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pOsU3-esgRNA

Plasmid

#115629

Purpose

expression esgRNA in rice protoplasts

Depositor

Caixia Gao

Article

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

OsU3p-esgRNA

Use

CRISPR

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pWZL Hygro- TRF2

Plasmid

#18009

Depositor

Titia de Lange

Article

Karlseder et al Science. 2002 Mar 29. 295(5564):2446-9.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

aa2-500

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
p12xEGFP-N1

Plasmid

#122172

Purpose

Expression of 12xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

12xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pKER-mRuby2

Plasmid

#85977

Purpose

Expresses mRuby2 from the EF1a promoter in mammalian cells

Depositor

Michele Calos

Article

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

mRuby2

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pSG 12-6

Plasmid

#63870

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UAG near 5' end

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pSG 627

Plasmid

#63872

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UAA near 5' end

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pTGFP-T7-Hha10comp

Plasmid

#65428

Purpose

Competitor vector for transcriptional mutagenesis assay

Depositor

Yinsheng Wang

Article

You et al Nat Protoc. 2015 Sep;10(9):1389-406. doi

Type

Empty backbone

Expression

Mammalian

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pSG 12DP

Plasmid

#63876

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

-1 frameshift near 5' end

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pSG 34-11

Plasmid

#63869

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UGA near N-term

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
promoter-less mRFP1E

Plasmid

#183144

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1E

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSG lacZ

Plasmid

#63874

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

+1 frameshift near 5' end

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pAW-SpyCsn2

Plasmid

#113251

Purpose

E. coli expression vector for S. pyogenes Csn2-MBP-His

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Csn2

Tags

MBP-6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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p10xEGFP-N1

Plasmid

#122170

Purpose

Expression of 10xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

10xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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p5426

Plasmid

#168187

Purpose

bacteria reporter

Depositor

Michael Hensel

Article

Schulte et al Commun Biol. 2021 May 4;4(1):520. doi: 1

Insert

PBAD::dsred PtetA::sfgfp

Expression

Bacterial

Mutation

none

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSG 3/4

Plasmid

#63868

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UGA near N-term

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pSG 853

Plasmid

#63873

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UAA near 5' end

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pENTR1a-jAspSnFR3-mRuby3

Plasmid

#203491

Purpose

jAspSnFR3-mRuby3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3

Use

Gateway entry vector

Tags

His-tag and mRuby3

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pSG 163

Plasmid

#63871

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UAG near 5' end

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
p5xEGFP-N1

Plasmid

#122165

Purpose

Expression of 5xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

5xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAW-SpyCas1

Plasmid

#113248

Purpose

E. coli expression vector for His-MBP-S. pyogenes Cas1

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Cas1

Tags

6xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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