We narrowed to 22,191 results for: his

1. pDC20

   Plasmid

   #131366

   Purpose

   expression of MEG-3 (aa1-862) in C. elegans

   Depositor

   Geraldine Seydoux

   Article

   Smith et al Elife. 2016 Dec 3;5. pii: 21337. doi: 10

   Insert

   meg-3 (aa1-862) (meg-3 Nematode)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMCSG7-D16A-NmeCas9

   Plasmid

   #71476

   Purpose

   bacterial pMCSG7 expression vector expressing Nme cas9 nickase with a D16A mutation (RuvC domain) with T7 promoter, N-terminal His tag and TEV site

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

   Insert

   pMCSG7-D16A-NmeCas9
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   D16A

   Promoter

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Ac5-STABLE2-blast

   Plasmid

   #208034

   Purpose

   Enables expression of 2 ORFs (with or without tags) and selection with blasticidin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-blast with Actin5C promoter.

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMCSG7-H588A-NmeCas9

   Plasmid

   #71477

   Purpose

   bacterial pMCSG7 expression vector expressing Nme cas9 nickase with H588A mutation (HNH domain) with T7 promoter, N-terminal His tag and TEV site

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

   Insert

   pMCSG7-H588A-NmeCas9
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   H588A

   Promoter

   T7

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYES2.1-pomSir1

   Plasmid

   #107447

   Purpose

   Overexpression of MET fusion protein in S. cerevisiae

   Depositor

   Clare O'Connor

   Article

   Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

   Insert

   Sir1 (sir1 Fission Yeast)
   

   Use

   Tags

   V5-His

   Expression

   Yeast

   Mutation

   H523R, A750T

   Promoter

   GAL1

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEF-ENTR C (709-1)

   Plasmid

   #17429

   Purpose

   Gateway entry vector with EF1-a promoter, V5-His C-term, frame C 

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMBP-LbCas12a H759A

   Plasmid

   #113518

   Purpose

   Expresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processing

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   LbCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   H759A

   Promoter

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CBP-HAT-pET

   Plasmid

   #21091

   Depositor

   Corey Largman

   Article

   Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

   Insert

   CBP (Crebbp Mouse)
   

   Use

   Tags

   His and T7 tag

   Expression

   Bacterial

   Mutation

   HAT domain aa 1171-1621

   Promoter

   Available Since

   Aug. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pET21c-IF3mt

   Plasmid

   #31078

   Depositor

   Linda Spremulli

   Article

   Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

   Insert

   Mature form human mitochondrial translational initiation factor 3 (MTIF3 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Includes coding sequence for the mature form. Ami…

   Promoter

   Available Since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pDEST17-Nanog

    Plasmid

    #13988

    Depositor

    Shinya Yamanaka

    Article

    Mitsui et al Cell. 2003 May 30. 113(5):631-42.

    Insert

    Nanog (Nanog Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pCDB328

    Plasmid

    #113672

    Purpose

    expresses Ulp1_R4 in Escherichia coli

    Depositor

    Christopher Bahl

    Article

    Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

    Insert

    Ulp1_R4
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    contains solubility enhancing mutations

    Promoter

    T7

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSB_init_Sb92

    Plasmid

    #159424

    Purpose

    Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb92

    Depositor

    Dianfan Li

    Article

    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

    Insert

    Sb92
    

    Use

    Tags

    c-Myc and poly-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSB_init_Sb68

    Plasmid

    #159423

    Purpose

    Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb68

    Depositor

    Dianfan Li

    Article

    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

    Insert

    Sb68
    

    Use

    Tags

    c-Myc and poly-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET15-MTFmt

    Plasmid

    #31174

    Depositor

    Linda Spremulli

    Article

    Takeuchi et al J Biol Chem. 1998 Jun 12. 273(24):15085-

    Insert

    Mature form bovine mitochondrial methionyl-tRNA transformylase (MTFMT Bovine)
    

    Use

    Tags

    His and thrombin cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3-FRB-LplA(W37V)

    Plasmid

    #35608

    Depositor

    Alice Ting

    Article

    Slavoff et al J Am Chem Soc. 2011 Dec 14;133(49):19769

    Insert

    FRB-LplA(W37V)
    

    Use

    Tags

    FLAG (internal fusion), HA, and His Tag

    Expression

    Mammalian

    Mutation

    LplA has Trp37 to Val mutation

    Promoter

    CMV

    Available Since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYTSG

    Plasmid

    #159419

    Purpose

    Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Saccharomyces cerevisiae

    Depositor

    Dianfan Li

    Article

    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

    Insert

    thermostable GFP (TGP)
    

    Use

    Tags

    polyhistidine (poly-His)

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEF-ENTR B (708-1)

    Plasmid

    #17428

    Purpose

    Gateway entry vector with EF1-a promoter, V5-His C-term, frame B

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. tISR

    Plasmid

    #122664

    Purpose

    Express tISR protein in E. coli

    Depositor

    Robert Cohen

    Article

    Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Insert

    tISR (OCLM Human)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    July 17, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pETM41-EcPPXc

    Plasmid

    #38329

    Depositor

    Florian Freimoser

    Article

    Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

    Insert

    E. coli polyphosphatase (ppx Escherichia coli)
    

    Use

    Tags

    His, MBP, and TEV

    Expression

    Bacterial

    Mutation

    C-terminus of E. coli polyphosphatase (described …

    Promoter

    T7

    Available Since

    Aug. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pAC-Cyc

    Plasmid

    #33156

    Depositor

    Michael Rosbash

    Article

    Kadener et al PLoS Biol. 2008 May 20;6(5):e119.

    Insert

    Cyc (cyc Fly)
    

    Use

    Retroviral

    Tags

    6xHIS and V5

    Expression

    Insect

    Mutation

    Promoter

    Actin 5C

    Available Since

    Dec. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

21. pET-DUET-1-mRhubarb719-HO

    Plasmid

    #141200

    Purpose

    Expresses mRhubarb719 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb719
    heme oxygenase
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

    Promoter

    T7

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pETDuet RagA-RagC(S75N)

    Plasmid

    #99645

    Purpose

    Bacterial expression of RagA-RagC(S75N)

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    S75N

    Promoter

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBMFB2

    Plasmid

    #31278

    Depositor

    Aziz Sancar

    Article

    Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

    Insert

    Bmal1 (Bmal1 Mouse)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

24. pMS33: pTwinStrep-SUMO-PmcTnsD

    Plasmid

    #168166

    Purpose

    PmcTnsD expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    PmcCAST TnsD
    

    Use

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKM394

    Plasmid

    #134211

    Purpose

    Baculovirus expression of CENP-N and CENP-L

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    CENP-N-his-CENP-L
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSB_init-Sb66

    Plasmid

    #159422

    Purpose

    Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb66

    Depositor

    Dianfan Li

    Article

    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

    Insert

    Sb66
    

    Use

    Tags

    c-Myc and poly-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. E17TAG GFP zeo resistance

    Plasmid

    #69118

    Purpose

    For E17TAG GFP reporter expression in non-zeocin resistant strains

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    E17TAG GFP
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    E17 to TAG

    Promoter

    Available Since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pST-KNarK2

    Plasmid

    #44565

    Purpose

    for ectopic hypoxia inducible expression of mycobacterial proteins

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. K560CLM T324C

    Plasmid

    #24460

    Depositor

    Ron Vale

    Article

    Yildiz et al Science. 2004 Jan 30. 303(5658):676-8.

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S T324C/C421A

    Promoter

    Available Since

    Aug. 12, 2010

    Availability

    Academic Institutions and Nonprofits only

30. CaYin1

    Plasmid

    #33067

    Depositor

    Robert Campbell

    Article

    Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

    Insert

    CaYin1
    

    Use

    Tags

    6 His tag

    Expression

    Bacterial

    Mutation

    Promoter

    ARA promoter

    Available Since

    Jan. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCellFree_G03 PDGFRB

    Plasmid

    #67130

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PDGFRB (PDGFRB Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pKM463

    Plasmid

    #134214

    Purpose

    Baculovirus expression of CENP-U and CENP-Q

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    his-CENP-U + Q
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. HIV-1 integrase F185H/C280S in pET15b

    Plasmid

    #61672

    Purpose

    HIV-1 integrase F185H/C280S in pET-15b

    Depositor

    Robert Craigie

    Article

    Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

    Insert

    HIV-1 integrase F185H/C280S
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Mutation

    Promoter

    T7 promotor

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

34. pCellFree_G03 NFX1

    Plasmid

    #67131

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    NFX1 (NFX1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

35. pFTSG

    Plasmid

    #162389

    Purpose

    Expression of proteins with a C-terminal thermostable GFP (TGP) tag in insect cell

    Depositor

    Dianfan Li

    Article

    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

    Insert

    thermostable GFP (TGP)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. f2H ∂HD (272)

    Plasmid

    #24428

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al Neoplasia. 2005 Nov . 7(11):1001-10.

    Insert

    SULF2 (SULF2 Human)
    

    Use

    Tags

    FLAG, His, and Myc

    Expression

    Mammalian

    Mutation

    AA 418-716 deleted, 23AA N-terminal truncation

    Promoter

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

37. GRLF1

    Plasmid

    #36195

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    GRLF1 (Codon Devices Synthesized) (ARHGAP35 Human)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    Contains the Ras homolog domain only (amino acid …

    Promoter

    Available Since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

38. pJCSUP35(1-122)

    Plasmid

    #1091

    Depositor

    Susan Lindquist

    Article

    Glover et al Cell 1997 May 30;89(5):811-9.

    Insert

    SUP35 (SUP35 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    amino acids 1 - 122 of SUP35

    Promoter

    Available Since

    Jan. 21, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAcUW51-gE2

    Plasmid

    #11621

    Depositor

    Pamela Bjorkman

    Article

    Sprague et al J Biol Chem. 2004 Apr 2. 279(14):14184-9

    Insert

    HSV-1 glycoprotein E
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    deleted amino acids 391-550

    Promoter

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

40. TrmD_HaeIn/pET28b(+) N,C-tag

    Plasmid

    #12665

    Depositor

    SeWon Suh

    Article

    Kim et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    tRNA (guanine-N(1)-)-methyltransferase (trmD Haemophilus influenzae)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge