We narrowed to 20,388 results for: ACE;
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Plasmid#227297PurposeExpresses SpCas9 and a sgRNA targeting the N-terminus of RAB7A for knock-in.DepositorInsertsgRNA Targeting N-terminus of RAB7A (RAB7A Human)
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Zeo-H2BC11
Plasmid#207759PurposeDonor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorInsertH2BC11 Homology Arms flanking a moxGFP-Zeo Cassette (H2BC11 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPPEDs
Plasmid#162469Purposeprotoplast test vector of pPPEDDepositorInsertsCas9H840A
attR gateway destination cassette
UsePrime editing destination vector for transient ex…TagsMLV reverse transcriptaseExpressionPlantMutationChange histidine 840 to AlanineAvailable SinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCas12a
Plasmid#186446PurposeEncodes Cas12a from F. novicida (type V-A)DepositorInsertCas12a
UseCRISPRPromoterT7Available SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCK052 p5E exorh:mCherry
Plasmid#195953PurposeGateway p5E vector with reporterDepositorInsertexorh:mCherry
UseOtherAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD10
Plasmid#195848PurposeExpresses human FZD10 with an N-terminal HiBiTDepositorAvailable SinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSC-mCh-P2A-ST-EGFP-CAAX
Plasmid#207636PurposeA plasmid encoding photocaged SpyCatcher (pSC) fused to mCherry and a SpyTag-EGFP localized to the cell membrane via CAAX tagDepositorInsertphotocaged SpyCatcher-mCh-P2A-SpyTag-EGFP-CAAX
ExpressionMammalianMutationAmber stop codon at SpyCatcher’s critical lysinePromoterCMVAvailable SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPReporter-mCherry
Plasmid#65008PurposeE. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.DepositorInsertmCherry
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterUser-definedAvailable SinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
PDLIM4_PDZ_1
Plasmid#103941PurposeProtein expression and purification of RIL BGLII PDZ domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MBC2-null
Plasmid#162749PurposeMSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2) for expression of TCR genesDepositorInsertmurine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2)
UseRetroviralExpressionMammalianAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
MBC1-null
Plasmid#162748PurposeMSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genesDepositorInsertmurine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)
UseRetroviralExpressionMammalianAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-ShadowY-Rhotekin(8-89)-P2A-Clover(T153M/F223R)-RhoA
Plasmid#165435PurposeExpresses ShadowY-Rhotekin(8-89) and Clover(T153M/F223R)-RhoA in mammalian cells to monitor Cdc42 activity with FLIM imaging.DepositorInsertShadowY-RBD-P2A-Clover(T153M/F223R)-RhoA
TagsShadowY, Clover(T153M/F223R)ExpressionMammalianPromoterCMVAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRI006-pYFAC-riboB-PgpdA-dSpCas9-VPR-TtrpC
Plasmid#140199PurposeEpisomal expression of dSpCas9-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.DepositorInsertdSpCas9-VPR
UseCRISPR and Synthetic Biology; Expression in asper…TagsVPR (VP64-p65-Rta), NLSPromoterPgpdAAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGria2
Plasmid#124854PurposeMutagenesis of Gria2DepositorInsertGria2 (Gria2 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
JF001A
Plasmid#53116Purposeexpresses alpha hemolysin under the control of theophylline riboswitchDepositorInserttheophylline riboswitch and alpha hemolysin
ExpressionBacterialPromoterT7 promoterAvailable SinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Cre sgAmotl2 v3
Plasmid#158602Purposelenti-viral construct with Cas9, Cre recombinbase and U6 driven sgRNA against mouse Amotl2DepositorInsertAmotl2 sgRNA
ExpressionMammalianPromoterU6Available SinceSept. 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHES795
Plasmid#87943PurposeConstitutively expresses estradiol-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.DepositorInsertZif268 DBD - hER LBD - MSN2 AD (ESR1 Human, Synthetic, Budding Yeast)
ExpressionYeastPromoterADH1 (crippled)Available SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV hSyn hPGK1-mRuby
Plasmid#220911PurposeNeuronal specific expression of the fusion construct PGK1-mRubyDepositorAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV10.KH
Plasmid#63208PurposepCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Fyn-Fyn Biosensor
Plasmid#124655PurposeFRET biosensor. Fyn-tagged biosensor targeting to membraneDepositorInsertSynthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
ExpressionMammalianAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA pcDNA3
Plasmid#206051Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain IIDepositorAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 BBS pcDNA3
Plasmid#206057Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain IIDepositorAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRI004-pYFAC-riboB-PgpdA-dLbCas12a-VPR-TtrpC
Plasmid#140197PurposeEpisomal expression of dLbCas12a-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.DepositorInsertdLbCas12a-VPR
UseCRISPR and Synthetic Biology; Expression in asper…TagsVPR (VP64-p65-Rta), NLSMutationD832A DNase deactivatedPromoterPgpdAAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
PCXLE-hUL + mTAGBFP2
Plasmid#74944PurposeDosage control and tracing of reprogramming episomesDepositorAvailable SinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKM50
Plasmid#123656PurposeEncodes Renilla luciferase under the Aedes aegypti ubiquitin UbL40 promoter for constitutive expression in mosquito cellsDepositorInsertAedes aegypti UbL40 promoter
UseLuciferaseExpressionInsectPromoterUbL40 promoterAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGrin2a
Plasmid#124850PurposeMutagenesis of Grin2aDepositorInsertGrin2a (Grin2a Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD5
Plasmid#195843PurposeExpresses human FZD5 with an N-terminal HiBiTDepositorAvailable SinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-MYO1C
Plasmid#227306PurposeDonor template for mStayGold insertion into the N-terminus of the MYO1C locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MYO1C (Addgene #227305)DepositorInsertMYO1C Homology Arms flanking a mStayGold Tag (MYO1C Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCK081 desmaMCS:minpromCerulean,exorh:mCherry
Plasmid#200014PurposeExpression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, exorh:mCherryDepositorTypeEmpty backboneUseZebrafish expressionAvailable SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP18 (CK2alpha' K69M)
Plasmid#27090DepositorAvailable SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only