We narrowed to 9,202 results for: sacs

1. YIplac204-TDH3p-LDH-CYC1t

   Plasmid

   #216121

   Purpose

   Yeast integrative vector for constitutive expression of LDH at the TRP1 locus

   Depositor

   Yvonne Nygård

   Article

   Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

   Insert

   Lactate dehydrogenase (LDH Bovine, Bos taurus)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH3 promoter

   Available since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAM59

   Plasmid

   #227655

   Purpose

   pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, Δ78–81)

   Depositor

   Andreas Martin

   Article

   Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-

   Inserts

   Rpn11 (RPN11 Budding Yeast)
   Rpn8 (RPN8 Budding Yeast)
   

   Use

   Tags

   H6-PreScission and PreScission-Strep

   Expression

   Bacterial

   Mutation

   1-179 and amino acids 2-239, Δ78–81

   Promoter

   T7

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAM57

   Plasmid

   #227654

   Purpose

   pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, V80A)

   Depositor

   Andreas Martin

   Article

   Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-

   Inserts

   Rpn11 (RPN11 Budding Yeast)
   Rpn8 (RPN8 Budding Yeast)
   

   Use

   Tags

   H6-PreScission and PreScission-Strep

   Expression

   Bacterial

   Mutation

   1-179 and amino acids 2-239, V80A

   Promoter

   T7

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAM54

   Plasmid

   #227653

   Purpose

   pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, L132A)

   Depositor

   Andreas Martin

   Article

   Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-

   Inserts

   Rpn11 (RPN11 Budding Yeast)
   Rpn8 (RPN8 Budding Yeast)
   

   Use

   Tags

   H6-PreScission and PreScission-Strep

   Expression

   Bacterial

   Mutation

   1-179 and amino acids 2-239, L132A

   Promoter

   T7

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAM71

   Plasmid

   #227621

   Purpose

   pETDuet-1 H6-precission-Rpn11(24-160-GGSGSG-192-221) , Rpn8(1-179)

   Depositor

   Andreas Martin

   Article

   Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d

   Inserts

   Rpn11 (RPN11 Budding Yeast)
   rpn8 (RPN8 Budding Yeast)
   

   Use

   Tags

   His-Prescission

   Expression

   Bacterial

   Mutation

   1-179 and amino acids 24-160-GGSGSG-192-221

   Promoter

   T7

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a- mCherry-FIVAR-HIS-ybbR

   Plasmid

   #169138

   Purpose

   E. coli expression of mCherry domain containing ybbR tag and FIVAR fingerprint domain. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   mCherry
   

   Use

   Tags

   6x Histag, FIVAR tag, and ybbr tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST17-ScChd1[R126A/R130A]

   Plasmid

   #221197

   Purpose

   ScChd1-aa118-1274 chromatin remodeler with R126A and R130A mutations

   Depositor

   Gregory Bowman

   Article

   Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

   Insert

   ScChd1 (aa118-1274)
   

   Use

   Tags

   6xHis-tag (Precission protease cleavable)

   Expression

   Bacterial

   Mutation

   R126A, R130A

   Promoter

   T7

   Available since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST17-ScChd1[∆ChEx]

   Plasmid

   #221199

   Purpose

   ScChd1-aa142-1274 chromatin remodeler with ChEx domain deleted

   Depositor

   Gregory Bowman

   Article

   Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

   Insert

   ScChd1 (aa142-1274)
   

   Use

   Tags

   6xHis-tag (Precission protease cleavable)

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-117 and 1275-1468

   Promoter

   Available since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pWzyE-His

   Plasmid

   #196669

   Purpose

   Expression of C-terminally His10-tagged WzyE from Pectobacterium atrosepticum in E.coli

   Depositor

   James Naismith

   Article

   Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

   Insert

   WzyE
   

   Use

   Tags

   TEV cleavable His10

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ypq2-2GFP-URA

    Plasmid

    #207010

    Purpose

    Integration of 2xGFP tag at YPQ2 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    YPQ2
    

    Use

    Tags

    2xGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Vph1-2GFP-URA

    Plasmid

    #207009

    Purpose

    Integration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    VPH1
    

    Use

    Tags

    2xGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKL2-138

    Plasmid

    #219413

    Purpose

    Estradiol-inducible expression of 42-10 Zinc finger activator

    Depositor

    Ahmad Khalil

    Article

    Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

    Insert

    {pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-4x)-PDZLigWT-tSSA1}
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Four Arg to Ala mutations in zinc finger backbone

    Promoter

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pToxEXPr6

    Plasmid

    #218290

    Purpose

    Introduction of a cyanamide-inducible I-SceI expression cassette and a TetR-TUP1 expression cassette, use together with pJQR3

    Depositor

    Claudia Vickers

    Article

    McDonnell et al bioRxiv 2024.03.26.586389

    Insert

    hph>tCYC1-ISceI.site-Repeat2-pDDI2>ISceI>tSynth3-pHAC1>TetR>TUP1>tABF1-HO(182,1979)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJQR3

    Plasmid

    #218289

    Purpose

    Introduction of a Zif268-hER-VP16 expression cassette and a LmrA-Mig1C expression cassette, use together with pToxEXPR6

    Depositor

    Claudia Vickers

    Article

    McDonnell et al bioRxiv 2024.03.26.586389

    Insert

    HO(-253,-1)-pADH1>LmrA>MIG1(aa123-504)>tMIG1-tYGL036W

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pITEdv1

    Plasmid

    #218283

    Purpose

    Use together with with pJE13HD7 and pJE13HD7 derivatives to integrate heterologous genes at ho locus

    Depositor

    Claudia Vickers

    Article

    Evans et al bioRxiv 2024.01.30.578080

    Insert

    Hph>tAgTEF1-ISceI-Repeat1-HO(1828, 1979)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pITEu2

    Plasmid

    #218282

    Purpose

    Use together with pToxEXP/pToxAmp plasmids to insert heterologous genes at ho locus

    Depositor

    Claudia Vickers

    Article

    Evans et al bioRxiv 2024.01.30.578080

    Insert

    HO(-253, -1)-Repeat2-ISceI-pAgTEF1>Hph(partial)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJE13HD7

    Plasmid

    #218281

    Purpose

    Use together with pITEdv1, or pToxEXP1/pToxAmp plasmids to insert heterologous gene(s) at ho locus.

    Depositor

    Claudia Vickers

    Article

    Evans et al bioRxiv 2024.01.30.578080

    Insert

    HO(-253, -1)-Repeat1-ISceI-pAgTEF1>Hph(partial)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRH2941

    Plasmid

    #210143

    Purpose

    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::ARO7-GFP::tADH1

    Depositor

    Randy Hampton

    Article

    Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

    Insert

    YIp ADE2-LEU2 pTDH3::ARO7-GFP::tADH1 (ARO7 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBDP

    Plasmid

    #217827

    Purpose

    Stuffer plasmid with golden gate compatible bidirectional promoter

    Depositor

    Timothy Whitehead

    Article

    Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

    Insert

    Gal1/Gal10 Bidirectional Promoter
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-h.Rhno1 Plk1mut7A-C-PAMmut-Myc-DDK-P2A-Puro

    Plasmid

    #211616

    Purpose

    Rhno1 Plk1mut7A

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    hRHNO1 (RHNO1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S51A, T52A, S141A, S162A, S163A, S164A, S178A

    Promoter

    CMV

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFastBac1-His6-E3(1)-CUbo-TwinStrep

    Plasmid

    #212712

    Purpose

    Expression of His6-E3(1)-CUbo-TwinStrep in insect cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(1)
    

    Use

    Tags

    6xHis and CUbo-TwinStrep

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. P1K-3HA-Atg8∆R-MET15

    Plasmid

    #207034

    Purpose

    Expression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Use

    Tags

    3xHA

    Expression

    Yeast

    Mutation

    ∆R

    Promoter

    pATG8

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. YIp128-TET-NUbo-E3(63)-VSV

    Plasmid

    #212752

    Purpose

    Doxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(63)
    

    Use

    Integrative vector

    Tags

    NUbo and VSV

    Expression

    Yeast

    Mutation

    Promoter

    Tet

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. YIp211-TET-NUbo-E3(63)-VSV

    Plasmid

    #212753

    Purpose

    Doxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(63)
    

    Use

    Integrative vector

    Tags

    NUbo and VSV

    Expression

    Yeast

    Mutation

    Promoter

    Tet

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. YIp211-TET-NUbo-E3(48)-VSV

    Plasmid

    #212744

    Purpose

    Doxycycline-inducible expression of NUbo-E3(48)-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(48)
    

    Use

    Integrative vector

    Tags

    NUbo and VSV

    Expression

    Yeast

    Mutation

    Promoter

    Tet

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. YIp128-TET-NUbo-E3(48)-VSV

    Plasmid

    #212743

    Purpose

    Doxycycline-inducible expression of NUbo-E3(48)-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(48)
    

    Use

    Integrative vector

    Tags

    NUbo and VSV

    Expression

    Yeast

    Mutation

    Promoter

    Tet

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pYSD140

    Plasmid

    #212927

    Purpose

    Encodes the mRuby2 fluorescent protein (Part3) without any signal peptide assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    mRuby2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA5-FRT-TO-NUbo-E3(63)-FLAG

    Plasmid

    #212808

    Purpose

    Constitutive or doxycycline-inducible expression of NUbo-E3(63)-FLAG in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(63)
    

    Use

    Tags

    FLAG and NUbo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA5-FRT-TO-NUbo-FLAG

    Plasmid

    #212797

    Purpose

    Constitutive/Doxycycline-inducible expression of NUbo-FLAG in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    NUbo
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRH3105

    Plasmid

    #210164

    Purpose

    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-P194Q-GFP::tADH1

    Depositor

    Randy Hampton

    Article

    Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

    Insert

    YIp ADE2-LEU2 pTDH3::lys1-P194Q-GFP::tADH1 (LYS1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    P194Q

    Promoter

    Available since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge