We narrowed to 16,282 results for: form
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Plasmid#28262DepositorAvailable SinceApril 11, 2011AvailabilityAcademic Institutions and Nonprofits only
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MD~E-pCLBabe
Plasmid#20912DepositorInsertMyoD~E12 forced dimer
UseRetroviralTagsFlagExpressionMammalianMutationShort flexible linker region between the MyoD and…Available SinceNov. 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
p8327 pLIX YAP1 S127A
Plasmid#184528PurposeExpression of YAP1 S127ADepositorAvailable SinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-SEPT2_SEPT6
Plasmid#174497Purposebacterial co-expression of human SEPT2 and of human SEPT6DepositorAvailable SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-RRAR/D614G
Plasmid#164570PurposeSARS-CoV-2 Spike protein with D614G mutation and Furin site restored (S-RRAR-D614G variant)DepositorInsertSpike (S-RRAR-D614G variant)
Tags2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…ExpressionMammalianMutationD614GAvailable SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRG646_lexO-HO_LexA-TF_LEU2MX
Plasmid#154815PurposeInducible expression of HO endonuclease in S. cerevisiaeDepositorInsertsTagsFLAGExpressionYeastPromoterACT1 and lexO_4-CYC1_coreAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS
Plasmid#107320PurposeExpresses SpCas9 fused to SaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 is fused to SaCas9PromoterCMV IE94Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)1x
Plasmid#112055PurposeACTB mRNA tagged with 1 copy of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
TagsRiboglow RNA tagExpressionMammalianMutation1 copy of Riboglow tag (variant A) after stop cod…PromoterCMVAvailable SinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
FKBP-alpha(740-977)-GFP
Plasmid#100731PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosis (negative control)DepositorInsertAP2A1 (Ap2a1 Mouse)
TagsFKBP and GFPExpressionMammalianMutationLong isoformPromoterCMVAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF3448
Plasmid#144924PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+36)
Plasmid#199165Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+36)
TagsMGHHHHHHGGAMutationE16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…PromoterT7Available SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-pHSE::Cre-mRuby3
Plasmid#164136PurposeLentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of heat inductionDepositorInsertHSE promoter-Cre-T2A-mRuby3
UseCRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…ExpressionMammalianPromoterHSE synthetic promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP/Venus-mULK1
Plasmid#58743PurposeExpresses Venus-mULK1 in mammalian cellsDepositorInserta novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
UseRetroviralTagsVenusAvailable SinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 βarr1 S412A Flag
Plasmid#42195DepositorAvailable SinceJan. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293
Plasmid#118412PurposeExpresses firefly luciferase under the control of a strong ubiquitous promoter EF1a along with a WPRE cassette. Backbone is compatible with both baculoviral and human production methods of AAV.DepositorInsertFirefly luciferase
UseAAV, Luciferase, and Synthetic BiologyExpressionInsect and MammalianPromoterEF1aAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2xNLS-SpCas9MT3-NLS-3xHA-NLS-dSaCas9-2xNLS
Plasmid#107318PurposeExpresses SpCas9 (R1335K) fused to dSaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 (R1335K) fused to nuclease dead SaCas9 (D1…PromoterCMV IE94Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLXSN-EGFR
Plasmid#65226PurposeExpression of EGFR in mammalian cellsDepositorAvailable SinceOct. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBV-Luc/Del-7
Plasmid#14968DepositorInsertc-myc promoter (-26/+334) (MYC Human)
UseLuciferaseExpressionMammalianMutation-26/+334 (relative to the P2 transcription initia…Available SinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTH726-CEN-RLuc/minCFLuc
Plasmid#38210DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3 (=GPD)Available SinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAaePUb::Cas9-2A-Neo
Plasmid#176680PurposeExpression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888)DepositorInsertspCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
UseCrisprExpressionInsectMutationDrosophila codon optimizedPromoterAe. aegypti poly-ubiquitin (AAEL003888)Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only