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pSEM246 - MCS mosTI unc-119 target
Plasmid#159821PurposeSingle copy or array insertion by MosTI using split unc-119 selectionDepositorInsertMCS mosTI unc-119 target
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)
Plasmid#159849PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM415 - mosTI unc-119 Pmex-5 - gfp(nls) - tbb-2
Plasmid#182358PurposePositive control for mosTI(unc-119). unc-119 rescue and germline nuclear GFP expression after mosTI insertion.DepositorInsertGFP
UseTagsExpressionWormMutationPromoterPmex-5Available sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2
Plasmid#182351PurposeGermline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]
Plasmid#159798PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMDJ12 - pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]
Plasmid#159799PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM390 - mosTI unc-119 - Peft-3 - NNN - tbb-2
Plasmid#182350PurposeUbiquitous expression cloning vector for mosTI(unc-119). Transgene inserted between Peft-3 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSG020
Plasmid#126503PurposePseudomonas aeruginosa disaggregase ClpG(GI) cloned as ClpG(GI)-6xHisDepositorInsertdisaggregase ClpGgi with 6xHis-tag
UseTagsC-terminal 6xHisExpressionBacterialMutationPromoterPBADAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMDJ21 - pEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]
Plasmid#159810PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM396 - mosTI unc-119 - NNN - operon - gfp - tbb-2
Plasmid#182353PurposeGFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2059 - [1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)
Plasmid#159872PurposeThree-fragment Gateway compatible protein tag for expression in C. elegansDepositorInsert[1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTn7CdrA-gfpC
Plasmid#111616PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.DepositorInsertPcdrA-RBSII-gfp(Mut3)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSAH49 - [PVQp | mScarlet | tbb-2 UTR]
Plasmid#200325PurposemScarlet BioPart for PVQ neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[PVQp | wrmscarlet | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00003755, nlp-17Available sinceJune 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1208
Plasmid#44490DepositorInsertMinimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, unc-119]
UseGateway expression cloneTagsExpressionMutationPromoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJRM3 - [BAGp | mScarlet | tbb-2 UTR]
Plasmid#204616PurposemScarlet BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[BAGp | wrmScarlet | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRM4 - [BAGp | gfp | tbb-2 UTR]
Plasmid#204617Purposegfp BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[BAGp | gfp | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2249 - [1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)
Plasmid#159847PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1985 - [1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)
Plasmid#159846PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2089 - [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)
Plasmid#159874PurposeThree-fragment Gateway compatible protein tag for expression in C. elegansDepositorInsert[1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2211 - [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)
Plasmid#159866PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM398 - mosTI unc-119 - NNN - operon - mScarlet - tbb-2
Plasmid#182355PurposemScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2300 - [1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)
Plasmid#159869PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM387 - mosTI unc-119 - NNN - gfp - nls - tbb-2
Plasmid#182347PurposeGFP transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of gfp(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pISH-Adrb2
Plasmid#105199Purposeto generate a riboprobe for in situ hybridazationDepositorInsertAdrb2 (Adrb2 Mouse)
UseTo ganerate riboprobeTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJL1-EPA-DNNNS-DQNRT
Plasmid#128397PurposeIn vitro expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sitesDepositorInsertExotoxin A
UseTags6xHis tagExpressionBacterialMutation242DNNNS246 and 384DQNRT388 engineered glycosylat…PromoterT7Available sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2240 - [1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop)
Plasmid#159855PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEM386 - mosTI unc-119 - NNN - gfp - tbb-2
Plasmid#182345PurposeGFP fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of gfp and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2060 - [1-2] ENTR - Tag - Snap (intron(1), syntrons(3), no_atg, no_stop)
Plasmid#159873PurposeThree-fragment Gateway compatible protein tag for expression in C. elegansDepositorInsert[1-2] ENTR - Tag - Snap (intron(1), syntrons(3), no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Mac-GFP
Plasmid#58853PurposeAAV-mediated expression of Mac-GFP under the Syn promoterDepositorInsertMac-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterSynAvailable sinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only
