We narrowed to 4,634 results for: HRE

1. pLenti6.3-OGDH_wobble

   Plasmid

   #110938

   Purpose

   OGDH ORF containing silent wobble mutations insensitive to corresponding shRNAs

   Depositor

   William Hahn

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

   Insert

   OGDH wobble cDNA (OGDH Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   15 silent wobble mutations corresponding to three…

   Promoter

   SV40

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. p5E-gfap

   Plasmid

   #75024

   Purpose

   Can be used to drive expression in zebrafish astrocytes.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   glial fibrillary acidic protein promoter (gfap Zebrafish)
   

   Use

   5' entry vector for multisite gateway three …

   Tags

   Expression

   Mutation

   Promoter

   gfap

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV2-CAG-3xNLS-AausFP1

   Plasmid

   #191096

   Purpose

   To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods

   Depositor

   Pantelis Tsoulfas, Murray Blackmore

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   3xNLS-AausFP1
   

   Use

   AAV and Synthetic Biology

   Tags

   Three repeats of the nuclear localizzation seque…

   Expression

   Mammalian

   Mutation

   Optimized to Human codon usage

   Promoter

   CMV enhancer and CAG

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3/hArf6(T27N)-HA

   Plasmid

   #79426

   Purpose

   Expresses C-terminally HA-tagged Arf6(T27N) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

   Insert

   Arf6 (ARF6 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed Threonine 27 to Asparagine

   Promoter

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. RCAS-Flag-BRAFV600E

   Plasmid

   #126615

   Purpose

   Retroviral expression of Flag tagged human oncogenic BRAFV600E

   Depositor

   Yi Li

   Article

   Bu et al Cancer Res. 2019 Jan 1;79(1):61-71. doi:

   Insert

   BRAF (BRAF Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   V600E

   Promoter

   Available Since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3xflag-APEX2-LRRK2

   Plasmid

   #164622

   Purpose

   Expresses LRRK2 tagged with APEX2 and 3xFLAG

   Depositor

   Mark Cookson

   Article

   Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   3xFLAG and APEX2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-EGFP MASTL WT (siRNA resistant)

   Plasmid

   #191011

   Purpose

   Expresses EGFP-tagged MASTL WT with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

   Depositor

   Johanna Ivaska

   Article

   Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   Microtubule-associated serine/threonine kinase like (MASTL Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pInducer20 ALPK1 T237M

   Plasmid

   #231586

   Purpose

   ALPK1 gene mutated in position T237M (Pathogenic mutation in ROSAH syndrome) under control of a doxycycline-inducible promoter

   Depositor

   Thomas Henry

   Article

   Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

   Insert

   ALPK1 (ALPK1 Human)
   

   Use

   Lentiviral

   Tags

   3X flag

   Expression

   Mammalian

   Mutation

   changed threonine 237 to Methionine

   Promoter

   doxycycline

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-hSyn-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

   Plasmid

   #177345

   Purpose

   AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from Synapsin promoter

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   dead hSaCas9
   

   Use

   AAV and CRISPR

   Tags

   3x GCN4 peptide, 3xHA, and NLS

   Expression

   Mammalian

   Mutation

   D10A, N580A

   Promoter

   Synapsin

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTH733-2µ-RLuc/maxCFLuc

    Plasmid

    #40608

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. EF1a_MYC Thr58Ala_P2A_Hygro_Barcode

    Plasmid

    #120513

    Purpose

    Barcoded lentiviral vector to express MYC Thr58Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC Thr58Ala (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Point mutation changing Threonine to Alanine at a…

    Promoter

    EF1a

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDN-T2dMFot

    Plasmid

    #44558

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al PLoS Comput Biol. 2012;8(4):e1002480. do

    Inserts

    PTETREG promoter
    rtetR-M2::FFF
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Compared to rtTA, rtTA-M2 has S12G, E19G, A56P, D…

    Promoter

    PTETREG

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-LAP-KNL1-M3

    Plasmid

    #115896

    Purpose

    Used for expression of siRNA-resistant KNL1-M3 (modified codons 258 and 259) from the FRT site of FlpIn cells upon addition of doxycyclin.

    Depositor

    Geert Kops

    Article

    Vleugel et al J Cell Biol. 2013 Dec 23;203(6):943-55.

    Insert

    KNL1-M3 (KNL1 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Fragment of KNL1 containing three KNL1 repeat seq…

    Promoter

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. BE3-NLS-NLS-P2A-BLAST

    Plasmid

    #135348

    Purpose

    Expression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    BE3-2XNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL4.23 A82V/T230A/D637G 2014 EBOV + Mucin-Like Domain

    Plasmid

    #86020

    Purpose

    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

    Depositor

    Jeremy Luban, Pardis Sabeti

    Article

    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

    Insert

    Makona Ebola Virus Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed alanine 82 to valine, threonine 230 to al…

    Promoter

    CMV IE

    Available Since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL4.23 T230A 2014 EBOV Delta-Mucin-Like-Domain

    Plasmid

    #86023

    Purpose

    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

    Depositor

    Jeremy Luban, Pardis Sabeti

    Article

    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

    Insert

    Makona Ebola Virus Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lacks mucin-like domain: Changed threonine 230 to…

    Promoter

    CMV IE

    Available Since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL4.23 A82V/T230A 2014 EBOV Delta-Mucin-Like-Domain

    Plasmid

    #86025

    Purpose

    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

    Depositor

    Jeremy Luban, Pardis Sabeti

    Article

    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

    Insert

    Makona Ebola Virus Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lacks mucin-like domain; Changed alanine 82 to va…

    Promoter

    CMV IE

    Available Since

    July 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-T923G

    Plasmid

    #233063

    Purpose

    Expresses cleavage-deficient mouse ADGRL3-T923G (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.

    Depositor

    Jonathan Javitch

    Article

    Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

    Insert

    ADGRL3 (Adgrl3 Mouse)
    

    Use

    Tags

    ALFA and HiBiT

    Expression

    Mammalian

    Mutation

    Threonine 923 to Glycine

    Promoter

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBiFC-SS-VN155(I152L)-IRE1-dLD

    Plasmid

    #217752

    Purpose

    Expresses the VN fragment of BiFC-IRE1-dLD

    Depositor

    Christina Chan

    Article

    Cho et al Cell Rep. 2019 Apr 2;27(1):307-320.e5. d

    Insert

    Serine/threonine-protein kinase/endoribonuclease IRE1 (ERN1 Human)
    

    Use

    Tags

    Venus (1-154, I152L)

    Expression

    Mammalian

    Mutation

    deleted amino acids 29-408

    Promoter

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC131: pAAV.CMV-CasRx-VEGFA sgRNA array

    Plasmid

    #203444

    Purpose

    Plasmid expressing active RfxCas13d with an array of three VEGFA mRNA targeting gRNAs

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Inserts

    U6-VEGFA sgRNA array
    RfxCas13d
    

    Use

    AAV

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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