We narrowed to 9,244 results for: ef1;
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Plasmid#129012PurposeControl for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2 reporterDepositorInsert5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pTDH3-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
ExpressionYeastAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRPL28 SpHIS5 (pNTI663)
Plasmid#115433PurposeExpresses wild-type RPL28 from HIS3 yeast plasmidDepositorInsertsExpressionYeastMutationMfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPL28(Q38P) SpHIS5 (pNTI664)
Plasmid#115434PurposeExpresses Q38P RPL28 from HIS3 yeast plasmidDepositorInsertsExpressionYeastMutationQ38P; MfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPL28(H39L) SpHIS5 (pNTI665)
Plasmid#115435PurposeExpresses H39L RPL28 from HIS3 yeast plasmidDepositorInsertsExpressionYeastMutationH39L; MfeI site in intronPromoterashbya gospii TEF1 and endogenousAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF HA-TSAd-CNS
Plasmid#84558PurposeMammalian expression of TSAd-CNSDepositorAvailable SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1478
Plasmid#82424PurposePlasmid for expression of GST-tagged Nematostella vectensis GW182 in mammalian cellsDepositorInsertnvGW182
TagsGSTExpressionMammalianPromoterEF1AAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEBB-3XMyc-TRAF2 S327A
Plasmid#44108DepositorAvailable SinceMay 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-640a-1
Plasmid#42092DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseExpressionMammalianMutationcontains the 820-842 nt segment of ZEB1 mRNA (NM_…Available SinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-640s-3
Plasmid#42091DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseExpressionMammalianMutationcontains the 4565-4587 nt segment of ZEB1 mRNA (N…Available SinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-302s-1
Plasmid#42087DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseExpressionMammalianMutationcontains the 4146-4168 nt segment of ZEB1 mRNA (N…Available SinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-215a-1
Plasmid#42085DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseExpressionMammalianMutationcontains the 1259-1281 nt segment of ZEB1 mRNA (N…Available SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-640s-2
Plasmid#42090DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseExpressionMammalianMutationcontains the 1956-1981 nt segment of ZEB1 mRNA (N…Available SinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-640s-1
Plasmid#42089DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseExpressionMammalianMutationcontains the 1419-1441 nt segment of ZEB1 mRNA (N…Available SinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-302a-1
Plasmid#42088DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseExpressionMammalianMutationcontains the 4055-4078 nt segment of ZEB1 mRNA (N…Available SinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-640a-2
Plasmid#42093DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseExpressionMammalianMutationcontains the 1635-1657 nt segment of ZEB1 mRNA (N…Available SinceFeb. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-215a-2
Plasmid#42086DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseExpressionMammalianMutationcontains the 2444-2466 nt segment of ZEB1 mRNA (N…Available SinceFeb. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pDIV301
Plasmid#204969PurposeAMA1 plasmid with Aspergillus optimized Mad7 and ble (bleomycin) resistance markerDepositorInsertsMad7
ble (bleomycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2
Plasmid#75112Purposelenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6 and EF1AAvailable SinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
CROP-seq-opti
Plasmid#106280PurposeA version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.DepositorInsertEF1a-Puro-WPRE-hU6-gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only