We narrowed to 6,300 results for: nls

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pCMV-ABE7.10-P2A-GFP

Plasmid

#162509

Purpose

Express ABE7.10-P2A-GFP by CMV promoter in mammalian cells

Depositor

Yongsub Kim

Article

Shin et al Nucleic Acids Res. 2021 Feb 26;49(4):239

Insert

ABE7.10-P2A-GFP

Use

CRISPR

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pY027

Plasmid

#84742

Purpose

Expresses huLbCpf1 and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huLbCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pcs2+spCas9 ABE-Umax-nanos

Plasmid

#222142

Purpose

Adenine base editor with modified TadA variant for germline A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9 with nanos UTR

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Promoter

SP6

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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dpYPQ230-D156R-ABE5

Plasmid

#195371

Purpose

dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x8

Depositor

Yiping Qi

Article

Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

Insert

ecTadA8e-(GGGGS)x8-dLbCas12a-D156R

Use

CRISPR; Gateway compatible ectada8e-(ggggs)x8-dlb…

Tags

5' and 3' NLS

Expression

Plant

Promoter

ZmUBI1

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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(564) pAAV Alb-AAT KRAB-SadCas9 U6-mPcsk9

Plasmid

#163025

Purpose

Expression of CRISPRi with gRNA against mouse Pcsk9

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9 (Pcsk9 S. aureus)

Use

AAV

Tags

KRAB, SV40 pA, and myc NLS

Expression

Mammalian

Promoter

Alb-AAT

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ233 (dLbCpf1-SRDX)

Plasmid

#86211

Purpose

Deactivated LbCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid

Depositor

Yiping Qi

Article

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Insert

LbCpf1

Use

CRISPR

Tags

5' and 3' NLS and SRDX repressor

Expression

Plant

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-NG-PE2-BSD

Plasmid

#176933

Purpose

Expresses PE2-NG variant in mammalian cells

Depositor

Hyongbum Kim

Article

Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

Insert

PE2-NG variant

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pPB1163

Plasmid

#210032

Purpose

Contains Cas3 gRNA cloning site, inducible Cas11-P2A-Cas6-T2A-Cas3, rtTA-T2A-blast

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas11-P2A-Cas6-T2A-Cas3
rtta-T2A-blast

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF-1α and TRE3G

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3D-BE3

Plasmid

#113413

Purpose

Expresses hA3D-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3D-BE3 (APOBEC3D Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-OC-IRES-BSD

Plasmid

#53118

Purpose

To create stable cell clones with high-level expression of Cas9 and OCT1

Depositor

Wensheng Wei

Article

Zhou et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Inserts

OCT1 (POU2F1 Human)
Cas9

Use

Lentiviral

Tags

IRES-BSD, NLS, and P2A

Expression

Mammalian

Mutation

human codon-optimized

Promoter

CMV

Available Since

May 27, 2014

Availability

Academic Institutions and Nonprofits only
L2_lacZgRNA-Cas9-CsA

Plasmid

#136138

Purpose

Plasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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G1333 DddAtox-N–SaKKH-Cas9(D10A)

Plasmid

#157837

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

G1333 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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dpYPQ230-D156R-ABE8

Plasmid

#195569

Purpose

dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GSAGSAAGSGEF)x3

Depositor

Yiping Qi

Article

Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

Insert

ecTadA8e-(GSAGSAAGSGEF)x3-dLbCas12a-D156R

Use

CRISPR; Gateway compatible ectada8e-(gsagsaagsgef…

Tags

5' and 3' NLS

Expression

Plant

Promoter

ZmUBI1

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pUDC175

Plasmid

#103019

Purpose

S. cerevisae centromic plasmid harboring Fncpf1 under control of TEF1 promoter

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

Fncpf1

Tags

3HA and NLS

Expression

Yeast

Mutation

human codon optimized

Promoter

TEF1

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-deSpCas9

Plasmid

#92114

Purpose

Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive eSpCas9 with FLAG tag

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

D10A, H840A, K848A, K1003A, R1060A

Promoter

Cbh

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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AA332

Plasmid

#216053

Purpose

Fragmid fragment: (C' terminus) 3x NLS sequences

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

SV40NLS_v1.10; SV40NLS_v1.11; SV40NLS_v1.12

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pROF417

Plasmid

#155335

Purpose

Constitutive expression LFY-VP16-2A-RLuc in plants

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

PCaMV35S-LFY-VP16-NLS-2A-RLuc-Tnos

Use

Luciferase and Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV NucleoBAS

Plasmid

#62861

Purpose

Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

Depositor

Stan van de Graaf

Article

van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

Insert

NucleoBAS

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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LLP786_SpyCatcher-EZH2-FL-CO

Plasmid

#211779

Purpose

SpyTag counterpart binding domain, SpyCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

aGCN4-KRAB

Tags

3xTy1

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect KRAB e…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCRI006-pYFAC-riboB-PgpdA-dSpCas9-VPR-TtrpC

Plasmid

#140199

Purpose

Episomal expression of dSpCas9-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Insert

dSpCas9-VPR

Use

CRISPR and Synthetic Biology; Expression in asper…

Tags

VPR (VP64-p65-Rta), NLS

Promoter

PgpdA

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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