We narrowed to 18,370 results for: Tec

1. pTS2365-Tier1-PhCMV-TetR

   Plasmid

   #169566

   Purpose

   Tier-1 vector encoding PhCMV-driven tetracycline dependent regulator protein TetR (PhCMV-TetR-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven tetracycline repressor protein class B from transposon Tn10
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHBintT

   Plasmid

   #48134

   Purpose

   Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vector

   Depositor

   Dieter Jahn

   Article

   Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. CP1249-ABEmax

   Plasmid

   #119809

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   ecTadA-ecTadA*(7.10)-nSpCas9 (CP1249)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBiex-1 Myosin VI HMM_SNAP

   Plasmid

   #82716

   Purpose

   Expresses Myosin VI HMM with a C-terminal SNAP tag.

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Hariadi et al Nat Nanotechnol. 2015 Aug;10(8):696-700.

   Insert

   Myosin VI
   

   Use

   Tags

   6xHis Tag, FLAG purification tag, and SNAP tag

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   T7 Promoter

   Available since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. GWF297

   Plasmid

   #133612

   Purpose

   TET3G-2xMS2(wt+f6), NLS-MCP-ANR-ANR-P2a-BFP

   Depositor

   Dustin Maly

   Article

   Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

   Insert

   TET3G-2xMS2(wt+f6), MCP-ANR-ANR, BFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. CL7-LbCas12aD156R

   Plasmid

   #164659

   Purpose

   Bacterial expression of mutated LbCas12aD156R using CL7/Im7

   Depositor

   Piyush Jain

   Article

   Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

   Insert

   LbCas12a-D156R
   

   Use

   Tags

   Thioredoxin, CL7, SUMO

   Expression

   Bacterial

   Mutation

   changed Aspartic Acid 156 to Arginine

   Promoter

   T7

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGGF_RT_FAST_RFP

   Plasmid

   #136979

   Purpose

   Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence

   Depositor

   Klaus Theres

   Article

   Rossmann et al Plant J. 2019 Oct 30. doi: 10.1111/tpj.1

   Insert

   seed coat marker RFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   BsaI site domestication in pOLE1:OLE1-RFP 688 G &…

   Promoter

   Available since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-TO-Sp dCas9-mVenusN

   Plasmid

   #191396

   Purpose

   SpdCas9 fused to N-terminal part of mVenus

   Depositor

   Albert Jeltsch

   Article

   Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

   Insert

   N-terminal part of split mVenus
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAC-4CL1

   Plasmid

   #35947

   Depositor

   Claudia Schmidt-dannert

   Article

   Watts et al BMC Biotechnol. 2006 Mar 21;6:22.

   Insert

   4-coumaroyl CoA ligase 1 (4CL1 Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLac-mod

   Available since

   Sept. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBabe hygro NFS1

    Plasmid

    #102977

    Purpose

    Expression of NFS1

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    NFS1 (NFS1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations to eliminate shNFS1_1 binding si…

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. YSBE 508 : pNEO_GFP

    Plasmid

    #92081

    Purpose

    Plasmid for tagging proteins in Cryptococcus neoformans; GFP tag, Neo selection

    Depositor

    Yong-Sun Bahn

    Article

    So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

    Insert

    GFP
    

    Use

    tagging proteins in cryptococcus neoforman

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSPlit-OC-hDib1

    Plasmid

    #58789

    Purpose

    Express hDib1-LucC fusion protein in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    hDib1 (TXNL4A Human)
    

    Use

    Tags

    Flag and Luciferase (394-550aa)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

13. AAV-CMV-LOXP-stop-LOXP-mG-CaMP3.0

    Plasmid

    #50022

    Purpose

    expresses Membrane tagged G-CaMP3 in cells expressing Cre

    Depositor

    David Anderson

    Article

    Vrontou et al Nature. 2013 Jan 31;493(7434):669-73. do

    Insert

    G-CaMP3
    

    Use

    AAV

    Tags

    MARCKS sequence (MGCCFSKT)

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 Flag-MBD1-14-mVenusC

    Plasmid

    #191393

    Purpose

    MBD domain of MBD1 fused to C-terminal part of split mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    pcDNA3 Flag-MBD1-14-mVenusC
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Marathon-RT tetramutant for Split-PE (pEH101)

    Plasmid

    #190109

    Purpose

    Expresses Marathon-RT tetramutant (D14R-D74R-N116K-N197R)

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    Marathon-RT
    

    Use

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    D14R, D74R, N116K, N197R

    Promoter

    CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTK312

    Plasmid

    #59569

    Purpose

    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP XbaI attB1 Kozak EGFP ochre attB2 AscI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)-HaloTag N58-(GGS)X6-FRB

    Plasmid

    #171427

    Purpose

    Expression of HaloTag N58-(GGS)X6-FRB in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    HaloTag N58-(GGS)X6-FRB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOCC154

    Plasmid

    #118902

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    

    Use

    Tags

    TwinStrep, cleavable with 3C protease

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ-NF-L3-NF-L1-dMb2Cas12a

    Plasmid

    #177686

    Purpose

    Gateway entry clone (attL1 & attR5) for a single chain FokI-dMb2Cas12a genome editing

    Depositor

    Yiping Qi

    Article

    Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

    Insert

    2xFokI-dMb2Cas12a
    

    Use

    CRISPR; Gateway compatible 2xfoki-dmb2cas12a entr…

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU3-gRNA

    Plasmid

    #53063

    Purpose

    Transcription of guide RNA (gRNA) in rice cells

    Depositor

    Caixia Gao

    Article

    Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

    Insert

    OsU3 promoter + gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    Expression

    Mutation

    Promoter

    rice U3

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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