We narrowed to 31,051 results for: SON

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pDisplay-NE2m-IRES-mCherry-CAAX

Plasmid

#208690

Purpose

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m and a membrane-localized mcherry in mammalian cells

Depositor

Yulong Li

Article

Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

Insert

GPCR activation based norepinephrine (NE) sensor GRAB_NE2m

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1-msfGFP

Plasmid

#180328

Purpose

mammalian expression of human SEPT9_i1 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pHS0806 His14-bdSUMO-AwaIscB

Plasmid

#176539

Purpose

Bacterial expression of His14-bdSUMO-AwaIscB

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

AwaIscB

Use

CRISPR

Tags

14xHis-bdSUMO tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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PIP2-GFP(ST) sensor

Plasmid

#170356

Purpose

GFP(ST)-PH fusion used to measure realtime changes in PIP2 levels.

Depositor

Kees Jalink

Article

van der Wal et al J Biol Chem. 2001 May 4. 276(18):15337-4

Insert

GFP(ST)-PH (PLCD1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-Hygro-2A-TMRPSS2

Plasmid

#170390

Purpose

Generation of stable lines to enhance SARS-CoV-2 infectivity in ACE2 expressing cells

Depositor

Nir Hacohen

Article

Filbin et al Cell Rep Med. 2021 May 18;2(5):100287. d

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

June 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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P-rTetO-EGFP-T2A-RPL22-3xHA

Plasmid

#170326

Purpose

Tetracycline-inducible expression of HA and EGFP tagged RPL22

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pFl Strep-Sumo-TEV human OAS1

Plasmid

#130895

Purpose

Human oligoadenylate synthase 1 (OAS1) is used to label terminal ADP-riobos

Depositor

Leemor Joshua-Tor

Article

Ando et al Mol Cell. 2019 Feb 21;73(4):845-856.e5.

Insert

oligoadenylate synthase 1 (OAS1 Human)

Tags

Strep_Sumo

Expression

Insect

Promoter

ph

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 AIF-mClover

Plasmid

#129575

Purpose

Expression of mClover fused AIF

Depositor

Stephen Tait

Article

Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

Insert

AIF (AIFM1 Human)

Expression

Mammalian

Mutation

None

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLV302

Plasmid

#119943

Purpose

Expresses N-terminal Npu DnaE Intein-SaKKH-BE3 in mammalian cells

Depositor

Gerald Schwank

Article

Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

Insert

N-terminal SaKKH-BE3(739) - N-Intein (Npu DnaE)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

May 25, 2019

Availability

Academic Institutions and Nonprofits only

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STAgR_Neo

Plasmid

#102992

Purpose

Can be used as backbone for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Type

Empty backbone

Use

CRISPR; Pcr template for stagr vectors

Promoter

U6

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1deltaTMD

Plasmid

#108132

Purpose

Recruitable cytosolic SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-521) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(1-521)

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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RIPK4_pCSdest

Plasmid

#53897

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

RIPK4 (RIPK4 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV sMTase (eGFP)

Plasmid

#89930

Purpose

Expresses sMTase system for targeted DNA methylation in mammalian cells. Contains an eGFP marker expressed off separate promoter.

Depositor

Carl Novina

Article

Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

Insert

dCas9-MC-IRES-MN

Tags

Flag and HA

Expression

Mammalian

Mutation

deactivated Cas9

Promoter

CMV

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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mPA-GFP-Actin-C-18

Plasmid

#57121

Purpose

Localization: Actin, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Insert

Actin (ACTB Human)

Tags

mPA-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAAV-Syn-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

Plasmid

#222742

Purpose

CaST-IRES for neuron expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

Use

AAV

Tags

HA and V5

Expression

Mammalian

Promoter

Synapsin

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB-gDA3m-IRES-mCherry-CAAX

Plasmid

#208692

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m and a membrane-localized mcherry in mammalian cells

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_gDA3m

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pHBS1449 SED1-Lenti

Plasmid

#180824

Purpose

SED1 biosensor for stable integration and expression in mammalian cells

Depositor

Rajat Rohatgi

Article

Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

Insert

SED1 osmosensor

Use

Lentiviral

Promoter

CMV

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pH6HTC_ENO1-Halo

Plasmid

#175330

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

ENO1 (ENO1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgRNA-EF1α-sakkhCas9-HA-NLS-polyA-CBh-EGFP-polyA

Plasmid

#168305

Purpose

mediated knock-in of sgRNA precursor

Depositor

Hui Yang

Article

Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

Insert

SaKKH-EGFP-U6-sgRNA

Use

AAV

Expression

Mammalian

Promoter

EF1alpha, CBh

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-HiBiT-CD63

Plasmid

#162591

Purpose

N-terminally HiBiT-tagged human CD63 under CAG promoter

Depositor

Masaharu Somiya

Article

Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

Insert

human CD63 (CD63 Synthetic, Human, human)

Tags

HiBiT

Expression

Mammalian

Promoter

CAG promoter

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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