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Plasmid#227988PurposeExpresses BioID2-FRB fusion from ADH1 promoter; yeast BioIDDepositorInsertBioID2-FRB
UseTagsHA tagExpressionYeastMutationPromoterADH1Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-BioID2-FKBP-HA3
Plasmid#227990PurposeExpresses BioID2-FKBP fusion from ADH1 promoter; yeast BioIDDepositorInsertBioID2-FKBP
UseTagsHA tagExpressionYeastMutationPromoterADH1Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-BASU-FRB-HA3
Plasmid#227992PurposeExpresses BASU-FRB fusion from ADH1 promoter; yeast BioIDDepositorInsertBASU-FRB
UseTagsHA tagExpressionYeastMutationPromoterADH1Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p- BASU-FKBP-HA3
Plasmid#227994PurposeExpresses BASU-FKBP fusion from ADH1 promoter; yeast BioIDDepositorInsertBASU-FKBP
UseTagsHA tagExpressionYeastMutationPromoterADH1Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-TID-FKBP-HA3
Plasmid#227998PurposeExpresses TurboID-FKBP fusion from ADH1 promoter; yeast BioIDDepositorInsertTurboID-FKBP
UseTagsHA tagExpressionYeastMutationPromoterADH1Available sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-mTID-FRB-HA3
Plasmid#228000PurposeExpresses miniTurboID-FRB fusion from ADH1 promoter; yeast BioIDDepositorInsertminiTurboID-FRB
UseTagsHA tagExpressionYeastMutationPromoterADH1Available sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-mTID-FKBP-HA3
Plasmid#228002PurposeExpresses miniTurboID-FKBP fusion from ADH1 promoter; yeast BioIDDepositorInsertminiTurboID-FKBP
UseTagsHA tagExpressionYeastMutationPromoterADH1Available sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-HIS3p-TID-FRB-HA3
Plasmid#228004PurposeExpresses TurboID-FRB fusion from HIS3 promoter; yeast BioIDDepositorInsertTurboID-FRB
UseTagsHA tagExpressionYeastMutationPromoterHIS3Available sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-HIS3p-TID-FKBP-HA3
Plasmid#228006PurposeExpresses TurboID-FKBP fusion from HIS3 promoter; yeast BioIDDepositorInsertTurboID-FKBP
UseTagsHA tagExpressionYeastMutationPromoterHIS3Available sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-CHA1p-TID-FKBP-HA3
Plasmid#228014PurposeExpresses TurboID-FKBP fusion from CHA1 promoter; yeast BioIDDepositorInsertTurboID-FKBP
UseTagsHA tagExpressionYeastMutationPromoterCHA1Available sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-MET25p-TID-FRB-HA3
Plasmid#228016PurposeExpresses TurboID-FRB fusion from MET25 promoter; yeast BioIDDepositorInsertTurboID-FRB
UseTagsHA tagExpressionYeastMutationPromoterMET25Available sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-MET25p-TID-FKBP-HA3
Plasmid#228018PurposeExpresses TurboID-FKBP fusion from MET25 promoter; yeast BioIDDepositorInsertTurboID-FKBP
UseTagsHA tagExpressionYeastMutationPromoterMET25Available sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP125-134-YFP
Plasmid#224882PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the first 10 amino acids of the proline-rich region (125-134) tagged with YFP using copper.DepositorInsertHtt103QdeltaP125-134 (HTT Human)
UseTagsYFPExpressionYeastMutationdeltaP125-134PromoterCUP1Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP135-170-YFP
Plasmid#224878PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 36 amino acids of the proline-rich region (135-170) tagged with YFP using copper.DepositorInsertHtt103QdeltaP135-170 (HTT Human)
UseTagsYFPExpressionYeastMutationdeltaP135-170PromoterCUP1Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP141-160-YFP
Plasmid#224886PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the middle 20 amino acids of the proline-rich region (141-160) tagged with YFP using copper.DepositorInsertHtt103QdeltaP141-160 (HTT Human)
UseTagsYFPExpressionYeastMutationdeltaP141-160PromoterCUP1Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP125-160-YFP
Plasmid#224883PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the first 36 amino acids of the proline-rich region (125-160) tagged with YFP using copper.DepositorInsertHtt103QdeltaP125-160 (HTT Human)
UseTagsYFPExpressionYeastMutationdeltaP125-160PromoterCUP1Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-M-PRR-Htt97QP4-YFP
Plasmid#224885PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct with the last 46 amino acids of the proline-rich region at the N-terminus, 4 prolines left at the C-terminus using copper.DepositorInsertM-PRR-Htt97QP4 (HTT Human)
UseTagsYFPExpressionYeastMutationN-PRRPromoterCUP1Available sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP141-170-YFP
Plasmid#224880PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 30 amino acids of the proline-rich region (141-170) tagged with YFP using copper.DepositorInsertHtt103QdeltaP141-170 (HTT Human)
UseTagsYFPExpressionYeastMutationdeltaP141-170PromoterCUP1Available sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP161-170-YFP
Plasmid#224876PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 10 amino acids of the proline-rich region (161-170) tagged with YFP using copper.DepositorInsertHTT103QdeltaP161-170 (HTT Human)
UseTagsYFPExpressionYeastMutationdelta161-170PromoterCUP1Available sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF1b-His8-TEV-PI31-1-151
Plasmid#202593PurposeBacterial expression of HsPI31-1-151DepositorInsertPI31 (PSMF1 Human)
UseTags8xHis, TEVExpressionBacterialMutationaa 1-151 onlyPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-DUET-C17orf53_1-241-Strep
Plasmid#211434Purposebacterial expression of the N-terminal amino acids 1-241 of human C17orf53 C-terminally fused to a Strep-TagDepositorInsertC17orf53 (HROB Human)
UseTagsStrep-TagExpressionBacterialMutationDeletion of aa 242-646PromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-DUET1-DHDDS/Sumo-NgBR
Plasmid#203162PurposeExpression of human cis-prenyltransferase complex for purificationDepositorUseTagsHis6-SUMOExpressionBacterialMutationtruncationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsEDC4_977-1401-HsDCP2_395-420_K
Plasmid#146809PurposeBacterial Expression of HsEDC4_977-1401-HsDCP2_385-420DepositorInsertHsEDC4_977-1401-HsDCP2_385-420 (EDC4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsDCP1a_539-582 (MCSII)-mut1_G
Plasmid#146408PurposeBacterial Expression of HsDCP1a_539-582-mut1DepositorInsertHsDCP1a_539-582-mut1 (DCP1A Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsDCP1a_539-582 (MCSII)-mut2_G
Plasmid#146409PurposeBacterial Expression of HsDCP1a_539-582-mut2DepositorInsertHsDCP1a_539-582-mut2 (DCP1A Human)
UseTagsExpressionBacterialMutationone silent mutation T1716G compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsDCP1a_539-582 (MCSII)-mut3_G
Plasmid#146410PurposeBacterial Expression of HsDCP1a_539-582-mut3DepositorInsertHsDCP1a_539-582-mut3 (DCP1A Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsDCP1a_539-582 (MCSII)-WT_G
Plasmid#146411PurposeBacterial Expression of HsDCP1a_539-582DepositorInsertHsDCP1a_539-582 (DCP1A Human)
UseTagsExpressionBacterialMutationone silent mutation T1716G compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsEDC3_192-228-GST-HsRCK_E
Plasmid#146211PurposeBacterial Expression of HsEDC3_192-228-HsRckDepositorInsertHsEDC3_192-228-HsRck (EDC3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsNot10_25-707-HsNot11_260-496_T
Plasmid#147548PurposeBacterial Expression of HsNot10_25-707-HsNot11_260-496DepositorInsertHsNot10_25-707-HsNot11_260-496 (CNOT10 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsNot11_260-496-HsNot10_25-707_T
Plasmid#147549PurposeBacterial Expression of HsNot11_260-496-HsNot10_25-707DepositorInsertHsNot11_260-496-HsNot10_25-707 (CNOT11 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsRCK_296-472-HsEDC3_192-228_E
Plasmid#146180PurposeBacterial Expression of HsRck_296-472-HsEDC3_192-228DepositorInsertHsRck_296-472-HsEDC3_192-228 (DDX6 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsRCK_296-472-HsPatL1_1-59 (MCSII)_D
Plasmid#146104PurposeBacterial Expression of HsRck_296-472-HsPatL1_1-59DepositorInsertHsRck_296-472-HsPatL1_1-59 (DDX6 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsEDC3_192-508del231-263_GST-HsRCK_D
Plasmid#146109PurposeBacterial Expression of HsEDC3_192-508del231-263-HsRckDepositorInsertHsEDC3_192-508del231-263-HsRck (EDC3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmDCP1_1-127-DmXRN1_1312-1383_N
Plasmid#147021PurposeBacterial Expression of DmDCP1_1-127-DmXRN1_1312-1383DepositorInsertDmDCP1_1-127-DmXRN1_1312-1383 (Dcp1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmXRN1_1312-1355-DmDCP1_1-127_N
Plasmid#147054PurposeBacterial Expression of DmXRN1_1312-1355-DmDCP1_1-127DepositorInsertDmXRN1_1312-1355-DmDCP1_1-127 (pcm Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmXRN1_1323-1355-DmDCP1_1-127_N
Plasmid#147055PurposeBacterial Expression of DmXRN1_1323-1355-DmDCP1_1-127DepositorInsertDmXRN1_1323-1355-DmDCP1_1-127 (pcm Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmXRN1_1323-1383-DmDCP1_1-127_N
Plasmid#147056PurposeBacterial Expression of DmXRN1_1323-1383-DmDCP1_1-127DepositorInsertDmXRN1_1323-1383-DmDCP1_1-127 (pcm Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmDCP1_328-366 (MCSII)-mut1_G
Plasmid#146403PurposeBacterial Expression of DmDCP1_328-366-mut1DepositorInsertDmDCP1_328-366-mut1 (Dcp1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmDCP1_328-366 (MCSII)-mut2_G
Plasmid#146404PurposeBacterial Expression of DmDCP1_328-366-mut2DepositorInsertDmDCP1_328-366-mut2 (Dcp1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmDCP1_328-366 (MCSII)-mut3_G
Plasmid#146405PurposeBacterial Expression of DmDCP1_328-366-mut3DepositorInsertDmDCP1_328-366-mut3 (Dcp1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmDCP1_328-366 (MCSII)-WT_G
Plasmid#146406PurposeBacterial Expression of DmDCP1_328-366DepositorInsertDmDCP1_328-366 (Dcp1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsEDC3_192-508-GST-HsRCK_E
Plasmid#146214PurposeBacterial Expression of HsEDC3_192-508-HsRckDepositorInsertHsEDC3_192-508-HsRck (EDC3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS314-U6-G96C/A97C/U100C/U101C
Plasmid#82328PurposeU6 telestem mutant (G96C/A97C/U100C/U101C) for in vivo studiesDepositorInsertU6 RNA with G96C/A97C/U100C/U101C mutation (snR6 Budding Yeast)
UseTagsExpressionYeastMutationU6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U10…PromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS314-U6-A34G/A35G/G96C/A97C
Plasmid#81238PurposeU6 telestem mutant (A34G/A35G/G96C/A97C) for in vivo studiesDepositorInsertU6 RNA with A34G/A35G/G96C/A97C mutation (snR6 Budding Yeast)
UseTagsExpressionYeastMutationU6 telestem mutant (A34G/A35G/G96C/A97C)PromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-Cdc45 (delta 154–164)
Plasmid#126879PurposeExpresses crystallisation construct of human Cdc45 in bacteriaDepositorInsertHuman Cdc45 (CDC45 Human)
UseTagsNo tagExpressionBacterialMutationdeletion amino acid 154-164PromoterT7 promoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS-ATG1-ATG7 3'UTR(406)
Plasmid#122070PurposeExpressing ATG1 with ATG7 3'UTRDepositorInsertserine/threonine protein kinase ATG1 (ATG1 Budding Yeast)
UseTagsExpressionYeastMutationThe native 3'UTR was replaced by ATG7 3'…PromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS-Dhh1-PA(D195A,E196A)(405)
Plasmid#122065PurposeExpressing Dhh1-PADepositorInsertDhh1-PA (DHH1 Budding Yeast)
UseTagsPAExpressionYeastMutationAspartic acid 195 to alanine; Glutamic acid 196 t…PromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS-Dhh1-PA(S226A,T228A)(405)
Plasmid#122066PurposeExpressing Dhh1-PADepositorInsertDhh1-PA (DHH1 Budding Yeast)
UseTagsPAExpressionYeastMutationSerine 226 to alanine; Threonine 228 to alaninePromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKP105 (pRS416-YBR139Wp-YBR139W-PA-ADH1t)
Plasmid#106461PurposeExpresses Atg42/Ybr139w with a C-terminal PA tag in yeast cellsDepositorInsertYBR139W
UseTagsPAExpressionBacterial and YeastMutationPromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP135 (pRS406-YBR139Wp-YBR139W-PA-ADH1t)
Plasmid#106470PurposeExpresses Atg42/Ybr139w with a C-terminal PA tag in yeast cellsDepositorInsertYBR139W
UseTagsPAExpressionBacterial and YeastMutationPromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only