We narrowed to 13,804 results for: TIM

1. SPHK2 gRNA (BRDN0001146882)

   Plasmid

   #77096

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SPHK2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SPHK2 (SPHK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. SH3BP4 gRNA (BRDN0001149529)

   Plasmid

   #78087

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SH3BP4

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SH3BP4 (SH3BP4 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. SH3BP4 gRNA (BRDN0001487091)

   Plasmid

   #78089

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SH3BP4

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SH3BP4 (SH3BP4 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSG5-CAGEc-ABL1 (Npu)

   Plasmid

   #241420

   Purpose

   Proximity-triggered Protein Trans-Splicing to generate the splice product BCR-ABL, Figure 3, Extended Figure 6-7 in Nature Chemical Biology 20.10 (2024): 1353-1360.

   Depositor

   Thomas Muir

   Article

   Lee et al bioRxiv [Preprint]. 2023 Jul 10:2023.07.

   Insert

   CAGEc-ABL1 (Npu) (ABL1 Synthetic, Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Sept. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV5-NUP98n-CAGEn-NES (Npu)

   Plasmid

   #241417

   Purpose

   Proximity-triggered Protein Trans-Splicing to generate the splice product MLL-AF9, Extended Figure 4 in Nature Chemical Biology 20.10 (2024): 1353-1360.

   Depositor

   Thomas Muir

   Article

   Lee et al bioRxiv [Preprint]. 2023 Jul 10:2023.07.

   Insert

   NUP98n-CAGEn-NES (Npu) (NUP98 Synthetic, Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV5-BCRn-CAGEn (Npu)

   Plasmid

   #241419

   Purpose

   Proximity-triggered Protein Trans-Splicing to generate the splice product BCR-ABL, Figure 3, Extended Figure 6-7 in Nature Chemical Biology 20.10 (2024): 1353-1360.

   Depositor

   Thomas Muir

   Article

   Lee et al bioRxiv [Preprint]. 2023 Jul 10:2023.07.

   Insert

   BCRn-CAGEn (Npu) (BCR Synthetic, Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCE059-SiT-ddCas12a(E993A)-1xNLS-[Activ]-triplex-DR-M13-DR-pA

   Plasmid

   #202042

   Purpose

   Encodes a DNase-dead AsCpf1 fused to a NLP nuclear localization signal (NLS) and [Activ] transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.

   Depositor

   Darren Cusanovich

   Article

   Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

   Insert

   ddCas12a(E993A)-1xNLS-[Activ]
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCE059-SiT-ddCas12a(E993A)-3xNLS-[Activ]-triplex-DR-M13-DR-pA

   Plasmid

   #202043

   Purpose

   Encodes a DNase-dead AsCpf1 fused to a 3x SV40 nuclear localization signal (NLS) and [Activ] transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.

   Depositor

   Darren Cusanovich

   Article

   Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

   Insert

   ddCas12a(E993A)-3xNLS-[Activ]
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCE059-SiT-ddCas12a(E993A)-3xNLS-VP64-triplex-DR-M13-DR-pA

   Plasmid

   #202044

   Purpose

   Encodes a DNase-dead AsCpf1 fused to a 3x SV40 nuclear localization signal (NLS) and VP64 transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.

   Depositor

   Darren Cusanovich

   Article

   Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

   Insert

   ddCas12a(E993A)-3xNLS-VP64
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTE3: pFuGW-SSX1p-GAL4BD-1X3CLpro consensus CS-VP16AD-SV40NLS

    Plasmid

    #240456

    Purpose

    A synthetic transcription factor (synTF) that contains concensus 3CLpro cleavage site (CS)

    Depositor

    Lior Nissim

    Article

    Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

    Inserts

    SSX1p
    GAL4BD-1X3CLpro consensus CS-VP16AD-SV40NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SSX1 and SSX1p

    Available since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSE163: pFuGW-SSX1p-GAL4BD-1X3CLpro synthetic general CS -VP16AD-SV40NLS

    Plasmid

    #240457

    Purpose

    A synthetic transcription factor (synTF) that contains a synthetic general 3CLpro cleavage site (CS)

    Depositor

    Lior Nissim

    Article

    Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

    Inserts

    SSX1p
    GAL4BD-1X3CLpro synthetic general CS -VP16AD-SV40NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTE5: pFuGW-SSX1p- GAL4BD-4X3CLpro synthetic general CS-VP16AD-SV40NLS

    Plasmid

    #240460

    Purpose

    a synthetic transcription factor (synTF) that contains 4 tandem synthetic general 3CLpro cleavage site (CS).

    Depositor

    Lior Nissim

    Article

    Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

    Inserts

    SSX1p
    GAL4BD-4X3CLpro synthetic general CS-VP16AD-SV40NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV5-NUP98n-CAGEn-NES (Nrdj1)

    Plasmid

    #241418

    Purpose

    Proximity-triggered Protein Trans-Splicing to generate the splice product MLL-AF9, Figure 2 & Extended Figure 4 in Nature Chemical Biology 20.10 (2024): 1353-1360.

    Depositor

    Thomas Muir

    Article

    Lee et al bioRxiv [Preprint]. 2023 Jul 10:2023.07.

    Insert

    NUP98n-CAGEn-NES (Nrdj1) (NUP98 Synthetic, Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-Calmodulin1 (CaM)

    Plasmid

    #240202

    Purpose

    Calmodulin-1 for CaMK2-alpha sensor assays in mammalian cell lines

    Depositor

    Ryohei Yasuda

    Article

    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

    Insert

    calmodulin-1 (Calm1 Mouse)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    July 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-TRE-ETV2

    Plasmid

    #225051

    Purpose

    PiggyBac vector encoding doxycycline-inducible codon-optimized human ETV2

    Depositor

    Sean Palecek, Eric Shusta

    Article

    Ding et al Biotechnol Bioeng. 2025 Jul;122(7):1914-

    Insert

    ETS Variant Transcription Factor 2 (ETV2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon Optimized

    Promoter

    TRE3G

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCas3-CDA1

    Plasmid

    #229534

    Purpose

    pMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    Cas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    

    Use

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Promoter

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. CROP-seq-copGFP-v2-no-scaffold

    Plasmid

    #228520

    Purpose

    CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using copGFP as selection marker

    Depositor

    Jellert Gaublomme

    Article

    Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCA528 IST1 (1-366)

    Plasmid

    #193039

    Purpose

    Bacterial expression plasmid for full-length IST1 (1-366).

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCA528 CAPN7 (1-75)

    Plasmid

    #193031

    Purpose

    Bacterial expression plasmid for CAPN7 (1-75) N-terminal MIT domain

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    Residues 1-75

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCA528 CAPN7 (81-165)

    Plasmid

    #193032

    Purpose

    Bacterial expression plasmid for CAPN7 (81-165) C-terminal MIT domain

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    Residues 81-165

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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