We narrowed to 9,265 results for: yeast expression

1. pNTI733 pRPR1(TetO)-RPC31-sgRNA

   Plasmid

   #164913

   Purpose

   For yeast genomic integration of sgRNA against RPC31

   Depositor

   Nicholas Ingolia

   Article

   Muller et al Science. 2020 Dec 11;370(6522). pii: 370

   Insert

   pRPR1(TetO)-RPC31-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   RPR1

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNTI732 pRPR1(TetO)-HTS1-sgRNA

   Plasmid

   #164912

   Purpose

   For yeast genomic integration of sgRNA against HTS1

   Depositor

   Nicholas Ingolia

   Article

   Muller et al Science. 2020 Dec 11;370(6522). pii: 370

   Insert

   pRPR1(TetO)-HTS1-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   RPR1

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB2336

   Plasmid

   #67541

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB2229

   Plasmid

   #67537

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked CaLYS5 marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJRL2 GAL1(TATA-A1T6)pr VYFP (EB#1573)

   Plasmid

   #14849

   Depositor

   Erin O'Shea

   Article

   Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

   Insert

   GAL1 promoter TATA-A1T6 (GAL1 Budding Yeast)
   

   Use

   Tags

   VYFP

   Expression

   Yeast

   Mutation

   TATA-A1T6. TATA box mutated from TATATAAG to AATA…

   Promoter

   Available Since

   May 25, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pJRL2 GAL1(TATA-A1T6)pr ECFP (EB#1574)

   Plasmid

   #14850

   Depositor

   Erin O'Shea

   Article

   Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

   Insert

   GAL1 promoter TATA-A1T6 (GAL1 Budding Yeast)
   

   Use

   Tags

   ECFP

   Expression

   Yeast

   Mutation

   TATA-A1T6. TATA box mutated from TATATAAG to AATA…

   Promoter

   Available Since

   May 25, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pJRL2 GAL1(TATA-C2)pr VYFP (EB#1577)

   Plasmid

   #14851

   Depositor

   Erin O'Shea

   Article

   Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

   Insert

   GAL1 promoter TATA-C2 (GAL1 Budding Yeast)
   

   Use

   Tags

   VYFP

   Expression

   Yeast

   Mutation

   TATA-C2. TATA box mutated from TATATAAG to TCTATA…

   Promoter

   Available Since

   May 25, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pJRL2 GAL1(TATA-C2)pr ECFP (EB#1578)

   Plasmid

   #14852

   Depositor

   Erin O'Shea

   Article

   Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

   Insert

   GAL1 promoter TATA-C2 (GAL1 Budding Yeast)
   

   Use

   Tags

   ECFP

   Expression

   Yeast

   Mutation

   TATA-C2. TATA box mutated from TATATAAG to TCTATA…

   Promoter

   Available Since

   May 25, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pV1093

   Plasmid

   #111428

   Purpose

   CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1

   Depositor

   Gerald Fink

   Article

   Vyas et al Sci Adv. 2015;1(3):e1500248.

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. PAP-delta10-cHIS

    Plasmid

    #21716

    Depositor

    Andrew Bohm

    Article

    Balbo et al Biochemistry. 2005 May 31. 44(21):7777-8

    Insert

    polyA polymerase (PAP1 Budding Yeast)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    30 amino acids off the c terminus

    Promoter

    Available Since

    Aug. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pMetYC-Dest

    Plasmid

    #105081

    Purpose

    Yeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.

    Depositor

    Christopher Grefen

    Article

    Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

    Type

    Empty backbone

    Use

    Tags

    Cub-PLV

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPRyl_XPR2

    Plasmid

    #84609

    Purpose

    Introduces DSB at XPR2 locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. YIplac211-KAR2-msGFP2-HDEL

    Plasmid

    #160468

    Purpose

    Yeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to msGFP2 with an ER retrieval signal

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    KAR2-msGFP2-HDEL
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. bRA66

    Plasmid

    #100952

    Purpose

    GAL1 driven Cas9. gRNA can be cloned into BplI sites.

    Depositor

    James Haber

    Article

    Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRISPRyl_AXP

    Plasmid

    #84608

    Purpose

    Introduces DSB at AXP locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR221-Sen1

    Plasmid

    #99994

    Purpose

    Shuttle yeast Sen1 into Gateway destination vector

    Depositor

    Susan Baserga

    Article

    Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

    Insert

    Sen1 (SEN1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCRISPRyl_D17

    Plasmid

    #84611

    Purpose

    Introduces DSB at D17 locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCRISPRyl_A08

    Plasmid

    #84610

    Purpose

    Introduces DSB at A08 locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCRISPRyl_MFE1

    Plasmid

    #84612

    Purpose

    Introduces DSB at MFE1 locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCCL-GKL1RC-Short-LEUg1mKateg2GKL2ORF10UCS

    Plasmid

    #52345

    Purpose

    Integration cassette to create p1-Short-Leu-mKate with p2 ORF10 UCS>mKate

    Depositor

    Chang Liu

    Article

    Ravikumar et al Nat Chem Biol. 2014 Mar;10(3):175-7. doi

    Insert

    mKate2
    

    Use

    P1 integration vector

    Tags

    Expression

    Bacterial

    Mutation

    changed V2H

    Promoter

    pGKL2 ORF10 UCS

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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