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pFA6a-link-yoLSS-mKate2-Kan

Plasmid

#44943

Purpose

Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoLSS-mKate2

Expression

Yeast

Available Since

Oct. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2+cPLA2-mKate2

Plasmid

#162068

Purpose

Used to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.

Depositor

Philipp Niethammer

Article

Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

Insert

cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Use

Avian, xenopus, zebrafish

Tags

mKate2

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pFGAMS-mCherry

Plasmid

#166024

Purpose

Expresses human PFAS-mCherry fusion in mammalian cells

Depositor

Stephen Benkovic

Article

Doigneaux et al J Biol Chem. 2020 Jul 10;295(28):9551-95

Insert

PFAS-mCherry (PFAS Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pClathrin-miRFP670nano

Plasmid

#127430

Purpose

Clathrin labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

miRFP670nano-clathrin light chain

Tags

miRFP670nano

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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Launchpad (AVA2590)

Plasmid

#85442

Purpose

Lentiviral FRT-flanked EF1alfa-GFP PGK-PuromycinR cassette

Depositor

Joan Steitz

Article

Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

Insert

FRT-flanked EF1alfa-GFP PGK-Puromycin cassette

Use

Lentiviral

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only
PB-REVERBA-mScarlet-NLS-PEST

Plasmid

#190658

Purpose

Fluorescent reporter for circadian rhythms

Depositor

Mary Teruel

Article

Zhang et al Proc Natl Acad Sci U S A. 2022 Aug 16;11

Insert

Reverba-mScarlet (Nr1d1 Mouse)

Expression

Mammalian

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)

Plasmid

#138555

Purpose

Expresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

eSpCas9(1.1)

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

K848A, K1003A, R1060A

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAH123

Plasmid

#66077

Purpose

The pAH123 plasmid [Haldimann2001] is the phi80 helper plasmid necessary for the genome integration of the burden monitor phi80 version

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

Insert

phi80 integrase

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pMB22

Plasmid

#122244

Purpose

Lentivirus delivery for stable expression of AsCas12a-VPR

Depositor

Darjus Tschaharganeh

Article

Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

Insert

mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator

Use

Lentiviral

Tags

NLS and VPR activator

Promoter

EFs

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pBLADE(FP6*)-mCherry

Plasmid

#168048

Purpose

Expresses the synthetic gene BLADE FP6 under the constitutive J23101* promoter. mCherry expression is controlled by the PBAD promoter.

Depositor

Barbara Di Ventura

Article

Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

Inserts

Blue light-inducible AraC dimers in Escherichia coli
mCherry

Expression

Bacterial

Promoter

J23101* and PBAD

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-UBC-S1-GCaMP6f

Plasmid

#160722

Purpose

Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.

Depositor

Edward Boyden

Article

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

S1-GCaMP6f

Use

AAV

Expression

Mammalian

Mutation

N/A

Promoter

Human ubiquitin C (UBC) promoter

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pKD239-1g-2

Plasmid

#90959

Purpose

Expresses S. baltica TtrR and mCherry constitutively and sfGFP under the PttrB185-269 promoter

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Inserts

Tetrathionate response regulator
Superfolder GFP
mCherry

Expression

Bacterial

Promoter

Bba_J23100, Bba_J23109, and PttrB185-269

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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P2-ECFP-pA (Construct 6)

Plasmid

#55198

Purpose

Synthetic promoter responsive to gRNA2

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

mCerulean

Use

Synthetic Biology

Expression

Mammalian

Promoter

Minimal Ade MLP

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pXylA-agrCA-I

Plasmid

#53437

Purpose

xylose-dependent expression of the type-I agrC and agrA genes of S. aureus

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrCA locus

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

"MVQTS" sequence added to the N-terminu…

Promoter

PxylA

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pBA2kIKmA2

Plasmid

#39213

Depositor

Anastasios Melis

Article

Lindberg et al Metab Eng. 2010 Jan;12(1):70-9. Epub 200

Insert

Kudzu Isoprene Synthase

Use

Synthetic Biology

Mutation

Deleted the predicted chloroplast transit peptide

Promoter

psbA2

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pSB-CMV-MCS-Puro cPLA2-mK2-P2A-EGFP-LaminB1

Plasmid

#162566

Purpose

Used for the dual color labeling of cPLA2 and LaminB1 expression.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Inserts

cytosolic phospholipase a2 (pla2g4aa Zebrafish)
Lamin B1 (lmnb1 Zebrafish)

Tags

EGFP, P2A, and mKate2

Expression

Mammalian

Promoter

CMV and SV40

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRosa26-4xInsulator (JP6)

Plasmid

#99627

Purpose

IfgMosaic acceptor vector for Classical or Cas9 Gene targeting of the Rosa26 locus

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Gt(Rosa)26Sor (Gt(ROSA)26Sor Mouse)

Use

Mouse Targeting

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pICH41800

Plasmid

#48020

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pICH41822

Plasmid

#48021

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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FASTHDR-tRNApromo-sgRNA-espCas91_1

Plasmid

#167210

Purpose

Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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