We narrowed to 10,102 results for: otos

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CMV-mEGFP(A206K)-paCaMKII(T286A)-ShadowG

Plasmid

#167476

Purpose

Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII(T286A)-ShadowG

Tags

ShadowG and mEGFP(A206K)

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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mOrange2-Tubulin-6

Plasmid

#57974

Purpose

Localization: Microtubules, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Tubulin (TUBA1B Human)

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
mPlum-Gamma-Tubulin-17

Plasmid

#55971

Purpose

Localization: Centrosomes, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Gamma-Tubulin (TUBG1 Human)

Tags

mPlum

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
pJH4330

Plasmid

#191700

Purpose

Punc-17::GtACR2::wCherry unc-54 3' UTR C.elegans neuron expression of GtACR2 RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

GtACR2

Tags

wCherry

Expression

Worm

Promoter

Punc-17

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pMGS59(AID-ARF-P2A-Hygromycin)

Plasmid

#138174

Purpose

An AID-ARF-P2A-Hygromycin fusion cassette to tag the protein of interests with auxin-inducible degron and ARF at the c-terminal

Depositor

Michael Guertin

Article

Sathyan et al Curr Protoc Mol Biol. 2020 Sep;132(1):e1

Insert

AID-ARF-P2A-Hygromycin

Tags

AID-ARF clamp degron tag

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJH4329

Plasmid

#191679

Purpose

Prgef-1-GtACR2::wCherry unc-54 3' UTR C.elegans Pan-neural expression of GtACR2 RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

GtACR2

Tags

wCherry

Expression

Worm

Promoter

Pregf-1

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pLexm-gfp-Pept2

Plasmid

#167988

Purpose

expresses rat PepT2 with a c terminal cleavable GFP-His tag.

Depositor

Simon Newstead

Article

Parker et al Sci Adv. 2021 Aug 25;7(35). pii: 7/35/ea

Insert

PepT2 (Slc15a2 Rat)

Tags

cleavable GFP-his8

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-LivePAR(R163A)-Hygro

Plasmid

#187609

Purpose

EGFP fused to the C-terminus of a WWE domain containing the mutation Arg163Ala & a hygromycin resistance cassette.

Depositor

Robert Sobol

Article

Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii

Insert

LivePAR(R163A)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

R163A mutation in the WWE Domain

Promoter

EF1A

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 Orf7b

Plasmid

#158376

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf7b (ORF7b )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pJH4260

Plasmid

#191678

Purpose

Ptwk-40s-GCaMP::wScarlet unc-54 3' UTR C.elegans AVA/other neurons expression GCaMP6s wScarlet

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

GCaMP6s

Tags

wScarlet

Expression

Worm

Promoter

Ptwk-40

Available Since

Nov. 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 NSP15

Plasmid

#158366

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP15 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Nov. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pTS108 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-GFP

Plasmid

#199361

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-SUMOstar-EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTS109 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-GFP

Plasmid

#199362

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TEV-SUMOstar-EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP7

Plasmid

#158358

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP7 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pRSM17

Plasmid

#101641

Purpose

Encodes the fluorescent protein eqFP486 for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

eqFP486

Tags

6XHis

Expression

Bacterial

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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mRFP-dmBACCS2

Plasmid

#67630

Purpose

Drosophila expression of mRFP-dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

mRFP-dmBACCS2

Use

Drosophila expression

Promoter

Drosophila actin gene 5C gene promoter

Available Since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a Gx-NL3

Plasmid

#169108

Purpose

Expresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

Depositor

Maarten Merkx

Article

Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

Insert

protein G - NanoLuc - NanoLuc - NanoLuc

Tags

his-tag and strep-tag

Expression

Bacterial

Mutation

Ala 24 stop

Promoter

T7

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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EYFP-Clathrin-19

Plasmid

#56584

Purpose

Localization: Clathrin Vesicles, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Clathrin (CLTB Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pRSM12

Plasmid

#101643

Purpose

Encodes the fluorescent protein meffCFP for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

meffCFP

Tags

6XHis

Expression

Bacterial

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-P1-N-miRFP670-C

Plasmid

#159438

Purpose

CRISPR knock-in donor construct with fluorescent reporter

Depositor

Jianhua Xing

Article

Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

Insert

miRFP670

Use

CRISPR

Tags

3' linker pair 1, 5' linker pair 1, C t…

Promoter

CMV

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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