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pWN086

Plasmid

#233859

Purpose

MoClo-YTK; ConLS/R1 cassette; Auxin systems; pTDH3-AmtR-NES-TIR1-U2-tADH1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-AmtR-NES-TIR1-U2-tADH1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN113

Plasmid

#233862

Purpose

MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NES-linker-NS3a-tADH1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-LexA-NES-linker-NS3a-tADH1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-Msp4

Plasmid

#238037

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

lac

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-Msp1

Plasmid

#238034

Purpose

Encodes sfGFPunder lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

lac

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-Msp2

Plasmid

#238035

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

lac

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-Msp3

Plasmid

#238036

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

lac

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-Msp6

Plasmid

#238039

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

lac

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-Msp5

Plasmid

#238038

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

lac

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-BFP

Plasmid

#227505

Purpose

Backbone for CARGO cloning, with U6 promoter and gRNA scaffold, TagBFP, and Kanamycin resistence

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF9-Pct3-(–)-Eldh2(cHis6)-Accs(ElA2)

Plasmid

#233868

Purpose

Expresses His-tagged Eggerthella lenta A2 (–)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eldh2

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT384: pSC101-Donor(ZooTsy)

Plasmid

#200875

Purpose

ZooTsy donor harboring KanR cargo.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT368: pSC101-Donor(AjTn6022)

Plasmid

#200864

Purpose

AjTn6022 donor harboring KanR cargo.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT91: pR6K-Donor(AjTn6022)

Plasmid

#200863

Purpose

AjTn6022 donor harboring KanR cargo.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN16-HcdhA(cHis8)BC-partial cluster(ElA2)

Plasmid

#225328

Purpose

Expresses Eggerthella lenta A2 Hcdh partial gene cluster (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Hcdh partial gene cluster

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN6-Elen0497-ElVadh-noAccs(ElDSM2243)

Plasmid

#225314

Purpose

Expresses Eggerthella lenta DSM 2243 El Vadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

El Vadh (Elen_0497 Eggerthella lenta)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF25-Pct3-(–)-Eddh(nHis6)(G3C)

Plasmid

#233873

Purpose

Expresses His-tagged Gordonibacter pamelaeae 3C (–)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eddh

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF30-DadhABC(cHis8)RS-Accs(Cadh)(ElA2)

Plasmid

#233876

Purpose

Expresses Eggerthella lenta A2 Dadh with the accessory genes from the cadh gene cluster in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW-RUSH-Strep-KDEL

Plasmid

#228527

Purpose

Lentiviral vector, cloning vector for Strep-KDEL-IRES in mammalian cells.

Depositor

Ginny Farias

Article

Li et al Nat Commun. 2024 Dec;15: 10829. doi: 10.

Insert

Strep-KDEL

Use

Lentiviral

Tags

Streptavidin-KDEL

Expression

Mammalian

Mutation

Promoter

Ubc

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pMVRha

Plasmid

#229553

Purpose

Broad-host range plasmid (pBBR1), contains an oriT (RP4) for conjugation in kanamycin sensitive non-model bacteria. The GOI can be cloned under the control of a rhamnose-inducible promoter using BsaI.

Depositor

Sandy Schmidt

Article

Vajente et al ACS Synth Biol. 2024 Oct 31. doi: 10.102

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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SpyCatcher-P-Domain

Plasmid

#201193

Purpose

Expresses furin P-domain genetically fused to SpyCatcher001 in bacteria

Depositor

Vesa Hytönen

Article

Lampinen et al Nanoscale Adv. 2024 Oct 9;6(24):6239-52.

Insert

SpyCatcher-P-Domain

Use

Tags

HisTag and SpyCatcher001

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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