1,878 results

1. BC1441-mouse U6-3xsgRNAs (TS5, TS6, TS7)

   Plasmid

   #164040

   Purpose

   Expression of three sgRNAs (sgTS5, sgTS6, sgTS7) for targeting to CRISPR-Tag_V2

   Depositor

   Baohui Chen

   Article

   Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

   Insert

   sgTS5-sgTS6-sgTS7
   

   Use

   CRISPR

   Promoter

   mouse U6

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEY46

   Plasmid

   #191050

   Purpose

   glr-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   glr-1 (glr-1 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEY38

   Plasmid

   #191042

   Purpose

   unc-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   unc-8 (unc-8 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEY13

   Plasmid

   #191019

   Purpose

   Fluorescent reporter driving amphid and phasmid nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   arrd-4 (ZK938.2 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEY20

   Plasmid

   #191026

   Purpose

   gcy-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   gcy-18 (gcy-18 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

   Plasmid

   #108362

   Purpose

   Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans

   Depositor

   Luisa Cochella

   Article

   Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

   Inserts

   Ath-HEN1 (HEN1 Codon optimised for C. elegans, Mustard Weed)
   mCherry
   his-15 (his-15 Nematode)
   unc-54 3' UTR
   

   Tags

   2xFlag, MYC, T2A peptide, and TY1 linker

   Expression

   Worm

   Promoter

   A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…

   Available Since

   May 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-HA-BLMP-1-pcDNA3.1-

   Plasmid

   #52512

   Purpose

   expresses C. elegans BLMP-1 in mammalian cells with FLAG-HA tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   Blmp-1 (blmp-1 Nematode)
   

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   N75S mutation compared GenBank reference sequence…

   Promoter

   CMV

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJR025

   Plasmid

   #198832

   Purpose

   Actin filament and cell cortex localization.

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::act2-venus::let-858 3'UTR
   

   Expression

   Worm

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS1-rab-3P::his-24::CyOFP

   Plasmid

   #124347

   Purpose

   Nuclear Localized Pan-Neuronal CyOFP

   Depositor

   Andrew Leifer

   Insert

   rab-3, CyOFP (rab-3 Nematode)
   

   Expression

   Worm

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. BSP606

    Plasmid

    #122251

    Purpose

    expression vector for mECitrine under EFT3 promoter

    Depositor

    Alexander Mendenhall

    Article

    Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

    Insert

    mECitrine
    

    Expression

    Worm

    Promoter

    EFT3

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRG5271Neo

    Plasmid

    #26387

    Depositor

    Denis Dupuy

    Article

    Giordano-Santini et al Nat Methods. 2010 Sep;7(9):721-3. doi: 1

    Type

    Empty backbone

    Expression

    Bacterial and Worm

    Available Since

    Oct. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSEM229 - [Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR]

    Plasmid

    #159896

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR
    

    Expression

    Worm

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. CH00X

    Plasmid

    #73517

    Purpose

    TagRFP-T_PH pCFJ150 - contains C. elegans codon optimized TagRFP-T

    Depositor

    Bob Goldstein

    Article

    Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

    Insert

    pmex-5::TagRFP-T::PH::tbb-2
    

    Tags

    TagRFP-T

    Promoter

    mes-5

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMLS791

    Plasmid

    #188889

    Purpose

    Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP
    

    Expression

    Worm

    Mutation

    C. elegans codom optimization and intron addition

    Available Since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAP974-1

    Plasmid

    #99497

    Purpose

    TEV::linker::tagRFP::3XFlag

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    TEV::linker::tagRFP::3XFlag
    

    Expression

    Bacterial

    Available Since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSA120

    Plasmid

    #59789

    Purpose

    C. elegans heat-shock driven ZIF-1 and mCherry co-expressed in the same operon

    Depositor

    Jeremy Nance

    Article

    Armenti et al Development. 2014 Dec;141(23):4640-7. do

    Inserts

    hsp-16.41 promoter
    zif-1 coding
    gpd-2/gpd-3 intergenic sequence
    mCherry (worm)
    unc-54 3' UTR
    

    Expression

    Bacterial

    Available Since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJA257

    Plasmid

    #21510

    Depositor

    Julie Ahringer

    Article

    Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

    Insert

    (Gly)5Ala::GFP-F64LS65T::tbb-2 3'UTR
    

    Use

    Multisite gateway

    Available Since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIN4_tsp-6_mScarlet

    Plasmid

    #182935

    Purpose

    Expresses tsp-6::mScarlet under its native promoter (amphid and phasmid ciliated neurons in C. elegans)

    Depositor

    Maureen Barr

    Article

    Nikonorova et al Curr Biol. 2022 May 9;32(9):1924-1936.e6

    Insert

    Ptsp-6-3::tsp-6::flexible linker::mScarlet (C. elegans, genomic)
    

    Tags

    flexible linker (GGGGS)x3 and mScarlet tag

    Expression

    Worm

    Promoter

    tsp-6

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJH2523

    Plasmid

    #191358

    Purpose

    Punc-25-GCaMP3::UrSL2 ::wCherry unc-54 3' UTR C.elegans DD neurou expression of GCaMP3 RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    GCaMP3
    

    Tags

    mCherry

    Expression

    Worm

    Promoter

    Punc-25

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSiM4

    Plasmid

    #199383

    Purpose

    Encodes dual cistronic membrane localized jGCaMP7f and mScarlet-I optimized for C. elegans expression

    Depositor

    Hang Lu

    Insert

    jGCaMP6f::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
    

    Expression

    Bacterial

    Mutation

    no mutations, except added subcellular localizati…

    Available Since

    April 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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