We narrowed to 14,348 results for: TIM

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SPHK1 gRNA (BRDN0001148403)

Plasmid

#76815

Purpose

3rd generation lentiviral gRNA plasmid targeting human SPHK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SPHK1 (SPHK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLVU-coHPDL WT

Plasmid

#174128

Purpose

Expresses wild type codon-optimized HPDL in mammalian cells

Depositor

Michael Pacold

Article

Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

Insert

4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Codon optimized

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-ORAI1-EC-HA

Plasmid

#174335

Purpose

encodes hORAI1 with HA-tag in its extracellular loop 2 and an N-terminal GFP fusion (GFP-ORAI1-EC-HA).

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human Orai1 (ORAI1 Human)

Tags

GFP in the N-terminus and HA tag in the extracell…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pJR107

Plasmid

#187245

Purpose

Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter

Use

Lentiviral

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-GAL4-iLight-VP16

Plasmid

#170274

Purpose

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16

Use

AAV

Mutation

See Comments

Promoter

CaMKII

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCA13-pHR-SFFV-HaloTag-FBL

Plasmid

#199444

Purpose

Expression of HaloTag-FBL in mammalian cells

Depositor

Baohui Chen

Article

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

FBL (FBL Human)

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

SFFV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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SiriusGFP

Plasmid

#123201

Purpose

We have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.

Depositor

Joseph Santos-Sacchi

Article

Zhong et al J Neurosci Methods. 2019 Feb 1;313:68-76

Insert

SiriusGFP

Use

Mouse Targeting

Tags

N/A

Expression

Bacterial and Mammalian

Mutation

eGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ1415

Plasmid

#84828

Purpose

Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-1 introns

Depositor

Andrew Fire

Article

Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

Insert

C. elegans codon optimized GFP

Expression

Bacterial and Worm

Promoter

Peft-3

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJR106

Plasmid

#187244

Purpose

Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter

Use

Lentiviral

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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PIK3C2B gRNA (BRDN0001149054)

Plasmid

#76712

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3C2B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3C2B (PIK3C2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Cox7c-pHLuorin-sems

Plasmid

#107766

Purpose

labeling of mitochondrial cytochrome c oxidase, pH measurements

Depositor

Karin Busch

Article

Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

Insert

Subunit Cox7c fused to pHluorin (COX7C Human)

Tags

pHluorin

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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PIK3C2B gRNA (BRDN0001149112)

Plasmid

#76713

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3C2B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3C2B (PIK3C2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINT2

Plasmid

#127512

Purpose

Plasmid encodes H. sapiens codon optimized Integrase 2.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 2 coding sequence codon optimized for H. sapiens expression.

Expression

Mammalian

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINT9

Plasmid

#127516

Purpose

Plasmid encodes H. sapiens codon optimized Integrase 9.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 9 coding sequence codon optimized for H. sapiens expression.

Expression

Mammalian

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINT13

Plasmid

#127517

Purpose

Plasmid encodes H. sapiens codon optimized Integrase 13.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 13 coding sequence codon optimized for H. sapiens expression.

Expression

Mammalian

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pYFP-ErbB2

Plasmid

#66948

Purpose

Mammalian expression of rat ErbB2 tagged with YFP

Depositor

Mads Lerdrup, Bo van_Deurs

Article

Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

Insert

ErbB2 (Erbb2 Rat)

Tags

6xHis, V5 Tag, and YFP

Expression

Mammalian

Mutation

(See depositor comment below on mutations A24T an…

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pQCX-CD28 WT

Plasmid

#223640

Purpose

Retroviral expression of mouse CD28 in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (Cd28 Mouse)

Use

Retroviral; mammalian expression

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-Syn-DIO-TRPV1-P2A-mCherry

Plasmid

#200831

Purpose

In the presence of Cre, drives neuron-specific expression of TRPV1 with fluorescence reporter mCherry

Depositor

Hong Chen

Article

Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

Insert

TRPV1-P2A-mCherry (Trpv1 Rat)

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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p59sheLL

Plasmid

#40628

Depositor

Simon Beddows

Article

Draper et al Vaccine. 2011 Nov 3;29(47):8585-90. Epub

Inserts

HPV59 L1
HPV59 L2

Expression

Mammalian

Mutation

Codon optimized for mammalian expression

Promoter

CMV and Polio IRES

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only

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p420-3

Plasmid

#71257

Purpose

luciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in front of hGM-CSF -55 to +28 minimal promoter

Depositor

Peter Cockerill

Article

Johnson et al Mol Cell Biol. 2004 Sep;24(18):7914-30.

Insert

3 copies of the GM420 NFAT-AP-1 site (CSF2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

GM-CSF minimal

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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