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We narrowed to 6,501 results for: nls

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Showing: 4501 - 4520 of 6501 results

  2. SpCas9-HF1-plus

    Plasmid
    #126768
    Purpose
    Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126768-plasmid-map-sequence-id-410966

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  3. lenti-UCOE-dCas9-BFP-Zim3-KRAB

    Plasmid
    #196716
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-TagBFP-KRAB(ZIM3)
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196716-plasmid-map-sequence-id-388040

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  4. pEMS1375

    Plasmid
    #29176
    Purpose
    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple88 (GFAP Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-NLS
    Available since
    Nov. 14, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    29176-plasmid-map-sequence-id-13591

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  5. P3-dCas9-Tet1-GFP-Puro

    Plasmid
    #190728
    Purpose
    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)
    Depositor
    Maja Jagodic
    Publication
    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-tet1
    Use
    CRISPR
    Tags
    3XFLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Changed Pro434 codon from CCG to CCC and Gly468 f…
    Promoter
    pCAG
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    190728-plasmid-map-sequence-id-381333

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  7. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

    Plasmid
    #71828
    Purpose
    Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BACH2-sgRNA8 (BACH2 Human, Synthetic, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    U6
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71828-plasmid-map-sequence-id-131023

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  8. pmiCas9

    Plasmid
    #162758
    Purpose
    express miCas9
    Depositor
    Eugene Chen
    Publication
    Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    miCas9
    Use
    CRISPR
    Tags
    FLAG and NLS
    Expression
    Mammalian
    Mutation
    Contains Brex27 sequence range is 4958-5065
    Promoter
    CMV
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    162758-plasmid-map-sequence-id-329432

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  10. pYPQ266E

    Plasmid
    #161521
    Purpose
    Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 PmCDA1 mediated C-T base editing
    Depositor
    Yiping Qi
    Publication
    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41
    Insert
    zSpRYCas9(D10A)-PmCDA1-UGI
    Use
    CRISPR; Gateway compatible zsprycas9(d10a)-pmcda1…
    Tags
    3X FLAG, NLS
    Expression
    Plant
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    161521-plasmid-map-sequence-id-325638

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  16. PX405 Neisseria meningitidis Cas9

    Plasmid
    #68702
    Purpose
    Neisseria meningitidis Cas9
    Depositor
    Feng Zhang
    Insert
    NmCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pYPQ230-RRV

    Plasmid
    #188543
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editing
    Depositor
    Yiping Qi
    Publication
    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:
    Insert
    LbCas12a-RRV
    Use
    CRISPR; Gateway compatible lbcas12a-rrv entry clo…
    Tags
    NLS
    Expression
    Plant
    Mutation
    G146R, D156R, R182V
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    188543-plasmid-map-sequence-id-369473

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  20. pEMS1172

    Plasmid
    #29301
    Purpose
    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple151 (OLIG1 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-NLS
    Available since
    Jan. 6, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    29301-plasmid-map-sequence-id-13716

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Showing: 4501 - 4520 of 6501 results