We narrowed to 17,934 results for: multi
-
Plasmid#69344PurposeSQ171 fast growing strain supported by pCSacB plasmidDepositorInsertpCSacB
Available sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {mCAR}off-{DTR-GFP}on-WPRE
Plasmid#111395PurposeAAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON DTR-EGFP (diphtheria toxin receptor for cell ablation)DepositorInsertsUseAAVTagsEGFP and MycExpressionMammalianPromoterhSynapsinAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoLSS-mKate2-Kan
Plasmid#44943PurposeYeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with Kan selectionDepositorInsertyoLSS-mKate2
ExpressionYeastAvailable sinceOct. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2+cPLA2-mKate2
Plasmid#162068PurposeUsed to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseAvian, xenopus, zebrafishTagsmKate2ExpressionMammalianPromoterCMVAvailable sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFGAMS-mCherry
Plasmid#166024PurposeExpresses human PFAS-mCherry fusion in mammalian cellsDepositorAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pClathrin-miRFP670nano
Plasmid#127430PurposeClathrin labeling with near-infrared fluorescent protein miRFP670nanoDepositorInsertmiRFP670nano-clathrin light chain
TagsmiRFP670nanoExpressionMammalianPromoterCMVAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Launchpad (AVA2590)
Plasmid#85442PurposeLentiviral FRT-flanked EF1alfa-GFP PGK-PuromycinR cassetteDepositorInsertFRT-flanked EF1alfa-GFP PGK-Puromycin cassette
UseLentiviralAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lenti-EF1alpha-dCas9-p300_Blast
Plasmid#192653Purpose3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance markerDepositorInsertdCas9-p300
UseCRISPR and LentiviralExpressionMammalianMutationD10A; H840A in Cas9PromoterEF1aAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-REVERBA-mScarlet-NLS-PEST
Plasmid#190658PurposeFluorescent reporter for circadian rhythmsDepositorInsertReverba-mScarlet (Nr1d1 Mouse)
ExpressionMammalianAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
eSpCas9(1.1)
Plasmid#138555PurposeExpresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSDDepositorInserteSpCas9(1.1)
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationK848A, K1003A, R1060APromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAH123
Plasmid#66077PurposeThe pAH123 plasmid [Haldimann2001] is the phi80 helper plasmid necessary for the genome integration of the burden monitor phi80 versionDepositorInsertphi80 integrase
Available sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMB22
Plasmid#122244PurposeLentivirus delivery for stable expression of AsCas12a-VPRDepositorInsertmammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator
UseLentiviralTagsNLS and VPR activatorPromoterEFsAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBLADE(FP6*)-mCherry
Plasmid#168048PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101* promoter. mCherry expression is controlled by the PBAD promoter.DepositorInsertsBlue light-inducible AraC dimers in Escherichia coli
mCherry
ExpressionBacterialPromoterJ23101* and PBADAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S1-GCaMP6f
Plasmid#160722PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.DepositorInsertS1-GCaMP6f
UseAAVExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKD239-1g-2
Plasmid#90959PurposeExpresses S. baltica TtrR and mCherry constitutively and sfGFP under the PttrB185-269 promoterDepositorInsertsTetrathionate response regulator
Superfolder GFP
mCherry
ExpressionBacterialPromoterBba_J23100, Bba_J23109, and PttrB185-269Available sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
P2-ECFP-pA (Construct 6)
Plasmid#55198PurposeSynthetic promoter responsive to gRNA2DepositorInsertmCerulean
UseSynthetic BiologyExpressionMammalianPromoterMinimal Ade MLPAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pXylA-agrCA-I
Plasmid#53437Purposexylose-dependent expression of the type-I agrC and agrA genes of S. aureusDepositorInsertagrCA locus
UseSynthetic BiologyTagsnoneExpressionBacterialMutation"MVQTS" sequence added to the N-terminu…PromoterPxylAAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBA2kIKmA2
Plasmid#39213DepositorInsertKudzu Isoprene Synthase
UseSynthetic BiologyMutationDeleted the predicted chloroplast transit peptidePromoterpsbA2Available sinceAug. 16, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMBEC2
Plasmid#187596PurposeBase editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Kanamycin resistanceDepositorInsertnCas9-BEC-UGI; cas6f, gfp
ExpressionBacterialPromotertrcAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only