Addgene

We narrowed to 39,265 results for: lat

Showing: 4481 - 4500 of 39265 results

Results per page:

Sort results by:

CMV-BRAF-REG-Halo

Plasmid

#194771

Purpose

Mammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag.

Depositor

Deborah Morrison

Article

Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e

Insert

BRAF regulatory (REG) domain (AA 1-435) (BRAF Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV51

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-mFgf21pro2-luc

Plasmid

#101798

Purpose

Reporter for mouse Fgf21 promoter activity

Depositor

Seiichi Oyadomari

Article

Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

Insert

Fibroblast growth factor 21 promoter (Fgf21 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus2-Y705F/S727A-STAT3

Plasmid

#123177

Purpose

Expresses Y705F/S727A STAT3 double mutant fused to the 158-238 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)

Use

Tags

Venus2 (aa 158-238) for bimolecular fluorescence …

Expression

Mammalian

Mutation

Double Y705F and S727A substitutions

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPaint-mNeon-BlastR [M1G]

Plasmid

#171805

Purpose

Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and blasticidin resistance cassette [p.M1G]

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mNeonGreen-T2A-BlastR

Use

Gene tagging

Tags

Expression

Mutation

BlastR: Changed Methionin 1 to Glycine.

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-mFgf21pro3-luc

Plasmid

#101799

Purpose

Reporter for mouse Fgf21 promoter activity

Depositor

Seiichi Oyadomari

Article

Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

Insert

Fibroblast growth factor 21 promoter (Fgf21 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST EGFP-flag-CAD

Plasmid

#188118

Purpose

Expresses N-terminal EGFP-tagged/C-terminal flag-tagged human CAD in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

EGFP tag and Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-2xFLAG-2xSTREP-CCND1(P287A)-IRES-mCherry

Plasmid

#172637

Purpose

Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287A) and mCherry in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Pro287Ala

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-Tau-cYFP

Plasmid

#92205

Purpose

Retroviral overexpression vector for α-Syn/Tau bimolecular fluorescence complementation (BiFC)

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

MAPT (MAPT Human)

Use

Retroviral

Tags

YFP(156-239)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only
pTT3-ecdICAM1-ST3-His

Plasmid

#210666

Purpose

Expression of the extracellular domain of human ICAM1 fused to Spytag003 + Histag in HEK293 (or similar)

Depositor

Omer Dushek

Article

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

Extracellular domain of human ICAM-1 fused to Spytag003 and Histag (ICAM1 Human, Synthetic)

Use

Tags

Histag and Spytag003

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-2xFLAG-2xSTREP-CDK6

Plasmid

#172614

Purpose

Expresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDK6 (CDK6 Human)

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-2xFLAG-2xSTREP-CCND2-IRES-mCherry

Plasmid

#172627

Purpose

Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D2 and mCherry in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND2 (CCND2 Human)

Use

Lentiviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus1-Y705F/S727A-STAT3

Plasmid

#123176

Purpose

Expresses Y705F/S727A STAT3 double mutant fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Use

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

Double Y705F and S727A substitutions

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus1-S727A-STAT3

Plasmid

#123174

Purpose

Expresses S727A STAT3 fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Use

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

S727A substitution

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC1012 - pAAV EF1a DIO Nuc-eYFP

Plasmid

#75082

Purpose

An AAV packaging vector that expresses Cre-dependent nuclear-localized eYFP under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

Insert

Nuc-eYFP

Use

AAV and Cre/Lox

Tags

NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FLAG-2xSTREP-CCND3(T283A)-Puro

Plasmid

#172618

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(T283A) and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Thr283Ala

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Tet-O-FUW-GLI1-P2A-mCherry

Plasmid

#203858

Purpose

Tet-inducible lentiviral plasmid expressing GLI1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

GLI1 (GLI1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-Tau-nYFP

Plasmid

#92204

Purpose

Retroviral overexpression vector for Tau bimolecular fluorescence complementation (BiFC)

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

MAPT (MAPT Human)

Use

Retroviral

Tags

YFP(1-155)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FLAG-2xSTREP-CCND3-Puro

Plasmid

#172619

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDN-D2irTNG4kwh

Plasmid

#44722

Depositor

Gabor Balazsi

Article

Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

Inserts

pCMV-D2i promoter
htetR::NLS::EGFP

Use

Synthetic Biology; Expression regulator/reporter;…

Tags

Rabbit β-globin intron II and WPRE

Expression

Mammalian

Mutation

Initiator motif (Inr) displaced relative to pCMV-…

Promoter

pCMV-D2i

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus1-Y705F-STAT3

Plasmid

#123172

Purpose

Expresses Y705F STAT3 fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Use

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

Y705F substitution

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge