We narrowed to 43,328 results for: cha;

1. pET21-pelB-LaG-37

   Plasmid

   #172756

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-37
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TRE-UBC-mCD59-T2A-GFP

   Plasmid

   #205451

   Purpose

   lentiviral plasmid for expression of mouse CD59

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   mCD59 (Cd59a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMpGWB402

   Plasmid

   #68667

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus 35S promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET21-pelB-LaG-41

   Plasmid

   #172757

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-41
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1 V5 His-TOPO-GluCl optalpha-mYFP L9'F (Opt α-GluClv2.0)

   Plasmid

   #47387

   Purpose

   allow pharmacologically induced silencing of electrical activity in CNS neurons upon exposure to the anthelmintic drug ivermectin

   Depositor

   Henry Lester

   Article

   Frazier et al J Biol Chem. 2013 May 29.

   Insert

   glutamate-gated chloride Channels
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Leu9 prime mutated to F (Phenylalanine in M3-M4 l…

   Promoter

   CMV

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMpGWB435

   Plasmid

   #68700

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   TagRFP

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus 35S promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKV015

   Plasmid

   #64766

   Purpose

   Recombination-Induced Tag Exchange (RITE) Cassette, HA-GFP to a T7-RFP tag, invariable V5 tag, Hygro selection

   Depositor

   Fred van Leeuwen

   Article

   Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

   Insert

   RITE cassette
   

   Use

   Rite cassette

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. polyPRM5

   Plasmid

   #175232

   Purpose

   Bacterial expression and purification, mixed with polySH3 to form condensates

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   ABL1 (ABL1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1(+) Drp1 V2 S600D

   Plasmid

   #46345

   Depositor

   David Chan

   Article

   Loson et al Mol Biol Cell. 2013 Jan 2.

   Insert

   Drp1 V2 (Dnm1l Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S600D

   Promoter

   CMV

   Available since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1(+) Drp1 V2 S600A

    Plasmid

    #46344

    Depositor

    David Chan

    Article

    Loson et al Mol Biol Cell. 2013 Jan 2.

    Insert

    Drp1 V2 (Dnm1l Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S600A

    Promoter

    CMV

    Available since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pMpGWB131

    Plasmid

    #68585

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Emerald Luc fused with PEST sequence [ELuc(PEST)]

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFUGW-ZipACR-EYFP

    Plasmid

    #88844

    Purpose

    Fast anion channelrhodopsin (current half-decay time 2-4 ms depending on voltage) for time-resolved inhibition of neuronal spiking

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    ZipACR-NotI-EYFP
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJL1-MBP-4xDQNAT

    Plasmid

    #128390

    Purpose

    In vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Maltose binding protein
    

    Use

    Tags

    4xDQNAT glycosylation tag and 6xHis tag

    Expression

    Bacterial

    Mutation

    A302T- please see depositor comment

    Promoter

    T7

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21-pelB-LaG-11

    Plasmid

    #172762

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-11
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMpGWB331

    Plasmid

    #68659

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Emerald Luc fused with PEST sequence [ELuc(PEST)]

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21-pelB-LaM-1

    Plasmid

    #172767

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-1
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-TRE-DIO-mRuby2

    Plasmid

    #99117

    Purpose

    An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mRuby2

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    DIO-mRuby2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRK5myc Rac1 wt

    Plasmid

    #37030

    Depositor

    Lawrence Kirschner

    Article

    Manchanda et al Oncogene. 2013 Jul 25;32(30):3491-9. doi

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1(+) Drp1 V2 S579D

    Plasmid

    #46343

    Depositor

    David Chan

    Article

    Loson et al Mol Biol Cell. 2013 Jan 2.

    Insert

    Drp1 V2 (Dnm1l Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S579D

    Promoter

    CMV

    Available since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pF(UG) hSyn PP-HaloTag-SYT7alpha

    Plasmid

    #179714

    Purpose

    Lentiviral expression of mouse Syt7 with N terminal HaloTag

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pF(UG) hSyn PM-SYT7alpha deltaTMD

    Plasmid

    #179720

    Purpose

    Lentiviral expression of plasma membrane-targeted mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGCNK58

    Plasmid

    #202461

    Purpose

    Plasmid for the expression of the basic region and leucine zipper domain of the Saccharomyces cerevisiae transcription factor GCN4 in bacterial cells

    Depositor

    Arthur Palmer, Stephen Harrison

    Article

    Weiss et al Nature. 1990 Oct 11;347(6293):575-8. doi

    Insert

    Residues 226-281 of Sacchromyces cerevisiae GCN4 plus an N-terminal Met-Lys extension (GCN4 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMpGWB426

    Plasmid

    #68691

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    TagRFP

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1(+) Drp1 V2 S579A

    Plasmid

    #46342

    Depositor

    David Chan

    Article

    Loson et al Mol Biol Cell. 2013 Jan 2.

    Insert

    Drp1 V2 (Dnm1l Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S579A

    Promoter

    CMV

    Available since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pET21-pelB-LaG-29

    Plasmid

    #172753

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-29
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDS240

    Plasmid

    #34966

    Purpose

    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(K533∆, high-affinity epitope).

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    K533∆, high-affinity epitope

    Promoter

    GAL1

    Available since

    March 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMpGWB316

    Plasmid

    #68644

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Nuclear localization signal-tagged tdTomato (tdTo…

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. prp8-G736E

    Plasmid

    #108450

    Purpose

    prp8-G736E in pRS313 backbone for expression in yeast

    Depositor

    Christine Guthrie

    Article

    Mayerle et al Proc Natl Acad Sci U S A. 2019 Feb 5;116

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    changed G736 to E

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMTH PKC zeta

    Plasmid

    #8414

    Depositor

    Frederic Mushinski

    Article

    Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

    Insert

    Mouse Protein Kinase C zeta (Prkcz Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

30. p40phox-PX (1-144)

    Plasmid

    #119123

    Purpose

    Bacterial expression of human phox homology (PX) domain, p40phox-PX (1-144)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    p40phox-PX (1-144)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pEF NS34a-msGFP-'S-mito

    Plasmid

    #158765

    Purpose

    Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    NS3/4a protease and msGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Flexible linkers between the protease and msGFP a…

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. NAX_CAGGS_CAS9_m4_KRAB_KanR neo

    Plasmid

    #167877

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. ShadowG-SUMO1

    Plasmid

    #175239

    Purpose

    Bacterial expression and purification, conjugatable SUMO for SUMOylation

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    SUMO1 (SUMO1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. GFP-GW182delta1

    Plasmid

    #11592

    Depositor

    Edward Chan

    Article

    Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

    Insert

    GW182 (TNRC6A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains only aa1-498; SEE DEPOSITOR NOTES BELOW

    Promoter

    Available since

    June 30, 2006

    Availability

    Academic Institutions and Nonprofits only

35. pCVL Traffic Light Reporter 1.1 (Ani target) Ef1a Puro

    Plasmid

    #31480

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Traffic Light Reporter 1.1 (ani target)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    mCherry has M9S, M16L to reduce background mCherr…

    Promoter

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRK5 CASTOR2-HA Cterm

    Plasmid

    #84489

    Purpose

    overexpression of CASTOR2 with HA tag

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR2 (GATSL2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

37. pSB700 lenti gRNA KanR neo

    Plasmid

    #167909

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAS3-lim-4P_gtACR2_SL2_eGFP(65C)_unc-54 3'UTR

    Plasmid

    #195853

    Purpose

    Expresses inhibitory opsin gtACR2 and GFP in turning associated neurons

    Depositor

    Andrew Leifer

    Article

    Kumar et al PLoS Biol. 2023 Sep 21;21(9):e3002280. d

    Insert

    Guillardia theta anion-conducting channelrhodopsin-2 (GtACR2)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    lim-4

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pT7-EGFP-C1-HsXRN1-del1174-1649_AH

    Plasmid

    #148904

    Purpose

    Mammalian Expression of HsXRN1-del1174-1649

    Depositor

    Elisa Izaurralde

    Article

    Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

    Insert

    HsXRN1-del1174-1649 (XRN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMJB1-RPS22B-Cl

    Plasmid

    #160431

    Purpose

    Expresses "cre-less" yeast RPS22B tagged with mCherry under the GAL1 promoter

    Depositor

    Gary Stormo

    Article

    Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

    Insert

    RPS22B (RPS22B Budding Yeast)
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Removed introns, shuffled codons to change nucleo…

    Promoter

    GAL1 and S. cerevisiae GAL1

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge