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pET-FLAG-eSpCas9

Plasmid

#126769

Purpose

Expression of increased fidelity eSpCas9 in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

eSpCas9

Use

CRISPR

Tags

3xFLAG, 6xHis, MBP, and NLS

Expression

Bacterial

Mutation

K848A, K1003A, R1060A

Promoter

T7

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TAL2020

Plasmid

#39378

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-11-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
TAL2021

Plasmid

#39379

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-11-Right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer

Plasmid

#172501

Purpose

Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

DisCas7-11 full locus with Golden gate acceptor site for spacer

Expression

Bacterial

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.RMacL3-11.EGFP.BCB12.WPRE

Plasmid

#229626

Purpose

L3 pyramidal neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Publication

He et al Neuron. 2025 May 21;113(10):1548-1561.e8

Insert

RMacL3-11.EGFP.BCB12

Expression

Mammalian

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.RMacL5ET-11.EGFP.BCC4.WPRE

Plasmid

#229637

Purpose

L5 extratelencephalic projection neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Publication

He et al Neuron. 2025 May 21;113(10):1548-1561.e8

Insert

RMacL5ET-11.EGFP.BCC4

Expression

Mammalian

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Tc1-11_Xt

Plasmid

#217150

Purpose

Mammalian expression vector for expression of Tc1-11_Xt transposase (CMV promoter)

Depositor

Haoyi Wang

Publication

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

Tc1-11_Xt transposase

Expression

Mammalian

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-hAT-11_AMi

Plasmid

#217199

Purpose

Mammalian expression vector for expression of hAT-11_AMi transposase (CMV promoter)

Depositor

Yong E Zhang

Publication

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

hAT-11_AMi transposase

Expression

Mammalian

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pST_PvLEA4_repeats_k3_11 (pBS0761)

Plasmid

#185243

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_11. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k3_11

Expression

Mammalian

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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AA226

Plasmid

#216027

Purpose

Fragmid fragment: (Cas protein) Cas7-11

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas7-11_v1.1 [Di]

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCSFLPe

Plasmid

#31130

Depositor

Gerhart Ryffel

Publication

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

FLP recombinase

Expression

Mammalian

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Lact-C2-GFP-p416

Plasmid

#22853

Depositor

Sergio Grinstein

Publication

Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

Insert

Lactadherin-C2

Tags

GFP

Expression

Yeast

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pSR59.4

Plasmid

#63175

Purpose

Expresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoter

Depositor

Jeffrey Tabor

Publication

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

OmpR (ompR )
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pENTREGFP2

Plasmid

#22450

Depositor

Nathan Lawson

Publication

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

EGFP (eGFP )

Available since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pDF0237 pCMV - dead huDisCas7-11 - NES - huADAR2

Plasmid

#172510

Purpose

Encodes human codon optimized catalytically dead DisCas7-11-NES-huADAR2 fusion protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pCMV - dead huDisCas7-11 - NES - huADAR2

Expression

Mammalian

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-HA-hTAF1

Plasmid

#17997

Depositor

Robert Tjian

Publication

Ruppert et al Nature. 1993 Mar 11. 362(6416):175-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hTAF1 (TAF1 Human)

Tags

HA

Expression

Mammalian

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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p3259 pCMV hUBC9 mt HA

Plasmid

#14439

Depositor

Peter Howley

Publication

Yasugi et al Nucleic Acids Res. 1996 Jun 1. 24(11):20

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

UBC9 C93S (UBE2I Human)

Tags

HA

Expression

Mammalian

Mutation

Cys93Ser mutation (TGC changed to AGC)

Promoter

CMV

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCM5.57

Plasmid

#19378

Depositor

Geraldine Seydoux

Publication

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

spe-11 3'utr in pDONRP2RP3

Expression

Worm

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Plk4(Sak) wt (Nigg HR9)

Plasmid

#41165

Depositor

Erich Nigg

Publication

Habedanck et al Nat Cell Biol. 2005 Nov;7(11):1140-6.

Insert

Plk4 (PLK4 Human)

Tags

3xMyc and PreScission Site

Expression

Mammalian

Promoter

CMV

Available since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCMV5B-FLAG-SARA

Plasmid

#11738

Depositor

Jeff Wrana

Publication

Tsukazaki et al Cell. 1998 Dec 11. 95(6):779-91.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARA (ZFYVE9 Human)

Tags

Flag

Expression

Mammalian

Available since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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