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pTRC-APX(W41F) dimeric

Plasmid

#58499

Purpose

Bacterial expression of APX W41F inducible by IPTG

Depositor

Alice Ting

Article

Dwyer et al Proc Natl Acad Sci U S A. 2014 May 20;11

Insert

pea APX W41F (dimeric)

Tags

His6

Expression

Bacterial

Mutation

W41F

Promoter

trc

Available Since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-Claspin-BPI

Plasmid

#31633

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

Insert

Claspin (CLSPN Human)

Tags

GST

Expression

Bacterial

Mutation

aa 273-492 of claspin

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
PB_rtTA_BsmB-HNRNPU_sgRNA_5

Plasmid

#242900

Purpose

PiggyBac cargo vector with HNRNPU sgRNA 5 for dox-inducible knockdown

Depositor

Mauro Calabrese

Article

Murvin et al bioRxiv 2025.07.16.665154

Insert

HNRNPU (Hnrnpu Mouse)

Use

CRISPR

Promoter

U6

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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PB_rtTA_BsmB-HNRNPU_sgRNA_1

Plasmid

#242901

Purpose

PiggyBac cargo vector with HNRNPU sgRNA 1 for dox-inducible knockdown

Depositor

Mauro Calabrese

Article

Murvin et al bioRxiv 2025.07.16.665154

Insert

HNRNPU (Hnrnpu Mouse)

Use

CRISPR

Promoter

U6

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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PB_rtTA_BsmB-HNRNPU_sgRNA_3

Plasmid

#242902

Purpose

PiggyBac cargo vector with HNRNPU sgRNA 3 for dox-inducible knockdown

Depositor

Mauro Calabrese

Article

Murvin et al bioRxiv 2025.07.16.665154

Insert

HNRNPU (Hnrnpu Mouse)

Use

CRISPR

Promoter

U6

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pET21a Di-iPGM

Plasmid

#180241

Purpose

Bacterial expression plasmid for production of recombinant Dirofilaria immitis iPGM His10

Depositor

James Inglese

Article

Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

Insert

Dirofilaria immitis iPGM-10His

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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sfGFP-SasG(3G)-mCherry

Plasmid

#206945

Purpose

Mammalian expression of heterobifunctional SasG linkers (3G)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (3G)-mCherry

Tags

mCherry and sfGFP

Expression

Mammalian

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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Lnig-INTR-GFP

Plasmid

#208904

Purpose

encodes the inotocin receptor (oxytocin/vasopressin-like) from the ant Lasius niger, tagged with GFP

Depositor

Christian Gruber

Article

Di Giglio et al Sci Rep. 2017 Feb 1;7:41002. doi: 10.103

Insert

INTR

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-hARL1(Q71L)

Plasmid

#206407

Purpose

Mammalian expression of hARL1(Q71L)

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

ARL1 (ARL1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Q71L

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-hARL1(T31N)

Plasmid

#206408

Purpose

Mammalian expression of hARL1(T31N)

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

ARL1 (ARL1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T31N

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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sfGFP-SasG(7G)-mCherry

Plasmid

#206949

Purpose

Mammalian expression of heterobifunctional SasG linkers (7G)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (7G)-mCherry

Tags

mCherry and sfGFP

Expression

Mammalian

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
sfGFP-SasG(6G)-mCherry

Plasmid

#206948

Purpose

Mammalian expression of heterobifunctional SasG linkers (6G)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (6G)-mCherry

Tags

mCherry and sfGFP

Expression

Mammalian

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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sfGFP-SasG(5G)-mCherry

Plasmid

#206947

Purpose

Mammalian expression of heterobifunctional SasG linkers (5G)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (5G)-mCherry

Tags

mCherry and sfGFP

Expression

Mammalian

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
sfGFP-SasG(4G)-mCherry

Plasmid

#206946

Purpose

Mammalian expression of heterobifunctional SasG linkers (4G)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (4G)-mCherry

Tags

mCherry and sfGFP

Expression

Mammalian

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
sfGFP-SasG(2G)-mCherry

Plasmid

#206944

Purpose

Mammalian expression of heterobifunctional SasG linkers (2G)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (2G)-mCherry

Tags

mCherry and sfGFP

Expression

Mammalian

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTV-Flnc-5prime-1000-24MS96T-3F_NeoR

Plasmid

#174881

Purpose

Targeting vector for generating Flnc STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Flnc

Use

Mouse Targeting

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTV-Usp5-5prime-1000-24MS96T-3F_NeoR

Plasmid

#174885

Purpose

Targeting vector for generating Usp5 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Usp5

Use

Mouse Targeting

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTV-Wnk1-5prime-1000-24MS96T-3F_NeoR

Plasmid

#174886

Purpose

Targeting vector for generating Wnk1 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Wnk1

Use

Mouse Targeting

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Full-length P229S talin1

Plasmid

#182993

Purpose

express FL variant P229S talin1 in mammalian cells

Depositor

Vesa Hytönen

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

Talin1 P229S (Tln1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

P229S

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mini-talin P229S (headR13DD P229S)

Plasmid

#182995

Purpose

expression of minitalin P229S in mammalian cells

Depositor

Vesa Hytönen

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

Talin 1 (Tln1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

P229S

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
talin1-head P229S

Plasmid

#182997

Purpose

expression of talin1 head P229S in mammalian cells

Depositor

Vesa Hytönen

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

talin1 head P229S (Tln1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

P229S

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSET-TorA-6xHis-M13-pHCountdown-Calmodulin

Plasmid

#182305

Purpose

E. coli plasmid encoding a fusion between pH-Countdown and the calcium-binding domains of GECO 1.2 with a hexahistidine tag, targeted to the periplasm via the TorA presequence.

Depositor

Andrew York

Article

Seidel et al (2021), doi: 10.5281/zenodo.5810930

Insert

pH-Countdown insertion into GECO 1.2 calcium sensing domains

Tags

A modified TorA periplasmic targeting sequence pl…

Expression

Bacterial

Mutation

Spontaneous mutation of calmodulin's penulti…

Promoter

t7

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCasper-pNos-MCP-mCherry

Plasmid

#180275

Purpose

to epress MCP-mCherry fusion protein in the early fly embryos (driven by maternal nanos driver)

Depositor

Hernan Garcia

Article

Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

Insert

MCP-mCherry

Tags

mCherry

Expression

Insect

Promoter

nanos

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRD393

Plasmid

#168774

Purpose

Expression of mEos3.2_H-NSdbd in Mycobacterium

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

mEos3.2_H-NSdbd

Expression

Bacterial

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a Ec-iPGM-bio

Plasmid

#162575

Purpose

Expresses E. coli iPGM containing a C-terminal biotinylation site and His tag

Depositor

James Inglese

Article

Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

Insert

E. coli iPGM bio-6His

Tags

6His tag, T7 tag, biotinylation sequence, and thr…

Expression

Bacterial

Promoter

T7

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Clim-DC2-H2B2-Venus

Plasmid

#129556

Purpose

pClimDC-H2B:mV nuclear marker

Depositor

Inaki Ruiz-Trillo

Article

Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

Insert

Histone 2B fused to venus

Expression

Bacterial

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Clim-DC2-lifeact-mCherry

Plasmid

#129558

Purpose

pClimDCLifeAct:mCh actin filaments marker

Depositor

Inaki Ruiz-Trillo

Article

Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

Insert

lifeAct fused to mCherry

Expression

Bacterial

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAST-Myc-mSesn2 (1186)

Plasmid

#66920

Purpose

expression of mouse Sesn2 in drosophila

Depositor

Michael Karin, Jun Hee Lee

Article

Lee et al Science. 2010 Mar 5;327(5970):1223-8. do

Insert

Sesn2 (Sesn2 Mouse)

Tags

Myc

Expression

Insect

Promoter

HS promoter

Available Since

Dec. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pET30Xa-SBL1(160E)

Plasmid

#66846

Purpose

Expresses soybean lipoxygenase-1 S160E mutant with N-term Histag

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 (LOC547694, NEWENTRY Glycine max)

Tags

His-tag

Expression

Bacterial

Mutation

S160E

Promoter

T7lac

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET30Xa-SBL1(160S,NoCys)

Plasmid

#66877

Purpose

Expresses a cysteine-free version of WTsoybean lipoxygenase-1 160S (4Cys to Ser) with N-term Histag

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1

Tags

Histag

Expression

Bacterial

Mutation

four (all) cysteines mutated to serine

Promoter

T7lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
tdminiSOG-Tubulin-6

Plasmid

#57808

Purpose

Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Tubulin (TUBA1B Human)

Tags

tdminiSOG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
PA-mCherry-miniSOG-Vinculin-23

Plasmid

#57801

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Vinculin (VCL Human)

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
miniSOG-Vinculin-23

Plasmid

#57780

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Vinculin (VCL Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pTH443-SUP45-G180A

Plasmid

#29386

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

G180A mutant gene including genomic sequence 1432…

Available Since

June 22, 2011

Availability

Academic Institutions and Nonprofits only
pTH398-SUP45-G54D

Plasmid

#29376

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

G54D mutant gene including genomic sequence 1432 …

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
pTH372-SUP45-K60A

Plasmid

#29379

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

K60A mutant gene including genomic sequence 1432 …

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
pTH394-SUP45-L34S

Plasmid

#29373

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

L34S mutant gene (corresponding to the sup45-36ts…

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pTH355-SUP45-T55A

Plasmid

#29377

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

T55A mutant gene including genomic sequence 1432 …

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pTH373-SUP45-S61A

Plasmid

#29380

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

S61A mutant gene including genomic sequence 1432 …

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pTH401-SUP45-R65C

Plasmid

#29381

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

R65C mutant gene (corresponding to the sup45-3 al…

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only

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