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We narrowed to 57,800 results for: plasmid

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Showing: 4561 - 4580 of 57800 results

  2. pLL7 lenti-dcas9-3xflag-blast

    Plasmid
    #112133
    Purpose
    lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)
    Depositor
    Fei-Long Meng
    Publication
    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.
    Insert
    dCas9(D10A, N863A)-T2A-Blast
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A mutants in Cas9
    Promoter
    EF1a
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    112133-plasmid-map-sequence-id-222675

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  3. pX458-ECFP

    Plasmid
    #112220
    Purpose
    Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP
    Depositor
    Yinming Liang
    Publication
    Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ECFP
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    July 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    112220-plasmid-map-sequence-id-215204

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  7. pNO183

    Plasmid
    #101068
    Purpose
    ppsR2-bphP1 bicistronic operon plasmid
    Depositor
    Jeffrey Tabor
    Publication
    Ong et al ACS Synth Biol. 2017 Nov 1. doi: 10.1021
    Inserts
    ppsR2 (ppsR2 Rhodopseudomonas palustris CGA009)
    bphP1
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    insert guanine at position 430 to fix frameshift …
    Available since
    Nov. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    101068-plasmid-map-sequence-id-194033

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  8. pEGFP-Alu-Nicn1

    Plasmid
    #92344
    Purpose
    Plasmid for expression of GFP gene with a Nicn1 gene derived Alu element containing 3'UTR.
    Depositor
    Gordon Carmichael, Ling-Ling Chen
    Publication
    Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nicn1 gene derived Alu element containing 3'UTR (NICN1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    92344-plasmid-map-sequence-id-181521

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  15. pDule-Tet2.0

    Plasmid
    #85496
    Purpose
    Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Publication
    Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044
    Insert
    Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32G L65Q F108S Q109D D158S L162N
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    85496-plasmid-map-sequence-id-169865

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  16. pDule-3-nitroTyrosine (5B)

    Plasmid
    #85498
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Publication
    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32H H70C D158S I159A L162R
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    85498-plasmid-map-sequence-id-169862

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  17. pCDH-EF1-FHC-POLQ-K121M

    Plasmid
    #64877
    Purpose
    lentiviral expression of human POLQ K121M mutant (a mutation in the conserved ATP-binding site of the Walker A motif in the helicase-like domain)
    Depositor
    Richard Wood
    Publication
    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.
    Insert
    POLQ (POLQ Human)
    Use
    Lentiviral
    Tags
    FLAG and HA
    Expression
    Mammalian
    Mutation
    K121M, a mutation in the conserved ATP-binding si…
    Promoter
    EF1alpha
    Available since
    Oct. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    64877-plasmid-map-sequence-id-163756

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  18. pNR220

    Plasmid
    #79541
    Purpose
    Input plasmid: expresses three serine recombinases (TP901, BxbI, and A118) downstream of three inducible promoters.
    Depositor
    Timothy Lu
    Publication
    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.
    Inserts
    TP901
    BxbI
    A118
    Tags
    His tag and LAA degradation tag
    Expression
    Bacterial
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79541-plasmid-map-sequence-id-148753

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  19. pSLC-246

    Plasmid
    #73190
    Purpose
    Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (tse2) under the control of rhamnose induceable promoter (PrhaB) for negative selection.
    Depositor
    Swaine Chen
    Publication
    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8
    Insert
    PrhaB-tse2
    Expression
    Bacterial
    Promoter
    PrhaB
    Available since
    July 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73190-plasmid-map-sequence-id-151367

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  20. pPR219.05

    Plasmid
    #78554
    Purpose
    Plasmid expressing UirR, response regulator and PcsiR1-regulated GFP output
    Depositor
    Jeffrey Tabor
    Publication
    Ramakrishnan et al ACS Synth Biol. 2016 Apr 27.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    uirR
    Use
    Synthetic Biology
    Promoter
    J23115
    Available since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    78554-plasmid-map-sequence-id-408852

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Showing: 4561 - 4580 of 57800 results