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AAV-hSyn-SpdCas9-W3SL

Plasmid

#210708

Purpose

This Plasmid express hSyn promoter driven SpdCas9

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

S. Pyogenes dCas9

Use

AAV and CRISPR

Tags

FLAG

Expression

Mammalian

Mutation

D10A/H840A

Promoter

hSyn

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS1-HA-KDEL.SV40(polyA)

Plasmid

#156164

Purpose

Expresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus and KDEL (ER retention) sequence

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

THBS1 with addition of ER-retention sequence KDEL

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-hSncg-EGFP

Plasmid

#153198

Purpose

Human gamma-synuclein (hSncg) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a DIO-ChRmine-oScarlet-Kv2.1-WPRE

Plasmid

#183525

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

ChRmine-oScarlet-Kv2.1

Use

AAV

Promoter

Ef1a

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-hM3D(Gq)-mCherry

Plasmid

#223652

Purpose

Expresses hM3D(Gq) in a flippase dependent manner

Depositor

Oscar Marin

Article

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

hM3D(Gq)-mCherry

Use

AAV

Tags

mCherry

Promoter

hSyn

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6/TO-shEsr1-CMV-TetR-eGFP

Plasmid

#233298

Purpose

DOX-inducible U6 driven shRNA against mouse Esr1

Depositor

Dayu Lin

Article

Golden et al bioRxiv [Preprint]. 2024 Sep 25:2023.12.

Insert

Esr1 shRNA (Esr1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-VEGF(Brolucizumab) PAGER(Gq)

Plasmid

#230014

Purpose

Expresses anti-VEGF PAGER(Gq) in mammalian cell

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-Brolucizumab-TEVcs-ALFA-hM1D

Use

AAV

Expression

Mammalian

Mutation

W442A on hM1D

Promoter

CMV

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-GFP(LaG16) PAGER(Gq)

Plasmid

#230006

Purpose

Expresses anti-GFP PAGER(Gq) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaG16-TEVcs-ALFA-hM1D

Use

AAV

Expression

Mammalian

Mutation

W442A on hM1D

Promoter

CMV

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-IvfChr-citrine-KV2.1

Plasmid

#221615

Purpose

A recombinant AAV2 plasmid encoding vfChrimson-citrine with trafficking enhancement and soma targeting with KV2.1 motif.

Depositor

John Lin

Article

Mermet-Joret et al eLife 2023 12:RP90327

Insert

vfChrimson-citrine with membrane trafficking enhancement and soma targeting

Use

AAV

Mutation

vfChrimson-citrine with membrane trafficking enha…

Promoter

Human Synapsin

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CW3SL-Myr-mCherry-Dr-TrkA

Plasmid

#127962

Purpose

Myr mCherry DrTrkA expression under CAMK promoter

Depositor

Vladislav Verkhusha

Article

Leopold et al Nat Commun. 2019 Mar 8;10(1):1129. doi:

Insert

mCherry DrTrkA

Use

AAV

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-Gluc-RMA-IRES-EGFP

Plasmid

#189630

Purpose

Expresses Gluc-RMA and EGFP in the presence of Cre recombinase. Double-floxed Gluc-RMA and EGFP for monitoring Cre-expressing neuronal populations.

Depositor

Jerzy Szablowski

Article

Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Inserts

Gaussia luciferase fused to Fc
IRES-EGFP

Use

AAV, Cre/Lox, and Luciferase

Tags

Gluc and IgG1 Fc

Expression

Mammalian

Promoter

IRES and hSyn

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP

Plasmid

#176279

Purpose

Viral vector for co-expression of non-functional Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

Depositor

Martin Riccomagno

Article

Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

Kir2.1MUT-P2A-EGFP (Kcnj2 Synthetic, Mouse)

Use

AAV and Cre/Lox

Tags

Myc

Expression

Mammalian

Mutation

GYG to AAA (aa144-146)

Promoter

human Synapsin I

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAVLINK-EF1alpha-HA-5'-MYO5C

Plasmid

#244348

Purpose

5' AAVLINK plasmid for MYO5C expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

MYO5C (MYO5C Human)

Use

AAV

Tags

HA

Promoter

EF1alpha

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-spCas9

Plasmid

#231404

Purpose

Expresses spCas9 from hSyn promoter

Depositor

Arjen Boender, Larry Young

Article

Schimmer et al Nat Commun. 2026 Feb 20;17(1):1932. doi:

Insert

hSYN promoter (RIC8B Human)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

hSYN

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-eGFP-P2A-LgBiT-NLS

Plasmid

#187834

Purpose

Expression of eGFP-P2A-LgBiT-NLS from human synapsin promoter via adeno-associated virus for neuronal expression.

Depositor

William McEwan

Article

Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

Insert

eGFP-P2A-LgBiT-NLS

Use

AAV

Promoter

hSyn

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-hBFP-2A-RVG-WPRE

Plasmid

#120268

Purpose

Permits retrograde transsynaptic spread by driving Cre-dependent co-expression of the RbG plus a nuclear reporter

Depositor

Edward Callaway

Article

Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

Insert

EF1α-DIO-H2B-tagBFP-Flagx3-T2Am-cB19G

Use

AAV

Expression

Mammalian

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pRC_RR_VP2/3

Plasmid

#65725

Purpose

wt VP2/VP3 only to participate in capsid assembly; no ITR's

Depositor

Junghae Suh

Article

Judd et al Mol Ther Nucleic Acids. 2012 Nov 13;1:e5

Insert

empty

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-rsChRmine-eYFP-WPRE

Plasmid

#183531

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

rsChRmine-eYFP

Use

AAV

Mutation

I146M/G174S

Promoter

CaMKIIa

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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HT115_pAAV_hSyn-DiO-SomQuasAr6b_EGFP-P2A-somCheRiff_HA

Plasmid

#178827

Purpose

Cre-on bicistronic expression of QuasAr6b-based Optopatch under an neuronal promoter

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

SomQuasAr6a_EGFP-P2A-somCheRiff_HA

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

EGFP, HA

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS3-TeTLc-P2A-mCherry

Plasmid

#178708

Purpose

AAV vector for Flp-dependent transgene expression of TeTLc-P2A-mCherry in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

TeTLc-P2A-mCherry

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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