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pSLIK 3xHA-MKK6-EE neo

Plasmid

#47546

Purpose

Lentiviral expression vector for an inducible 3xHA-tagged constitutively active MKK6 mutant

Depositor

Kevin Janes

Publication

Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

Insert

MKK6-EE (MAP2K6 Human)

Use

Lentiviral

Tags

3XHA

Expression

Mammalian

Mutation

S207E, T211E

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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YFP-Cdc42(N17)

Plasmid

#11400

Depositor

Joel Swanson

Publication

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

cell division cycle 42 (CDC42 Human)

Tags

YFP

Expression

Mammalian

Mutation

Obtained with Threonine 17 changed to Asparagine.

Available since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEBTet_NAD-Snifit-cyto

Plasmid

#113914

Purpose

Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

Depositor

Kai Johnsson

Publication

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NAD-Snifit

Expression

Mammalian

Promoter

CMV TetO2

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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IQGAP1_pLX307

Plasmid

#98346

Purpose

Lentiviral expression of IQGAP1

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

IQGAP1 (IQGAP1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-CDK6

Plasmid

#116725

Purpose

Lentiviral expression of CDK6

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

CDK6 (CDK6 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCFJ355 - MCS(ttTi14024, X)

Plasmid

#34870

Depositor

Erik Jorgensen

Publication

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

ttTi14024 targeting

Use

Worm targeting

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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E.coli RaPID plasmid

Plasmid

#107251

Purpose

Expresses the E.coli RaPID labeling protein

Depositor

Paul Khavari

Publication

Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

Insert

E.coli BirA*

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBBR1k-con-GFPuv

Plasmid

#106386

Purpose

Constitutive trc promoter expressing gfpuv on BBR1 origin

Depositor

Brian Pfleger

Publication

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

trc

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-CASK

Plasmid

#23470

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CASK (CASK Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pHBS1389 IBB-GFP-mCherry3E

Plasmid

#118803

Purpose

Fluorescence-based TDP43-dependent splicing reporter

Depositor

Rajat Rohatgi

Publication

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

Synthetic

Expression

Mammalian

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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CoV2-N-S202R

Plasmid

#177950

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S202R). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Publication

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (S202R) (N )

Expression

Mammalian

Mutation

S202R

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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spNW100

Plasmid

#173488

Purpose

Expresses spNW100 in Bl21

Depositor

Huan Bao

Publication

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW100

Tags

Histag

Expression

Bacterial

Mutation

P1049L- please see depositor comments

Promoter

T7

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-FRB-PafA

Plasmid

#113404

Purpose

Expresses FRB-PafA in bacteria

Depositor

Min Zhuang

Publication

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

FRB-PafA

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-BCCP-PupE

Plasmid

#113403

Purpose

Expresses BCCP-PupE in bacteria

Depositor

Min Zhuang

Publication

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

BCCP-PupE

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE DN Cullin3

Plasmid

#41913

Depositor

Stephen Elledge

Publication

Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

Insert

Cullin 3 (CUL3 Human)

Use

Lentiviral

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDEST47-SAR1-GFP

Plasmid

#67409

Purpose

Mammalian expression of hSar1 with C-term GFP tag

Depositor

Richard Kahn

Publication

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SAR1 (SAR1A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pOTTC293 - pAAV EF1a V5-synuclein (WT)

Plasmid

#60057

Purpose

An AAV packaging vector that expresses wildtype alpha-synuclein under control of the EF1a promoter.

Depositor

Brandon Harvey

Publication

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

alpha-synuclein (SNCA Human, human)

Use

AAV

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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SPYtag-GFPnanobody

Plasmid

#214837

Purpose

This plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

Depositor

Hironori Funabiki

Publication

Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

Insert

SPYtag-GFPnanobody

Tags

His-TEV-SUMO-

Expression

Bacterial

Promoter

T5

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EHD2-mCherry

Plasmid

#45933

Purpose

caveolar protein, confines caveolae to the plasma membrane

Depositor

Ari Helenius

Publication

Stoeber et al EMBO J. 2012 May 16;31(10):2350-64. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Eps-15 homology domain-containing protein2 (EHD2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223-NEK2

Plasmid

#23658

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK2 (NEK2 Human)

Use

Gateway Cloning

Mutation

Insert encodes truncated protein relative to NP_0…

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article