We narrowed to 4,589 results for: LUM

1. GB1-His-TET1-878-911-T898E(pL91)

   Plasmid

   #124394

   Purpose

   E.Coli produced TET1-SID mutants (T898E) purified by FPLC nickel column

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   GB1-His-TET1-878-911-T898E (TET1 Human)
   

   Use

   Tags

   GB1-Histidine

   Expression

   Bacterial

   Mutation

   T898E

   Promoter

   Available since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. GB1-His-TET1-878-911-T898A(pL91)

   Plasmid

   #124393

   Purpose

   E.Coli produced TET1-SID mutants (T898A) purified by FPLC nickel column

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   GB1-His-TET1-878-911-T898A (TET1 Human)
   

   Use

   Tags

   GB1-Histidine

   Expression

   Bacterial

   Mutation

   T898A

   Promoter

   Available since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. GB1-His-TET1-878-911-L897A(pL91)

   Plasmid

   #124392

   Purpose

   E.Coli produced TET1-SID mutants (L897A) purified by FPLC nickel column

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   GB1-His-TET1-878-911-L897A (TET1 Human)
   

   Use

   Tags

   GB1-Histidine

   Expression

   Bacterial

   Mutation

   L897A

   Promoter

   Available since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. GB1-His-TET1-878-911-I894A(pL91)

   Plasmid

   #124391

   Purpose

   E.Coli produced TET1-SID mutants (I894A) purified by FPLC nickel column

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   GB1-His-TET1-878-911-I894A (TET1 Human)
   

   Use

   Tags

   GB1-Histidine

   Expression

   Bacterial

   Mutation

   I894A

   Promoter

   Available since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF3311

   Plasmid

   #144787

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CREB3 (CREB3 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEmerald-Sec61b-C1

   Plasmid

   #90992

   Purpose

   Expresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

   Insert

   mEmerald-Sec61b (SEC61B Synthetic, Human)
   

   Use

   Tags

   mEmerald (green fluorescence)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-Sec61b-C1

   Plasmid

   #90994

   Purpose

   Expresses mCherry-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

   Insert

   mCherry-Sec61b (SEC61B Synthetic, Human)
   

   Use

   Tags

   mCherry (red fluorescence)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. MCS-3_Endofin_LgBiT

   Plasmid

   #182574

   Purpose

   This plasmid can be used to monitor receptor internalization in real time living cells using bioluminescence as a readout.

   Depositor

   Bradley McConnell

   Article

   Reyes-Alcaraz et al Commun Biol. 2022 Mar 8;5(1):212. doi: 1

   Insert

   zinc finger FYVE-type (ZFYVE16 Human)
   

   Use

   Tags

   LgBiT (Large BiT)

   Expression

   Mammalian

   Mutation

   FYVE domain Q739-K806

   Promoter

   Herpes simplex virus (HSV)

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. mApple-Sec61b-C1

   Plasmid

   #90993

   Purpose

   Expresses mApple-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

   Insert

   mApple-Sec61b (SEC61B Synthetic, Human)
   

   Use

   Tags

   mApple (red fluorescence)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR

    Plasmid

    #207805

    Purpose

    E-NoMi is a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins under an EF-1α promoter.

    Depositor

    Koen Breyne

    Article

    Maalouf et al Mol Ther. 2023 Jul 5;31(7):2206-2219. do

    Insert

    E-NoMi
    

    Use

    Lentiviral

    Tags

    3xFlag tag, copGFP, mCherry, and nanoluciferase

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONRp5-p2 SspB-mCherry-CShroom3

    Plasmid

    #170977

    Purpose

    C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.

    Depositor

    Miki Ebisuya

    Article

    Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

    Insert

    CShroom3 (Shroom3 Mouse)
    

    Use

    Gateway cloning

    Tags

    SspB and mCherry

    Expression

    Mutation

    Isoform X2, deleted aminoacids 1 - 1388

    Promoter

    Available since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUAS-Myc-BirA-G3-ER

    Plasmid

    #173815

    Purpose

    Promiscuous biotin ligase (BirA-G3) targeted to ER lumen expressed from UAS enhancer. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site

    Depositor

    Norbert Perrimon

    Article

    Droujinine et al Nat Commun. 2021 Apr 22;12(1):2382. doi:

    Insert

    Myc-BirA-G3-ER
    

    Use

    Tags

    Myc

    Expression

    Insect

    Mutation

    Promoter

    UAS

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. mIFP-Calnexin-N-14

    Plasmid

    #56214

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    Calnexin (CANX Human)
    

    Use

    Tags

    mIFP

    Expression

    Mammalian

    Mutation

    I474N in Calnexin

    Promoter

    CMV

    Available since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. MEF2A-Sensor

    Plasmid

    #208928

    Purpose

    in vitro MAPK-Sensor for luminescent detection of ERK and p38-activity

    Depositor

    Attila Reményi

    Article

    Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

    Insert

    Mef2a docking sequence+Phospho site (MEF2A Human)
    

    Use

    Tags

    HISx6, MBP, and Smbit

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCIBN-hTRF2-tagRFP-T

    Plasmid

    #103812

    Purpose

    BLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-hTRF2-tagRFP-T (CIB1, TERF2 Human, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hTRF2: deletion of amino acids 1-42 and 476 compa…

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCardinal-Calnexin-N-14

    Plasmid

    #56154

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    Calnexin (CANX Human)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    I474N in Calnexin

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pCIBN-tagBFP-hNCL

    Plasmid

    #103805

    Purpose

    BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-tagBFP-hNCL (CIB1, NCL Human, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCIBN-LacI

    Plasmid

    #103814

    Purpose

    BLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-LacI (CIB1, lacI E. coli, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    LacI: C19T (silent, L7L), T264C (silent, A88A), C…

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCIBN-EYFP-hNCL

    Plasmid

    #103806

    Purpose

    BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-EYFP-hNCL (CIB1, NCL Human, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGECPL.BC10.MG

    Plasmid

    #223822

    Purpose

    Contains Cas9-editable barcode, marking guide (MG) for lineage tracing, Cre for Cas9 activation and loxP-mediated gene deletion, and Firefly Luciferase (FLuc) for live luminescence-based monitoring.

    Depositor

    Dawid Nowak

    Article

    Serio et al Cancer Discov. 2024 Jul 5. doi: 10.1158/

    Insert

    Ef1a-Driven Cre-P2A-FLuc
    

    Use

    CRISPR, Cre/Lox, Lentiviral, Luciferase, and Mous…

    Tags

    P2A

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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