We narrowed to 14,039 results for: TIM

1. ROCK2 gRNA (BRDN0001145177)

   Plasmid

   #76351

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ROCK2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ROCK2 (ROCK2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5 FRT TO myc Separase S1226A/ C2029A

   Plasmid

   #59823

   Purpose

   Allows the integration of myc Separase S1226A/ C2029A in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

   Insert

   Separase (ESPL1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   S1226A/ C2029A

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-K1LM04

   Plasmid

   #103119

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   K1LM04(Cas9 coding gene from Bergeyella zoohelcum ATCC 43767)
   

   Use

   CRISPR; Cloning vector

   Mutation

   human codon-optimized

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. PAK4 gRNA (BRDN0001147629)

   Plasmid

   #77573

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PAK4

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PAK4 (PAK4 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTAMAHISTEV_Ab-PiuA

   Plasmid

   #128957

   Purpose

   expresses Acinetobacter baumannii PiuA in E. coli

   Depositor

   James Naismith

   Article

   Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

   Insert

   TonB-dependent siderophore receptor
   

   Tags

   TEV protease cleavable 7xHis and TamA

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed

   Promoter

   T7

   Available Since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti6.2-coHPDL WT 3xFlag-V5

   Plasmid

   #174129

   Purpose

   Expresses codon-optimized HPDL with a 3xFlag-V5 tag in mammalian cells

   Depositor

   Michael Pacold

   Article

   Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

   Insert

   4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG-V5

   Expression

   Mammalian

   Mutation

   Codon optimized

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. non-targeting control gRNA (BRDN0001147797)

   Plasmid

   #80204

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ZAP70 gRNA (BRDN0001148252)

   Plasmid

   #77955

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ZAP70

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ZAP70 (ZAP70 )
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. non-targeting control gRNA (BRDN0001146436)

   Plasmid

   #80221

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. non-targeting control gRNA (BRDN0001146476)

    Plasmid

    #80194

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAav-MCS-PQS1-3xFLAG

    Plasmid

    #84883

    Purpose

    rAAV-based template for genome engineering of protein C-termini containing PQS1 and 3xFLAG tags and a selection cassette

    Depositor

    Sven Eyckerman

    Article

    Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

    Insert

    Multicloning sites and selection cassette
    

    Use

    AAV

    Promoter

    PGK

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-hNmeCas9-NLS(SV40)-3xFLAG (KAC409)

    Plasmid

    #133790

    Purpose

    CAG promoter expression plasmid for human codon optimized NmeCas9 nuclease with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized NmeCas9
    

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CAG

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. ULK2 gRNA (BRDN0001147288)

    Plasmid

    #77262

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ULK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ULK2 (ULK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT2-7xTcf-NLS-CNL-CP

    Plasmid

    #65715

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL-CP)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, CNL, hCL1-PEST
    

    Use

    Luciferase

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. ROR1 gRNA (BRDN0001162245)

    Plasmid

    #76043

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ROR1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ROR1 (ROR1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MO70-hJunctate - EF-mutant

    Plasmid

    #79597

    Purpose

    Transient and retroviral expression

    Depositor

    Yousang Gwack

    Article

    Srikanth et al Proc Natl Acad Sci U S A. 2012 May 29;10

    Insert

    junctate (ASPH Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    77DAD79 to 77AAA79

    Promoter

    CMV

    Available Since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

17. VRK1 gRNA (BRDN0001147568)

    Plasmid

    #75522

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human VRK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    VRK1 (VRK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. VRK1 gRNA (BRDN0001148483)

    Plasmid

    #75523

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human VRK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    VRK1 (VRK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. INCTbiosyn-pAct-AtINT2

    Plasmid

    #127529

    Purpose

    Plasmid encodes A. thaliana codon optimized Integrase 2.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase 2 coding sequence codon optimized for A. thaliana expression.
    

    Expression

    Plant

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. INCTbiosyn-pAct-AtINT5

    Plasmid

    #127531

    Purpose

    Plasmid encodes A. thaliana codon optimized Integrase 5.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase 5 coding sequence codon optimized for A. thaliana expression.
    

    Expression

    Plant

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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