400-580)

Plasmid

#45

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Tags

myc

Expression

Mammalian

Mutation

expresses only aa400-580

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA-p160(200-400)

Plasmid

#44

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Tags

myc

Expression

Mammalian

Mutation

Expresses only aa200-400

Available Since

July 21, 2005

Availability

Academic Institutions and Nonprofits only
hMEF2A K403R

Plasmid

#118355

Purpose

This plasmid expresses human MEF2A K403R mutant. This construct cannot undergo sumoylation on K403.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hMEF2A (MEF2A Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K403R

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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Myc-delta

Plasmid

#118037

Purpose

GABAA receptor expression (delta subunit) Myc-tag near N-terminus

Depositor

Angel de Blas

Article

Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14.

Insert

GABRD (Gabrd Rat)

Tags

cMyc epitope EQKLISEEDL inserted between fourth a…

Expression

Mammalian

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 RL23Cm6

Plasmid

#109367

Purpose

Human rod opsin chimera with intracellular loops 2-3 and C-terminus from mGluR6

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Rod opsin-mGluR6 chimera (RHO, GRM6 Human)

Tags

1D4

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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CoV2-N-P13L

Plasmid

#177946

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P13L). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (P13L) (N )

Expression

Mammalian

Mutation

P13L

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-N-P80R

Plasmid

#177947

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (P80R) (N )

Expression

Mammalian

Mutation

P80R

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-N-S235F

Plasmid

#177956

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S235F). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (S235F) (N )

Expression

Mammalian

Mutation

S235F

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-FLJ23445

Plasmid

#15429

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

FLJ23445 (Thoc7 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
CNC3 (CASANOVA-C3)

Plasmid

#137191

Purpose

Expresses CNC3 (CASANOVA-C3), a blue light dependent NmeCas9 inhibitory protein

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2021 Mar 18;49(5):e29

Insert

CNC3 (CASANOVA-C3)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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1029-AIDx-CP

Plasmid

#135341

Purpose

Expression of circular permutant nSpyCas9 at amino acid position 1029 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

CP 1029 nSpyCas9-hAIDx

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-TBX1wt-IDR

Plasmid

#145254

Purpose

Expresses fusion of GAL4 DNA-binding domain and TBX1wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

TBX1wt-IDR (TBX1 Human)

Expression

Mammalian

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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Myc-PCDHGC5

Plasmid

#119935

Purpose

Protocadherin gammaC5 expression (Myc-tag is at the linker between EC1 and EC2 extracellular cadherin repeats)

Depositor

Angel de Blas

Article

Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

Insert

PCDHGC5 (Pcdhgc5 Rat)

Tags

9E10 cMyc epitope (EQKLISEEDL) was inserted betwe…

Expression

Mammalian

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA/Bmal I317D

Plasmid

#47347

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

I317D

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
Opto-GPR25

Plasmid

#106011

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR25; Opto-GPR25; B1)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR25 (GPR25 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA/Bmal L115E

Plasmid

#47345

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

L115E

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
PCDNA3-N3FLAG-MYB-(1-327)

Plasmid

#105612

Purpose

transient express MYB N terminal fragment with 3*FLAG tag at N terminal in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

N terminal fragment of MYB (mouse), amino acids 1-327 (Myb Mouse)

Tags

3*FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-N3FLAG-MYB-TAD(251-327)

Plasmid

#105611

Purpose

transient express MYB TAD fragment with 3*FLAG tag at N terminal in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAD fragment of MYB (mouse), amino acids 251-327 (Myb Mouse)

Tags

3*FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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